Effects of <i>galU</i> Mutation on <i>Pseudomonas syringae</i>–Plant Interactions

Deng, Wen-Ling; Lin, Yuan; Lin, Rong‐Hwa; Wei, Chia Fong; Huang, Yi Chiao; Peng, Hwei-Ling; Huang, Hsiou Chen

doi:10.1094/mpmi-23-9-1184

Cited by 26 publications

(22 citation statements)

References 55 publications

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“…Moreover, in Pseudomonas syringae this gene may change pathogen-host interaction, inducing PTI in tomato and preventing the survival of the pathogen inside the plant [77]. This is consistent with our finding that this protein is up-regulated in infectious conditions.…”

Section: Resultssupporting

confidence: 90%

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

et al. 2017

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Background Xanthomonas citri subsp. citri (Xac) is the causal agent of citrus canker. A proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of Xac during infection.ResultsFor that, a 2D–based proteomic analysis of Xac at 1, 3 and 5 days after inoculation, in comparison to Xac growth in NB media was carried out and followed by MALDI-TOF-TOF identification of 124 unique differentially abundant proteins. Among them, 79 correspond to up-regulated proteins in at least one of the three stages of infection. Our results indicate an important role of proteins related to biofilm synthesis, lipopolysaccharides biosynthesis, and iron uptake and metabolism as possible modulators of plant innate immunity, and revealed an intricate network of proteins involved in reactive oxygen species adaptation during Plants` Oxidative Burst response. We also identified proteins previously unknown to be involved in Xac-Citrus interaction, including the hypothetical protein XAC3981. A mutant strain for this gene has proved to be non-pathogenic in respect to classical symptoms of citrus canker induced in compatible plants.ConclusionsThis is the first time that a protein repertoire is shown to be active and working in an integrated manner during the infection process in a compatible host, pointing to an elaborate mechanism for adaptation of Xac once inside the plant.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1063-x) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 90%

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

et al. 2017

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“…LPS has also been shown to be an important virulence factor in various plant pathogenic bacteria including several Xanthomonas spp. [8], Erwinia amylovora [50] and Pseudomonas syringae [51]. It can serve as a physical barrier protecting bacteria from plant defense responses [51].…”

Section: Discussionmentioning

confidence: 99%

“…[8], Erwinia amylovora [50] and Pseudomonas syringae [51]. It can serve as a physical barrier protecting bacteria from plant defense responses [51]. It may also contribute to biofilm formation [23,24].…”

Section: Discussionmentioning

confidence: 99%

The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri

Wang

2012

BMC Microbiol

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BackgroundThe Gram-negative bacterium Xanthomonas citri subsp. citri (Xac) causes citrus canker, one of the most destructive diseases of citrus worldwide. In our previous work, a transposon mutant of Xac strain 306 with an insertion in the XAC3110 locus was isolated in a screening that aimed at identifying genes related to biofilm formation. The XAC3110 locus was named as bdp24 for biofilm-defective phenotype and the mutant was observed to be affected in extracellular polysaccharide (EPS) and lipopolysaccharide (LPS) biosynthesis and cell motility. In this study, we further characterized the bdp24 (XAC3110) gene (designated as gpsX) using genetic complementation assays and expanded the knowledge about the function of the gpsX gene in Xac pathogenesis by investigating the roles of gpsX in EPS and LPS production, cell motility, biofilm formation on host leaves, stress tolerance, growth in planta, and host virulence of the citrus canker bacterium.ResultsThe gpsX gene encodes a putative glycosyltransferase, which is highly conserved in the sequenced strains of Xanthomonas. Mutation of gpsX resulted in a significant reduction of the amount of EPS and loss of two LPS bands visualized on sodium dodecylsulphate- polyacrylamide gels. Biofilm assays revealed that the gpsX mutation affected biofilm formation by Xac on abiotic and biotic surfaces. The gpsX mutant showed delayed bacterial growth and caused reduced development of disease symptoms in susceptible citrus leaves. The gpsX mutant was more sensitive than the wild-type strain to various stresses, including the H2O2 oxidative stress. The mutant also showed attenuated ability in cell motility but not in flagellar formation. Quantitative reverse transcription-PCR assays indicated that mutation of gpsX did not affect the expression of virulence genes such as pthA in Xac strain 306. The affected phenotypes of the gpsX mutant could be complemented to wild-type levels by the intact gpsX gene.ConclusionsTaken together, our data confirm that the gpsX gene is involved in EPS and LPS synthesis and biofilm formation in Xac and suggest that the gpsX gene contributes to the adaptation of Xac to the host microenvironments at early stage of infection and thus is required for full virulence on host plants.

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“…These phenomena coincide with the normal stages of biofilm formation. Various mutations related to LPS synthesis in phytopathogenic bacteria such as P. aeruginosa [51], Pseudomonas syringae [52], Xanthomonas axonopodis [53], and Xanthomonas citri [54] caused reductions in both biofilm formation ability and virulence.…”

Section: Cell–cell and Cell–surface Interactions: Bacterial Biofilmentioning

confidence: 99%

The Role of Bacterial Biofilms and Surface Components in Plant-Bacterial Associations

Bogino

Oliva

Sorroche

et al. 2013

IJMS

406

243

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The role of bacterial surface components in combination with bacterial functional signals in the process of biofilm formation has been increasingly studied in recent years. Plants support a diverse array of bacteria on or in their roots, transport vessels, stems, and leaves. These plant-associated bacteria have important effects on plant health and productivity. Biofilm formation on plants is associated with symbiotic and pathogenic responses, but how plants regulate such associations is unclear. Certain bacteria in biofilm matrices have been found to induce plant growth and to protect plants from phytopathogens (a process termed biocontrol), whereas others are involved in pathogenesis. In this review, we systematically describe the various components and mechanisms involved in bacterial biofilm formation and attachment to plant surfaces and the relationships of these mechanisms to bacterial activity and survival.

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Effects of galU Mutation on Pseudomonas syringae–Plant Interactions

Cited by 26 publications

References 55 publications

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection

The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri

The Role of Bacterial Biofilms and Surface Components in Plant-Bacterial Associations

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