Effects of Lonicera caerulea extract on adipocyte differentiation and adipogenesis in 3T3-L1 cells and mouse adipose-derived stem cells (MADSCs)

Abstract

Purpose: Obesity is a major health problem of global significance because it is clearly associated with an increased risk of health problems, such as nonalcoholic fatty liver disease (NAFLD), diabetes, cardiovascular diseases, and cancer. Lonicera caerulea (LC) originates from high mountains or wet areas and has been used as a traditional medicine in northern Russia, China, and Japan. LC contains a range of bioactive constituents, such as vitamins, minerals, and polyphenols. This study examined the anti-obesity effects of LC during differentiation in preadipocytes. Methods: The cell viability assay was performed after the differentiation of 3T3-L1 cells for 7 days. Oil Red O staining was used to visualize the changes in lipid droplets in 3T3-L1 cells and mouse adipose-derived stem cells (MADSCs). The mRNA expression of obesity-related genes was determined by quantitative real-time PCR. Results: According to the results of Oil Red O staining, the lipid levels and size of lipid droplets in the adipocytes were reduced and the LC extract (LCE, 0.25-1 mg/mL) markedly inhibited adipogenesis in a dose-dependent manner. The treatment of LCE also decreased the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα), and sterol regulatory element binding protein 1 (SREBP1) in 3T3-L1 cells. Western blot analysis showed that the PPARγ, C/EBPα, and SREBP1 protein levels in both 3T3-L1 and MADSC were reduced in a dose-dependent manner. Conclusion: These results suggest that LCE can inhibit adipogenic differentiation through the regulation of adipogenesis-related markers.

Blue honeysuckle rich in cyanidin-3-O-glucoside inhibited adipogenic differentiation by modulation of the adipogenesis pathway in 3T3-L1 adipocytes

“…Another study found that the content of the anthocyanins from the BH ranged from 1.16 to 5.93 mg/g, and the C3G was the major one, which occupied 79-88% (10). The previous reports did not show a significant decrease on the cells viability in the BH treatment up to 1,000 μg/mL (19), which is similar to our results.…”

“…According to the previous reports, the triglyceride accumulation was reduced to 77.42% in the 1% BH supplemented group of the HFD-induced obese mice, but the results for the free glycerol study could not be found (19). The results from the current study indicated that the treatment of the BH did not induce the lipolysis, but it inhibited the adipogenesis.…”

“…A previous study reported that the BH reduced body fat, the abdominal fat density, the total cholesterol, and triglycerides under HFD mice (20). According to a recent study, the BH at 1 mg/mL reduced both adipocyte lipid contents up to 82% and the lipid droplet size (19). This study also confirmed that the BH suppressed adipogenesis by the down regulating of the transcription factors, such as PPARγ, C/EBPα, and SREBP1, so that key transcriptional genes would be influenced from the certain concentration of BH extract.…”