Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium

Tiboldi, Akos; Hunyadi‐Gulyás, Éva; Wohlrab, Peter; Schmid, Johannes A.; Markstaller, Klaus; Klein, Klaus Ulrich; Tretter, Verena

doi:10.3390/antiox11122349

Cited by 3 publications

(1 citation statement)

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“…In contrast with well characterized neuro- and physiological outcomes of exposure to steady-state F i O 2 , fewer studies have focused upon the outcomes of exposure to non-steady-state F i O 2 . Those findings, often derived from cell [ 16 ], rodent, or swine models [ 17 ] reveal inflammation of the pulmonary epithelium. Those observations concur with studies by Formenti et al [ 18 , 19 ] revealing that non-steady-state F i O 2 levels, induced by altering the ventilatory inspiratory and expiratory ratios, lead to non-steady-state arterial blood oxygen (P a O 2 ) levels [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Exposure to Non-Steady-State Oxygen Is Reflected in Changes to Arterial Blood Gas Values, Prefrontal Cortical Activity, and Systemic Cytokine Levels

Damato,

Piktel,

Margevicius

et al. 2024

IJMS

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Onboard oxygen-generating systems (OBOGSs) provide increased inspired oxygen (FiO2) to mitigate the risk of neurologic injury in high altitude aviators. OBOGSs can deliver highly variable oxygen concentrations oscillating around a predetermined FiO2 set point, even when the aircraft cabin altitude is relatively stable. Steady-state exposure to 100% FiO2 evokes neurovascular vasoconstriction, diminished cerebral perfusion, and altered electroencephalographic activity. Whether non-steady-state FiO2 exposure leads to similar outcomes is unknown. This study characterized the physiologic responses to steady-state and non-steady-state FiO2 during normobaric and hypobaric environmental pressures emulating cockpit pressures within tactical aircraft. The participants received an indwelling radial arterial catheter while exposed to steady-state or non-steady-state FiO2 levels oscillating ± 15% of prescribed set points in a hypobaric chamber. Steady-state exposure to 21% FiO2 during normobaria produced arterial blood gas values within the anticipated ranges. Exposure to non-steady-state FiO2 led to PaO2 levels higher upon cessation of non-steady-state FiO2 than when measured during steady-state exposure. This pattern was consistent across all FiO2 ranges, at each barometric condition. Prefrontal cortical activation during cognitive testing was lower following exposure to non-steady-state FiO2 >50% and <100% during both normobaria and hypobaria of 494 mmHg. The serum analyte levels (IL-6, IP-10, MCP-1, MDC, IL-15, and VEGF-D) increased 48 h following the exposures. We found non-steady-state FiO2 levels >50% reduced prefrontal cortical brain activation during the cognitive challenge, consistent with an evoked pattern of neurovascular constriction and dilation.

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Section: Introductionmentioning

confidence: 99%

Exposure to Non-Steady-State Oxygen Is Reflected in Changes to Arterial Blood Gas Values, Prefrontal Cortical Activity, and Systemic Cytokine Levels

Damato,

Piktel,

Margevicius

et al. 2024

IJMS

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Perioperative oxygen concentration and postoperative organ damage in pediatric neurosurgery: a retrospective cohort study

