Effects of hypertonic dextrose injections in the rabbit carpal tunnel

Yoshii, Yukie; Zhao, Chunfeng; Schmelzer, James D.; Low, Phillip A.; An, Kai Nan; Amadio, Peter C.

doi:10.1002/jor.21297

Cited by 36 publications

(47 citation statements)

References 24 publications

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“…Based on previous studies [10,18], a sample size of 12 was needed in the mechanical testing group, and 25 were needed for EP testing. We estimated that eight specimens would be needed for histology and noted that the preparations for routine staining, nerve histology, and EM all required different fixation and so could not all be done on the same specimens.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Time after Shear Injury on the Subsynovial Connective Tissue and Median Nerve within the Rabbit Carpal Tunnel

Vanhees

Chikenji

Thoreson

et al. 2012

Hand (New York, N,Y.)

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Background The most prominent nonneurological finding in the common compression neuropathy carpal tunnel syndrome (CTS) is fibrosis of the subsynovial connective tissue (SSCT). Recently, a rabbit model of CTS has been developed, based on the hypothesis that SSCT injury and subsequent fibrosis cause nerve compression. The purpose of this study was to evaluate the effects in this model at earlier and later time points than have heretofore been reported. Methods Sixty rabbits were operated on and observed at two different time periods: 6 and 24 weeks. Nerve electrophysiology (EP), SSCT histology, and SSCT mechanical properties were assessed. Results There was no significant difference in median motor nerve amplitude or latency at either time point. The total cell density in the SSCT was significantly higher at 6 and 24 weeks compared to controls. The mean size of the collagen fibrils in the SSCT was higher 6 and 24 weeks after surgery compared to controls. Both the ultimate load and the total energy absorption of the SSCT were significantly higher at 6 and 24 weeks compared to controls. Conclusions In this model, there were signs of SSCT fibrosis and histology changes at 6 weeks, which persist after 24 weeks. Thus, this model leads to sustained SSCT fibrosis, which is one characteristic of human CTS. However, no significant EP changes were found at these two time points, which is in contrast to the findings reported previously for this model at 12 weeks. The significance of the differences in EP findings will be the subject of future studies.

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Section: Discussionmentioning

confidence: 99%

The Effect of Time after Shear Injury on the Subsynovial Connective Tissue and Median Nerve within the Rabbit Carpal Tunnel

Vanhees

Chikenji

Thoreson

et al. 2012

Hand (New York, N,Y.)

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“…6,11 A rabbit CTS model has been developed in which SSCT fibrosis is induced by injection of hypertonic dextrose. 12-14 …”

Section: Introductionmentioning

confidence: 99%

Collagen gel contraction as a measure of fibroblast function in an animal model of subsynovial connective tissue fibrosis

Yang

Thoreson

Gingery

et al. 2015

Journal Orthopaedic Research

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Carpal tunnel syndrome (CTS) is a peripheral neuropathy characterized by non-inflammatory fibrosis of the subsynovial connective tissues (SSCT). A rabbit model of CTS was developed to test the hypothesis that SSCT fibrosis causes the neuropathy. We used a cell-seeded collagen-gel contraction model to characterize the fibrosis in this model in terms of cellular mechanics, specifically to compare the ability of SSCT cells from the rabbit model and normal rabbits to contract the gel, and to assess the effect of transforming growth factor-β1,which is upregulated in CTS, on these cells. SSCT fibrosis was induced in six retired breeder female rabbits which were sacrificed at 6 weeks (N=3) and 12 weeks (n=3). An additional two rabbits served as controls. SSCT was harvested according to a standard protocol. Gels seeded with SSCT cells from rabbits sacrificed at 6 weeks had significantly higher tensile strength (p<0.001) and Young’s modulus (p<0.001) than gels seeded with cells from rabbits sacrificed at 12 weeks or control animals. TGF-β1 significantly increased the decay time constant (p<0.001), tensile strength (p<0.001) and Young’s modulus (p<0.001) regardless of the cell source. This model may be useful in screening therapeutic agents that may block SSCT fibrosis, identifying possible candidates for CTS treatment.

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“…It will eventually thicken and strengthen injured tendons and ligaments. The recent animal study confirmed the presence of fibrous tissue in the carpal tunnel after injection of hyperosmolar dextrose (21). This is a retrospective pilot study conducted on a small sample of participants.…”

Section: Discussionmentioning

confidence: 56%