Effects of Angelica Extract on Schwann Cell Proliferation and Expressions of Related Proteins

Abstract

The present study investigated the effects of Angelica extract (AE) on Schwann cell proliferation and expressions of related proteins, including brain derived neurotrophic factor (BDNF), neural cell adhesion molecule (NCAM), and proliferating cell nuclear antigen (PCNA). Proliferation activity and cell cycles of SCs were evaluated by MTT assay and flow cytometry methods, respectively, after 12 h treatment of AE at different concentrations (62.5, 125, 250, 1000, 2000, 4000, and 8000 mg/L). SCs were treated by 500, 1000, and 2000 mg/L AE for 24 h or 48 h; the related genes mRNA and proteins expressions in SCs were detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) kit. At the concentration range of 125–2000 mg/L, the SC proliferation was induced by AE in a dose-dependent manner, especially 1000 and 2000 mg/L; cells in drug-treated groups showed the most increase. Cells counts were ascended significantly in (G2/M + S) phase compared to control group. BDNF, NCAM, and PCNA protein expressions significantly increased at drug-treated groups. Relative genes mRNA expressions levels were also significantly higher compared to control group. The results indicated that AE facilitated SC proliferation and related genes and proteins expressions, which provided a basic guideline for nerve injury repair in clinic.

Treadmill exercise activates ATF3 and ERK1/2 downstream molecules to facilitate axonal regrowth after sciatic nerve injury

“…Schwann cell proliferation after SNI is regulated by genes asso-ciated with the cell cycle and cell division, and these cell biological changes facilitate axonal regeneration of the injured peripheral nerves (Jiang et al, 2017). In previous studies on cell proliferation and nerve regeneration, cell division cycle-2 (Cdc2 or Cdk1) promoting G2-M phase transition in the cell cycle has been demonstrated to enhance axonal elongation of the injured sciatic nerve by activating Schwann cell proliferation and neurotrophic factors including brain-derived neurotrophic factor (Han et al, 2007) as well as ERK1/2 are reported to be activated by SNI to enhance Schwann cell proliferation (Seo et al, 2009).…”