Effects of hyperosmotic stress on cultured airway epithelial cells

Nilsson, Hans C.; Dragomir, Anca; Ahlander, Anders; Johannesson, Marie; Roomans, Godfried M.

doi:10.1007/s00441-007-0482-7

Cited by 31 publications

(23 citation statements)

References 35 publications

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“…Electrophysiology experiments cannot be done on the coral oral tissue owing to technical limitations, but structural similarities between coral and anemone oral tissues [42] suggest that results should be comparable, and if this was the case the higher resistance of the tissues of S. pistillata would be due to the paracellular junctions of the aboral tissue. In epithelia, permeability due to the paracellular pathway is known to increase under hyperosmotic conditions with non-electrolyte solutions by opening junctions [32,43]. In this study, we have observed that a hyperosmotic shock leads to a decrease in microcolony resistance, and thus an increase in tissue permeability.…”

mentioning

confidence: 56%

“…Since it was impossible to separate the oral tissue from the aboral tissue in S. pistillata, we measured the resistance of the oral tissue of the sea anemone A. viridis and obtained a value of 15 + 3 Ohm cm 2 . We then performed a hyperosmotic shock, which is usually used to determine if junctions are involved in the paracellular resistance of tissues [32]. When the hyperosmotic shock was performed in the hemi-chamber facing the tissues (apex) of a microcolony, the resistance decreased as a function of time (electronic supplementary material, figure S3).…”

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confidence: 99%

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Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification

et al. 2011

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The mechanisms behind the transfer of molecules from the surrounding sea water to the site of coral calcification are not well understood, but are critical for understanding how coral reefs are formed. We conducted experiments with the fluorescent dye calcein, which binds to calcium and is incorporated into growing calcium carbonate crystals, to determine the permeability properties of coral cells and tissues to this molecule, and to determine how it is incorporated into the coral skeleton. We also compared rates of calcein incorporation with rates of calcification measured by the alkalinity anomaly technique. Finally, by an electrophysiological approach, we investigated the electrical resistance of coral tissues in order to better understand the role of tissues in ionic permeability. Our results show that (i) calcein passes through coral tissues by a paracellular pathway, (ii) intercellular junctions control and restrict the diffusion of molecules, (iii) intercellular junctions should have pores of a size higher than 13 Å and lower than 20 nm, and (iv) the resistance of the tissues owing to paracellular junctions has a value of 477 + 21 Ohm cm 2 . We discuss the implication of our results for the transport of calcium involved in the calcification process.

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confidence: 56%

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confidence: 99%

Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification

et al. 2011

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“…The presence of mannitol, which was the sole extra excipient in the powder formulations, could partly explain the increase in transfection efficiency. Hyperosmotic mannitol solution is known to be able to open up tight junctions between adjacent cells, therefore increasing the accessibility of DNA complexes to the cell surface [47,48] and the increase in transfection appeared in a mannitol dose dependent manner. However the mannitol in our powder formulations would have been present at a final concentration of 0.25% (w/v) and this may be too low to produce any prominent osmotic effect to the cells.…”

Section: Discussionmentioning

confidence: 99%

Formulation of pH responsive peptides as inhalable dry powders for pulmonary delivery of nucleic acids

Liang

Kwok

Chow

et al. 2014

European Journal of Pharmaceutics and Biopharmaceutics

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Nucleic acids have the potential to be used as therapies or vaccines for many different types of disease but delivery remains the most significant challenge to their clinical adoption. pH responsive peptides containing either histidine or derivatives of 2,3-diaminopropionic acid (Dap) can mediate effective DNA transfection in lung epithelial cells with the latter remaining effective even in the presence of lung surfactant containing bronchoalveolar fluid (BALF), making this class of peptides attractive candidates for delivering nucleic acids to lung tissues. To further assess the suitability of pH responsive peptides for pulmonary delivery by inhalation, dry powder formulations of pH responsive peptides and plasmid DNA, with mannitol as carrier, were produced by either spray drying (SD) or spray freeze drying (SFD). The properties of the two types of powders were characterised and compared using scanning electron microscopy (SEM), next generation impaction (NGI), gel retardation and in vitro transfection via a twin-stage impinger (TSI) following aerosolisation by a dry powder inhaler (Osmohaler™). Although the aerodynamic performance and transfection efficacy of both powders were good, the overall performance revealed SD powders to have a number of advantages over SFD powders and are the more effective formulation with potential for efficient nucleic acid delivery through inhalation.

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“…Similarly, increasing the osmolarity from FW to 305mOsm L15 (48h recovery from 3mOsm) decreased claudin 28b fluorescence in control cultures significantly, which was in line with reduced TER values. In airway epithelial cells hyperosmotic exposure decreased claudin-4 in the tight junctions as well (Nilsson et al, 2007).…”

Section: Claudin 28b Tight Junction Fluorescence Correlates With Epitmentioning

confidence: 94%

Claudin 28b and F-actin are involved in rainbow trout gill pavement cell tight junction remodeling under osmotic stress

Sandbichler

Egg

Schwerte

et al. 2011

Journal of Experimental Biology

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SUMMARYPermeability of rainbow trout gill pavement cells cultured on permeable supports (single seeded inserts) changes upon exposure to freshwater or treatment with cortisol. The molecular components of this change are largely unknown, but tight junctions that regulate the paracellular pathway are prime candidates in this adaptational process. Using differential display polymerase chain reaction we found a set of 17 differentially regulated genes in trout pavement cells that had been exposed to freshwater apically for 24h. Five genes were related to the cell-cell contact. One of these genes was isolated and identified as encoding claudin 28b, an integral component of the tight junction. Immunohistochemical reactivity to claudin 28b protein was concentrated in a circumferential ring colocalized to the cortical F-actin ring. To study the contribution of this isoform to changes in transepithelial resistance and Phenol Red diffusion under apical hypo-or hyperosmotic exposure we quantified the fluorescence signal of this claudin isoform in immunohistochemical stainings together with the fluorescence of phalloidin-probed F-actin. Upon hypoosmotic stress claudin 28b fluorescence and epithelial tightness remained stable. Under hyperosmotic stress, the presence of claudin 28b at the junction significantly decreased, and epithelial tightness was severely reduced. Cortical F-actin fluorescence increased upon hypo-osmotic stress, whereas hyperosmotic stress led to a separation of cortical F-actin rings and the number of apical crypt-like pores increased. Addition of cortisol to the basolateral medium attenuated cortical F-actin separation and pore formation during hyperosmotic stress and reduced claudin 28b in junctions except after recovery of cells from exposure to freshwater. Our results showed that short-term salinity stress response in cultured trout gill cells was dependent on a dynamic remodeling of tight junctions, which involves claudin 28b and the supporting F-actin ring.

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Effects of hyperosmotic stress on cultured airway epithelial cells

Cited by 31 publications

References 35 publications

Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification

Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification

Formulation of pH responsive peptides as inhalable dry powders for pulmonary delivery of nucleic acids

Claudin 28b and F-actin are involved in rainbow trout gill pavement cell tight junction remodeling under osmotic stress

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