Hu,

Wang,

Wang

et al. 2024

Preprint

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Purpose To investigate the correlation between perioperative oxygen concentration and postoperative organ damage and other adverse reactions in children undergoing neurosurgery. Materials and methods This study was a single-center retrospective cohort study that included 512 children undergoing neurosurgery, aged ≤14 years old, and with an operative duration ≥2 hours who were transferred to the Postanesthesia Care Unit (PACU) of the Department of Anesthesiology, Beijing Tiantan Hospital, postoperatively during the period of October 2018 to September 2023. The medical records of the included children during hospitalization were meticulously reviewed and systematically collected through the Hospital Information System (His).The area under oxygen concentration-time curve AUCFIO2= the product of intraoperative oxygen concentration (percentage) and duration of anesthesia (minutes) was calculated, and then 512 children were divided into 3 groups according to the trichotomies of the AUCFIO2 (Group1:AUCFIO2<8720%min, Group2: 8720% min≤AUCFIO2≤13800% min, Group 3: AUCFIO2 > 13800% min) for inclusion in the data analysis. Results The incidence of Emergency delirium was 24%, 26.9% and 19.4%, and the incidence of acute kidney injury was 0.6%, 2.3% and 0.6% in the three groups, respectively. There was no significant difference between the three groups (P> 0.05). The incidence of postoperative pulmonary complications in the three groups was 20.6%, 20.8% and 31.5%, respectively, and there was a statistically significant difference between the three comparisons (P<0.05). There were no significant differences in nausea, vomiting, hypoxemia, VAS score and PACU residence time among the three groups during PACU (P>0.05), and there was a statistically significant differences between infections and hospital stay during hospitalization (P<0.05). Conclusions Although the oxygen concentration AUCFIO2 was not correlated with the occurrence of both postoperative AKI and ED, the longer the exposure time of conventional oxygen administration strategy, the higher the incidence of postoperative pulmonary complications and postoperative infections, and the longer the hospitalization time of children.

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Deciphering regulatory patterns in a mouse model of hyperoxia-induced acute lung injury

Chen,

Liu,

Qin

et al. 2024

PeerJ

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Background Oxygen therapy plays a pivotal role in treating critically ill patients in the intensive care unit (ICU). However, excessive oxygen concentrations can precipitate hyperoxia, leading to damage in multiple organs, with a notable effect on the lungs. Hyperoxia condition may lead to hyperoxia-induced acute lung injury (HALI), deemed as a milder form of acute respiratory distress syndrome (ARDS). Given its clinical importance and practical implications, there is a compelling need to investigate the underlying pathogenesis and comprehensively understand the regulatory mechanisms implicated in the development of HALI Results In this study, we conducted a mouse model with HALI and performed regulatory mechanism analysis using RNA-seq on both HALI and control group. Comprehensive analysis revealed 727 genes of significant differential expression, including 248 long non-coding RNAs (lncRNAs). Also, alternative splicing events were identified from sequencing results. Notably, we observed up-regulation or abnormal alternative splicing of genes associated with immune response and ferroptosis under hyperoxia conditions. Utilizing weighted gene co-expression network analysis (WGCNA), we ascertained that genes involved in immune response formed a distinct cluster, showcasing an up-regulated pattern in hyperoxia, consistent with previous studies. Furthermore, a competing endogenous RNA (ceRNA) network was constructed, including 78 differentially expressed mRNAs and six differentially expressed lncRNAs, including H19. These findings uncover the intricate interplay of multiple transcriptional regulatory mechanisms specifically tailored to the pulmonary defense against HALI, substantiating the importance of these non-coding RNAs in this disease context. Conclusions Our results provide new insights into the potential mechanisms and underlying pathogenesis in the development of HALI at the post-transcriptional level. The findings of this study reveal potential regulatory interactions and biological roles of specific lncRNAs and genes, such as H19 and Sox9, encompassing driven gene expression patterns, alternative splicing events, and lncRNA-miRNA-mRNA ceRNA networks. These findings may pave the way for advancing therapeutic strategies and reducing the risk associated with oxygen treatment for patients.

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Effects of Hyperoxia and Hyperoxic Oscillations on the Proteome of Murine Lung Microvascular Endothelium

Cited by 3 publications

References 50 publications

Exposure to Non-Steady-State Oxygen Is Reflected in Changes to Arterial Blood Gas Values, Prefrontal Cortical Activity, and Systemic Cytokine Levels

Exposure to Non-Steady-State Oxygen Is Reflected in Changes to Arterial Blood Gas Values, Prefrontal Cortical Activity, and Systemic Cytokine Levels

Perioperative oxygen concentration and postoperative organ damage in pediatric neurosurgery: a retrospective cohort study

Deciphering regulatory patterns in a mouse model of hyperoxia-induced acute lung injury

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