Effects of hypo- or hyperosmotic stress on lipid synthesis and gluconeogenic activity in tissues of the crab Neohelice granulata

Martins, Tiago Leal; Chittó, Ana Lúcia F.; Rossetti, Camila Lüdke; Brondani, Camila K.; Kucharski, Luiz Carlos; Silva, Roselis S.M. da

doi:10.1016/j.cbpa.2010.11.023

Cited by 28 publications

(25 citation statements)

References 31 publications

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“…Estuarial crabs Neohelice granulata (Dana, 1851) used lipids during ionic hyporegulation, but during hyperegulation lipid reserves remained unaltered (Luvizotto Santos et al, 2003). In the same species exposed to high salinities, higher lipid storage from free amino acids was seen both in hepatopancreas and muscle, in relation to the needs for osmotic regulation (Martins et al, 2011).…”

Section: Discussionmentioning

confidence: 91%

“…Glycogen can be directly used to augment the glucose in hemolymph, while proteins can be used for the same purpose after gluconeogenesis. In fact, the crustacean hepatopancreas is able to carry out gluconeogenesis from muscle protein as substrate, to elevate glycemia to support a higher metabolic demand (Martins et al, 2011). An early utilization of glycogen has been reported in the white shrimp, Litopenaeus vannamei, acclimated from 28 to 13°C; additionally, a further utilization of proteins take place in the same species at longer acclimation time (Zhou et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Effects of both ecdysone and the acclimation to low temperature, on growth and metabolic rate of juvenile freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae)

Chaulet

Vatnick

Rodríguez

2013

Iheringia, Sér. Zool.

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ABSTRACT. Growth, metabolic rate, and energy reserves of Cherax quadricarinatus (von Martens, 1868) juveniles were evaluated in crayfish acclimated for 16 weeks to either 25°C (temperature near optimum) or 20°C (marginal for the species). Additionally, the modulating effect of ecdsyone on acclimation was studied. After 12 weeks of exposure, weight gain of both experimental groups acclimated to 25°C (control: C25, and ecdysone treated: E25) was significantly higher than that of those groups acclimated to 20°C (C20 and E20). A total compensation in metabolic rate was seen after acclimation from 25°C to 20°C; for both the control group and the group treated with ecdysone. A Q 10 value significantly higher was only observed in the group acclimated to 20°C and treated with ecdysone. A reduction of glycogen reserves in both hepatopancreas and muscle, as well as a lower protein content in muscle, was seen in both groups acclimated to 20°C. Correspondingly, glycemia was always higher in these groups. Increased lipid levels were seen in the hepatopancreas of animals acclimated to 20°C, while a higher lipid level was also observed in muscle at 20°C, but only in ecdysone-treated crayfish. KEYWORDS.Energy reserves, growth, metabolic rate, Q 10 .RESUMEN. Efectos de ecdisona y de la aclimatación a baja temperatura, sobre el crecimiento y la tasa metabólica de juveniles de la langosta de agua dulce Cherax quadricarinatus (Decapoda, Parastacidae). Se evaluaron las reservas energéticas, el crecimiento y la tasa metabólica de langostas juveniles de agua dulce Cherax quadricarinatus (von Martens, 1868), aclimatadas durante 16 semanas tanto a 25°C (temperatura cercana al óptimo) como a 20°C (temperatura marginal para la especie). Adicionalmente, se evaluó el efecto modulador de la ecdisona sobre la aclimatación. Luego de 12 semanas de exposición, la ganancia en peso de ambos grupos experimentales aclimatados a 25°C (control: C25, y tratados con ecdisona: E25) fue significativamente mayor que la de los grupos aclimatados a 20°C (C20 and E20). Se verificó una compensación total en la tasa metabólica, luego de la aclimatación desde 25 a 20°C, tanto para el grupo control como para el tratado con ecdisona. Solamente se observó un valor de Q 10 significativamente mayor en el grupo aclimatado a 20°C y tratado con ecdisona. En ambos grupos aclimatados a 20°C, se determinó un reducción en la reservas de glucógeno, tanto en hepatopancreas como en músculo, así como un menor contenido de proteína en músculo. Correspondiente, la glucemia resultó siempre más elevada en esos grupos, que también mostraron un mayor nivel de lípidos en el hepatopancreas, siendo mayor el nivel de lípidos en músculo sólo para los juveniles aclimatados a 20°C y tratados con ecdisona. PALABRAS-CLAVE.Reservas energéticas, crecimiento, tasa metabólica, Q 10 .Metabolic rate of invertebrates depends on several intrinsic and extrinsic factors. Intrinsic factors include: age, gender, weight, degree of locomotor activity and internal work. Extrinsic factors include: ambient te...

