Effects of <i>FABP4</i> variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

Li, Yunhai; Zhou, Huitong; Cheng, Long; Hodge, Miriam; Zhao, Jenny H.; Tung, Rosalind; Edwards, Grant; Hickford, Jonathan G. H.

doi:10.1017/s0022029920000011

Cited by 3 publications

(6 citation statements)

References 15 publications

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“…Bionaz and Loor (2008) described gene networks involved in bovine milk fat synthesis and suggested that the C14:0 FA in milk was mainly derived from de-novo synthesis in the mammary gland. Previous studies suggest that DGAT1, FABP4 and SCD could affect C14:0 FA levels (Li et al, 2020a(Li et al, , 2020b(Li et al, , 2021, and this was confirmed by the results in Supplementary Table S2, but no effect was observed for C14:0. After correcting for possible interactions with these genes, the effect of variant c.*302T>C on C10:0, C11:0, C12:0, C13:0 and C16:0 FA levels, and the MCFA and LCFA groups appeared to be stronger (i.e.…”

Section: Discussionsupporting

confidence: 81%

“…The cows were typed for DGAT1 , FABP4 and SCD1 variation using the methods described by Li et al . (2020 a , 2020 b , 2021) respectively. Only 405 of the original 409 cows could be typed for all three of these genes.…”

Section: Methodsmentioning

confidence: 99%

“…Interactions between different genes might be expected. The effects of DGAT1 p.K232A, variation in FABP4 and variation in SCD p.A293V on milk fat composition in these dairy cows have been described in previous studies (Li et al ., 2020 a , 2020 b , 2021). To correct for the potentially confounding effects of these genes, another GLMM (fixed effect: genotype, DIM, age, herd, DGAT1 p.K232A genotype, FABP4 genotype and SCD1 p.A293V genotype) and multiple pair-wise comparisons with Bonferroni corrections were used to ascertain the effect of genotypes with a frequency >5% (thus insuring adequate sample size) on milk FA traits.…”

Section: Methodsmentioning

confidence: 99%

“…After denaturation at 95°C for 5 min and rapid cooling on wet ice, the samples were loaded on 16 cm × 18 cm, different percentage acrylamide: bisacrylamide (37.5: 1) (Bio-Rad) gels and electrophoresis was performed using Protean II xi cells (Bio-Rad) at different conditions in 0.5× TBE buffer (Table 1), and gels were silver-stained using the method of Byun et al (2009). The cows were typed for DGAT1, FABP4 and SCD1 variation using the methods described by Li et al (2020aLi et al ( , 2020bLi et al ( , 2021 respectively. Only 405 of the original 409 cows could be typed for all three of these genes.…”

Section: Pcr-sscp Analysis and Genotypingmentioning

confidence: 99%

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Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows

Zhou

Cheng

et al. 2020

J. Agric. Sci.

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The current study investigated associations between variation in the bovine perilipin-2 gene (PLIN2) and milk traits (milk fat content, milk protein content, milk yield and milk fatty acid (FA) component levels) in 409 New Zealand pasture-grazed Holstein-Friesian × Jersey-cross (HF × J-cross or Kiwicross™) cows. Five nucleotide sequence variants were found in three regions of the gene, including c.17C>T in exon 2, c.53A>G in exon 3, c.595+23G>A and c.595+104_595+108del in intron 5, and c.*302T>C in the 3′-untranslated region. The c.*302T>C substitution produces two nucleotide sequence variants (A5 and B5), and this variation was associated with variation in milk protein content and milkfat composition for C10:0, C11:0, C12:0, C13:0 and C16:0 FA and medium-chain fatty acid (MCFA) and long-chain fatty acid (LCFA) groups. After correcting for the effect of variation in the diacylglycerol acyl-CoA acyltransferase 1 gene (DGAT1) that results in the amino acid substitution p.K232A, variation in the FA binding protein 4 gene (FABP4) and variation in the stearoyl-CoA desaturase (Δ-9-desaturase) gene (SCD) that results in the amino acids substitution p.A293V, significant differences between A5A5 and B5B5 cows were found for C10:0, C11:0, C12:0, C13:0, C16:0, and the MCFA, LCFA, total saturated FA and C10:1 index groups. This suggests that nucleotide sequence variation in PLIN2 may be affecting milk FA component levels.

