Effects of Juglans regia Root Bark Extract on Platelet Aggregation, Bleeding Time, and Plasmatic Coagulation: In Vitro and Ex Vivo Experiments

Abstract

Platelets have an important role in thrombosis and haemostasis. Hyperactivity of the platelets has been associated with thromboembolic diseases and represents the main cause of complications of cardiovascular diseases. Crude aqueous extract (CAE) of Juglans regia root bark was evaluated for bleeding time, antiaggregant activity by using agonists, thrombin, ADP, collagen, or arachidonic acid (in vitro and ex vivo), and anticoagulant activity by measuring the clotting parameters: activated partial thromboplastin time, prothrombin time, thrombin time, and fibrinogen dosage (in vitro and ex vivo). The result of this study reported that the strongest antiaggregant effect of CAE in vitro was observed on the ADP-induced aggregation with inhibitions up to 90 %, while, in ex vivo experiments, the inhibition (more than 80 %) was observed with all agonists. Anticoagulant effect of CAE significantly prolonged the TT and decreased the fibrinogen level in vitro and ex vivo without interfering with APTT and PT. The bleeding time in mice and rats was significantly increased by CAE. The antiplatelet and anticoagulant effect observed in this study suggest that Juglans regia could have antithrombotic and/or thrombolytic activities and provide an alternative therapy against thrombotic complications related to cardiovascular diseases.

Antithrombotic, Antiaggregant and Anticoagulant Effect of Methanolic Fraction of Calamintha officinalis: In vitro and ex vivo Experiments

“…The current results indicated that MFCO prolonged significantly the bleeding time in mice and rats. As reported in previous similar studies, the inhibition of platelet aggregation can be associated to a prolongation of bleeding time 18,19 . However, some other studies noted no change in bleeding time when platelet aggregation was suppressed 20,21 .…”

Chemical profile of Tetraclinis articulata (Vahl) Masters, and Juglans regia L. and Olea europaea L. var. Sylvestris used against oral diseases: in vitro analysis between polyphenolic content and aqueous extraction optimization

“…Walnut yields were less than some aqueous extracts of 5 cultivars ( Juglans regia L.) green husks which are very similar in different varieties of walnut, from 31.63 to 33.69% ( Oliveira et al., 2008 ), but it was higher than Leaf yield of ethanol:water (4:6) extract which was 20.1% ( Almeida et al., 2008 ) and bark yield of infused extract 11.8 % ( Amirou et al., 2018 ).…”

Natural aldose reductase inhibitors for treatment and prevention of diabetic cataract: A review

“…Juglans regia L. is a medicinal plant that possesses several pharmacological effects. Its root had antiplatelet and anticoagulant effects [80]. Furthermore, its leaves have antioxidant, anti-inflammatory, anticancer, antihyperglycemic actions, and decrease levels of serum triglyceride, cholesterol and blood urea nitrogen in rats [81].…”