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Effects of both ecdysone and the acclimation to low temperature, on growth and metabolic rate of juvenile freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae)

Chaulet

Vatnick

Rodríguez

2013

Iheringia, Sér. Zool.

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“…Fish or shrimp gills are influenced more by osmotic changes than by dietary conditions alone. Change in gills FBPase activity in response to osmotic changes was shown in Neohelice granulata (Kumar et al, ; Martins et al, ). Therefore, gills demonstrate consistent FBPase activity across all experimental groups in this study suggesting that more conducive water conditions of M. rosenbergii farm were encountered for this experiment than the influence on feed or stock mediated variations .…”

Section: Discussionmentioning

confidence: 96%

Effect of different stock types and dietary protein levels on key enzyme activities of glycolysis‐gluconeogenesis, the pentose phosphate pathway and amino acid metabolism inMacrobrachiumrosenbergii

et al. 2019

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A feeding trial was conducted to study the effect of dietary protein (DP) levels on the enzyme activities of different stocks of Macrobrachium rosenbergii juveniles, fed two diets prawn type diets either at optimum (ODP, 32%) or sub‐optimum (SDP, 27%) dietary protein. The activity of key enzymes: glycolysis, gluconeogenesis, amino acid catabolism, pentose phosphate pathway, and energy metabolism was examined on different stocks of M. rosenbergii juveniles in various tissues (muscle, hepatopancreas, and gill). Wild juveniles of M. rosenbergii were collected from the west coast of India, Gujarat (G), Maharashtra (M) and from the east coast of India, Andhra Pradesh (A), and raised in culture ponds of 200 m2 at 1 juvenile m‐2. The experiment was conducted for a period of 60 days. A Visible Implant Elastomer (VIE) Tag was injected into different prawn juveniles’ stocks A, (3.06–3.10 g), M, (0.80–1.01 g) and G (0.90–1.06 g) for the individual identification of each class. All the animals were acclimatized for 7 days before being released into the pond. Each of the two diets, the first with 27% SDP, termed the sub‐optimum level (S), and the second 32% DP, termed the optimum level (O), was fed in triplicate ponds. The weight gain of stocked prawn was significantly higher for G stock (3.03 ± 0.18 g) compared to M stock (2.53 ± 0.25 g) and A stock (1.33 ± 0.10 g). In addition weight gain in ODP was significantly higher (3.05 ± 0.22 g) compared to SDP (2.11 ± 0.17 g). Furthermore, both protein level and stock type had a significant (p ≤ 0.05) effect on the survival rate, specific growth rate, and feed conversion ratio of the prawn. A significant interaction was recorded between metabolic enzyme activities and the variation of dietary protein levels and differences in M. rosenbergii stocks, in which stock “G” and “M” exhibited healthier enzyme physiological status than stock “A”. The findings presented here may conclude that the enzymes quickly respond to dietary protein manipulations of cultured shrimp. The results can be useful in identifying the optimum stock (genotypes) in a given culture condition and subsequently higher production may be achieved.

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“…In vitro experiments on muscle and hepatopancreas of N. granulata showed the occurrence of different adjustments in metabolic pathways (i.e. changes in the carbon amino acid flux between gluconeogenesis and lipid synthesis) in response to hypo and hyper-osmotic stress (Martins et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Hyper-osmoregulation requires the involvement of various branchial ion transport mechanisms such as Na + /K + -ATPase activity and carbonic anhydrase, although the extent, timing and activity in individual gills can be speciesspecific; hypo-osmoregulation responses appear more variable and may require different mechanisms (López Mañanes et al 2000, 2002, Schleich et al 2001, Bianchini et al 2008, Freire et al 2008, McNamara and Faria 2012, Romano and Zeng 2012. Biochemical adaptation to environmental salinity appears to be a complex process involving also the participation of enzymes and transport systems in extrabranchial tissues (Jahn et al 2006, Pinoni and López Mañanes, 2004, 2009, Martins et al 2011. The biochemical changes occurring in salinity adaptation require a metabolic reorganization to support increasing energy demands (Freire et al 2008, McNamara and Faria 2012, Romano and Zeng 2012.…”

Section: Introductionmentioning

confidence: 99%

Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab <i>Cyrtograpsus angulatus</i> (Decapoda: Brachyura: Varunidae)

Michiels¹,

Valle²,

Mañanes³

2012

Sci. Mar.