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Section: Discussionsupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Pcr-sscp Analysis and Genotypingmentioning

confidence: 99%

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Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows

Zhou

Cheng

et al. 2020

J. Agric. Sci.

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“…Thus, our vascular proteome changes as we age. Here, we discuss one specific protein, which is equally important for meat quality [6], feed efficiency [7], and milk content variability in livestock [8,9] as it is for lipid metabolism in health and cardiovascular disease (CVD) in humans: fatty acid-binding protein 4 (FABP4). In this review, we will provide an update on some biology of FABP4, the role of FABP4 in atherosclerotic disease, genetic factors modifying FABP4 levels, drug development, and a future perspective.…”

Section: Introductionmentioning

confidence: 99%

The Effects of FABP4 on Cardiovascular Disease in the Aging Population

van der Ark-Vonk,

Puijk,

Pasterkamp

et al. 2024

Curr Atheroscler Rep

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Purpose of Review Fatty acid-binding protein 4 (FABP4) plays a role in lipid metabolism and cardiovascular health. In this paper, we cover FABP4 biology, its implications in atherosclerosis from observational studies, genetic factors affecting FABP4 serum levels, and ongoing drug development to target FABP4 and offer insights into future FABP4 research. Recent Findings FABP4 impacts cells through JAK2/STAT2 and c-kit pathways, increasing inflammatory and adhesion-related proteins. In addition, FABP4 induces angiogenesis and vascular smooth muscle cell proliferation and migration. FABP4 is established as a reliable predictive biomarker for cardiovascular disease in specific at-risk groups. Genetic studies robustly link PPARG and FABP4 variants to FABP4 serum levels. Considering the potential effects on atherosclerotic lesion development, drug discovery programs have been initiated in search for potent inhibitors of FABP4. Summary Elevated FABP4 levels indicate an increased cardiovascular risk and is causally related to acceleration of atherosclerotic disease, However, clinical trials for FABP4 inhibition are lacking, possibly due to concerns about available compounds’ side effects. Further research on FABP4 genetics and its putative causal role in cardiovascular disease is needed, particularly in aging subgroups.

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Overview of SNPs Associated with Trans Fat Content in Cow’s Milk

2023

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Lipids consumed with milk derivatives are one of the main parts of the human diet. Trans fatty acids in milk are causing a debate about their impact on the incidence of cardiovascular disease, pathological abnormalities, and cancer. The fatty acid profile of milk is influenced by a large number of different factors, one of which is genetic. The development of genetic studies, including Genome-Wide Association Studies (GWAS), may help define genomic regions associated with fatty acid content in milk, including trans fatty acids. This article provides an overview of international studies on the identification of genomic regions and SNPs associated with the trans fatty acids in cow’s milk. The results are based on research of cattle such as Norwegian Red cattle, Holstein, Jersey, and Brown Swiss. The presented review shows that 68 SNPs were localized on chromosomes 1, 2, 4–6, 8–10, 12, 14–20, 22–25, and 27–29. Further research in this direction will provide new information that will serve as an impetus for the creation of modern breeding technologies and increase the performance of the manufacture of high-quality dairy products. The search for genetic markers associated with the content of TFA in milk is a promising direction in agricultural science and will allow more complete breeding work with cattle.

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Effects of FABP4 variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

Cited by 3 publications

References 15 publications

Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows

Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows

The Effects of FABP4 on Cardiovascular Disease in the Aging Population

Overview of SNPs Associated with Trans Fat Content in Cow’s Milk

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