Effects of <i>in</i> vitro Culture Period of Reconstructed Embryos and Genetic Background of Feeder Cells on Establishment of Embryonic Stem Cells Derived from Somatic Cell Nuclear Transfer Blastocysts in Pigs

Han, Ning; Baek, Song Ee; Lee, Yongjin; Lee, Joohyeong; Yun, Jong-Il; Lee, Eunsong; Lee, Seung Tae

doi:10.12750/jarb.35.1.86

Cited by 4 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pigs (sus scrofa) are the most important animals in the field of livestock industry, toxicology, preclinical study, and biotechnology in Korea (Choi et al, 2020;Han et al, 2020;Kim et al, 2021). In normal and non-pregnant condition, the estrus cycle in pubertal female pigs repeats every 21 days with morphological changes in the ovaries Selection of suitable reference gene for gene expression studies of porcine ovaries under different conditions in quantitative reverse transcription polymerase chain reaction assay such as developing follicles to ovulation with luteolysis during the follicular phase (proestrus and estrus period for approximately 6 days) and small follicle waves with mature corpus luteum (CL) in the luteal phase (metestrus and diestrus period for approximately 15 days) by a complex regulatory network of excitatory and inhibitory hormones from the hypothalamic-pituitary-gonadal axis (Yang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Selection of suitable reference gene for gene expression studies of porcine ovaries under different conditions in quantitative reverse transcription polymerase chain reaction assay

Kim

Jeon

Jang

et al. 2022

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

The ovary undergoes substantial physiological changes along with estrus phase to mediate negative/positive feedback to the upstream reproductive tissues and to play a role in producing a fertilizable oocyte in the developing follicles. However, the disorder of estrus cycle in female can lead to diseases, such as cystic ovary which is directly associated with decline of overall reproductive performance. In gene expression studies of ovaries, quantitative reverse transcription polymerase chain reaction (qPCR) assay has been widely applied. During this assay, although normalization of target genes against reference genes (RGs) has been indispensably conducted, the expression of RGs is also variable in each experimental condition which can result in false conclusion. Because the understanding for stable RG in porcine ovaries was still limited, we attempted to assess the stability of RGs from the pool of ten commonly used RGs (18S, B2M, PPIA, RPL4, SDHA, ACTB, GAPDH, HPRT1, YWHAZ, and TBP) in the porcine ovaries under different estrus phase (follicular and luteal phase) and cystic condition, using stable RG-finding programs (geNorm, Normfinder, and BestKeeper). The significant (p < 0.01) differences in Ct values of RGs in the porcine ovaries under different conditions were identified. In assessing the stability of RGs, three programs comprehensively agreed that TBP and YWHAZ were suitable RGs to study porcine ovaries under different conditions but ACTB and GAPDH were inappropriate RGs in this experimental condition. We hope that these results contribute to plan the experiment design in the field of reproductive physiology in pigs as reference data.

show abstract

Section: Introductionmentioning

confidence: 99%

Selection of suitable reference gene for gene expression studies of porcine ovaries under different conditions in quantitative reverse transcription polymerase chain reaction assay

Kim

Jeon

Jang

et al. 2022

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

show abstract

“…Pigs (sus scrofa) are one of the most economically important livestock in the animal industry and important models in the field of the toxicology, preclinical study, and biotechnology due to strong similarities with the human (Ventrella et al, 2016;Choi et al, 2020;Han et al, 2020). As the animal industry in Korea, it is aggregated that the total number of 10,513,803 pigs has been raised in 4,406 domestic pig farms in Korea at the end of 2017 (available at https://kosis.kr).…”

Section: Introductionmentioning

confidence: 99%

Establishment of normal reference intervals in serum biochemical parameters of domestic sows in Korea

Kim

Son

et al. 2021

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

Because sows are industrially vital for swine production, monitoring for their health or disorder status is important to ensure high reproductive performance. Especially, ambient temperature changes in different season, especially during summer, are directly influenced to the reproductive performance of sows. Although the serum biochemical parameters are widely applied in the veterinary medicine with wide ranges for the physiological process, the values are also influenced by several factors such as age, breed, gender, and stress. In addition, domestic sows in Koreaspecific reference interval (RI) for serum biochemistry has not been established yet. Therefore, the present study was aimed to evaluate seasonal variation of RIs in the serum biochemistry in domestic sows in Korea at different seasons and to establish normal RIs using a RI finding program (Reference Value Advisor). Significant difference (p < 0.05) on the different seasons were identified in several serum biochemical parameters including BUN, CRE, GGT, GLU, ALB, TP, LDH and Na in sows. Therefore, we further established RIs, specific in domestic sows in Korea regardless of season. The established RIs based on the serum biochemical values provide a baseline for interpreting biochemical results in the domestic sows in Korea, regardless of seasonal effect. It may contribute to develop a strategy for better reproductive performance by improving breeding management practice and evaluating health of pig herds, which facilitate to avert the economic loss in summer infertility in sows.

show abstract

“…DNA-methylation is dynamic within the developmental stages, and the overall pattern is different between mouse and human [ 15 , 16 ]. Pigs have considerable physiological similarity to humans; thus, pigs can be used as a model to study early development [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos

Lee

Choe

et al. 2020

IJMS

View full text Add to dashboard Cite

Specification of embryonic lineages is an important question in the field of early development. Numerous studies analyzed the expression patterns of the candidate transcripts and proteins in humans and mice and clearly determined the markers of each lineage. To overcome the limitations of human and mouse embryos, the expression of the marker transcripts in each cell has been investigated using in vivo embryos in pigs. In vitro produced embryos are more accessible, can be rapidly processed with low cost. Therefore, we analyzed the characteristics of lineage markers and the effects of the DAB2 gene (trophectoderm marker) in in vitro fertilized porcine embryos. We investigated the expression levels of the marker genes during embryonic stages and distribution of the marker proteins was assayed in day 7 blastocysts. Then, the shRNA vectors were injected into the fertilized embryos and the differences in the marker transcripts were analyzed. Marker transcripts showed diverse patterns of expression, and each embryonic lineage could be identified with localization of marker proteins. In DAB2-shRNA vectors injected embryos, HNF4A and PDGFRA were upregulated. DAB2 protein level was lower in shRNA-injected embryos without significant differences. Our results will contribute to understanding of the mechanisms of embryonic lineage specification in pigs.

show abstract

Effects of in vitro Culture Period of Reconstructed Embryos and Genetic Background of Feeder Cells on Establishment of Embryonic Stem Cells Derived from Somatic Cell Nuclear Transfer Blastocysts in Pigs

Abstract: The pigs are considered as useful biomedical models and embryonic stem cells (ESCs) can self-renew and differentiate into three germ layers (Cha et al., 2018; Zhang et al., 2019). Therefore, ESCs derived from the inner cell

Cited by 4 publications

References 24 publications

Selection of suitable reference gene for gene expression studies of porcine ovaries under different conditions in quantitative reverse transcription polymerase chain reaction assay

Selection of suitable reference gene for gene expression studies of porcine ovaries under different conditions in quantitative reverse transcription polymerase chain reaction assay

Establishment of normal reference intervals in serum biochemical parameters of domestic sows in Korea

Identification of the Lineage Markers and Inhibition of DAB2 in In Vitro Fertilized Porcine Embryos

Contact Info

Product

Resources

About