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SUMMARY:We studied the occurrence and characteristics of lipase activity and the response of lipase and proteolytic activity to salinity and dopamine injections in hepatopancreas of the euryhaline crab (Cyrtograpsus angulatus). Lipase activity was maximal at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent K m =0.019 mM), was higher at 37°C and appeared to be cold tolerant, being also high at 4°C. In 10 psu (hyper-regulation conditions), lipase and proteolytic activity were about 3 and 5 times higher, respectively, than in 35 psu (osmoconformation). In 40 psu (hypo-regulation), lipase activity was about three times higher than in 35 psu, while proteolytic activity was similar. Lipase activity was inhibited in vivo by 10 -4 M dopamine in 35 psu but not in 10 or 40 psu. Proteolytic activity was not affected by 10 -4 M dopamine. The differential responses of lipase and proteolytic activity to salinity and dopamine suggest the occurrence of distinct digestive adjustments and mechanisms of regulation upon osmoregulatory conditions. This study contributes to a better understanding of the complexity of the biochemical adaptations to salinity in euryhaline crabs. The fact that higher digestive enzyme activities could be associated with a differential digestive capacity potentially leading to enhanced availability of energy substrates is discussed.Keywords: Cyrtograpsus angulatus, euryhaline crabs, salinity, hepatopancreas, digestive enzymes, dopamine, Mar Chiquita lagoon. RESUMEN: EfEcto dE la salinidad ambiEntal y dE la inyEcción dE dopamina sobrE Enzimas digEstivas clavE En hEpatopáncrEas dEl cangrEjo EurihalinoCyrtograpsus angulatus (dEcapoda: brachyura: varunidaE). -Se estudió la existencia y características bioquímicas de actividad de lipasa y la respuesta a salinidad y a dopamina de actividad de lipasa y proteolítica total en el hepatopáncreas del cangrejo eurihalino Cyrtograpsus angulatus. La actividad de lipasa fue máxima a pH 8,5; mostró una cinética michaeliana (K m =0.019 mM); fue mayor a 37°C, y resistente a baja temperatura (4°C). En 10 psu (condición de hiperregulación), las actividades de lipasa y proteolítica fueron 3 y 5 veces mayores, respectivamente, que en 35 psu (condición de osmoconformación). En 40 psu (condición de hiporregulación), la actividad de lipasa fue de alrededor 3 veces mayor que en individuos aclimatados a 35 psu, mientras que la actividad proteolítica fue similar. La actividad de lipasa fue inhibida in vivo por dopamina 10 -4 M en 35 psu pero no fue afectada en 10 o 40 psu. Dopamina 10 -4 M no afectó la actividad proteolítica. Las respuestas de las actividades de lipasa y proteolítica a salinidad y a dopamina permiten sugerir la existencia de ajustes digestivos y mecanismos de regulación diferenciales en relación al estado osmorregulatorio. Los resultados constituyen un aporte relevante al conocimiento sobre las complejas adaptaciones a nivel bioquímico a la salinidad en cangrejos eurihalinos.

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Effects of hypo- or hyperosmotic stress on lipid synthesis and gluconeogenic activity in tissues of the crab Neohelice granulata

Cited by 28 publications

References 31 publications

Effects of both ecdysone and the acclimation to low temperature, on growth and metabolic rate of juvenile freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae)

Effects of both ecdysone and the acclimation to low temperature, on growth and metabolic rate of juvenile freshwater crayfish Cherax quadricarinatus (Decapoda, Parastacidae)

Effect of different stock types and dietary protein levels on key enzyme activities of glycolysis‐gluconeogenesis, the pentose phosphate pathway and amino acid metabolism inMacrobrachiumrosenbergii

Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab <i>Cyrtograpsus angulatus</i> (Decapoda: Brachyura: Varunidae)

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