Effects of <i>Momordica</i> saponin extract on alleviating fat accumulation in <i>Caenorhabditis elegans</i>

Lin, Chunxiu; Lin, Yizi; Chen, Yue; Xu, Jiena; Li, Jun; Cao, Yong; Su, Zuanxian

doi:10.1039/c9fo00254e

Cited by 37 publications

(44 citation statements)

References 36 publications

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“…The PPAR may have different target genes dependent to co-factors, such as C/EBPs and PPAR coactivator 1α (PGC1α) ( Chung et al., 2016 ); PGC1α and C/EBPβ were both involved in the increased fatty acid oxidation by CPT1 in human nasopharyngeal cancer cells ( Du et al., 2019 ). Homologs of these co-factors are MDT-15/PGC1α and CEBP-2/C/EBP in C. elegans , respectively ( Lin et al., 2019 , Xu et al., 2015 ). However, neither MDT-15 nor CEBP-2 was identified as co-factors for the isorhamnetin's fat-lowering effects via NHR-49-mediated pathway in the current study, which also explain the null effect of isorhamnetin on cpt-5 /CPT1 expression.…”

Section: Discussionmentioning

confidence: 99%

“…The anti-obesity mechanisms of action of dietary bioactives and plant extracts on lipid metabolism have various molecular targets in C. elegans ( Farias-Pereira et al., 2018 , Lin et al., 2019 , Liu et al., 2018 , Machado et al., 2018 , Shen et al., 2017 , Shen et al., 2018c , Sun et al., 2016 , Yue et al., 2019 ). It was reported that the inhibitory effects of NHR-49 on fatty acid desaturases (lipogenesis) were related to the fat-lowering effects of a saponin extract in C. elegans ( Lin et al., 2019 ). AAK was found to be regulated for isorhamnetin and methylated resveratrol associated with fatty acid oxidation and lipogenesis, respectively ( Yue et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Human lipid metabolism pathways are conserved in C. elegans , which help to identify the mechanisms of action of food components prior to vertebrate animals and/or humans ( Shen et al., 2018a , Shen et al., 2018b ). Previously, studies have examined the anti-obesity effects of plant extracts and food bioactive components in C. elegans , showing the relevance of this animal model for the mechanistic studies of prospective bioactives ( Bhattacharya et al., 2013 , Farias-Pereira et al., 2018 , Farias-Pereira et al., 2020 , Lin et al., 2019 , Liu et al., 2018 , Machado et al., 2018 , Shen et al., 2017 , Shen et al., 2018a , Shen et al., 2018b , Shen et al., 2018c , Sun et al., 2016 , Yue et al., 2019 ). Thus, the goal of this study was to determine the effects of isorhamnetin on lipid metabolism by using C. elegans as an animal model.…”

Section: Introductionmentioning

confidence: 99%

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Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Farias-Pereira

Savarese

Yue

et al. 2020

Current Research in Food Science

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Isorhamnetin (3- O -methylquercetin), a flavonol found in dill weed, sea buckthorn berries, kale and onions, has been suggested to have anti-obesity effects, but there is limited evidence of its mechanisms of action on lipid metabolism. The goal of this study was to investigate the effects of isorhamnetin on lipid metabolism using Caenorhabditis elegans as an animal model. Isorhamnetin reduced fat accumulation without affecting food intake or energy expenditure in C. elegans . The isorhamnetin's fat-lowering effects were dependent on nhr-49 , a homolog of the human peroxisome proliferator-activated receptor alpha (PPARα). Isorhamnetin upregulated an enoyl-CoA hydratase ( ech-1.1 , involved in fatty acid β-oxidation) and adipose triglyceride lipase ( atgl-1 , involved in lipolysis) via NHR-49-dependent pathway at transcriptional levels. Isorhamnetin also upregulated the C. elegans AMP-activated protein kinase (AMPK) subunits homologs ( aak-1 and aak-2 ), involved in energy homeostasis. These results suggest that isorhamnetin reduces body fat by increasing fat oxidation in part via NHR-49/PPARα-dependent pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Farias-Pereira

Savarese

Yue

et al. 2020

Current Research in Food Science

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“…Regarding the saponin content, none of the triterpenoid saponins identified in CV have yet been investigated. However, an isolated triterpenoid saponin from Panax ginseng [ 95 ], a steroidal saponin from Asparagus racemosus [ 96 ], and plant extracts particularly rich in triterpenoid saponins [ 97 , 98 ] were previously reported to enhance stress resistance and to prolong lifespan in C. elegans . In the study of Magid et al [ 50 ], saponins accounted for only 0.5% of the total composition of a methanol extract of piquiá shells.…”

Section: Resultsmentioning

confidence: 99%

Piquiá Shells (Caryocar villosum): A Fruit by-Product with Antioxidant and Antiaging Properties in Caenorhabditis elegans

Roxo

Peixoto

Wetterauer

et al. 2020

Oxidative Medicine and Cellular Longevity

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In a context of rising demand for sustainable antiaging interventions, fruit processing by-products are a promising source of bioactive compounds for the production of antiaging dietary supplements. Piquiá (Caryocar villosum) is a native Amazonian fruit consisting of 65% nonedible shells. In the present study, the phytochemical profile of a hydroalcoholic extract of piquiá shells (CV) was characterized by LC-MS/MS analysis. Its antioxidant and antiaging activities were investigated using the nematode Caenorhabditis elegans as an in vivo model. CV is mainly composed by hydrolysable tannins and triterpenoid saponins. The extract enhanced stress resistance of wild-type and mutant worms by reducing the intracellular levels of reactive oxygen species (ROS) and by increasing their survival against a lethal dose of the prooxidant juglone. These effects involved the upregulation of sod-3 and downregulation of gst-4 and hsp-16.2, studied through the GFP fluorescent reporter intensity and at the transcriptional level by qRT-PCR analysis. CV extended the lifespan of wild-type worms in a DAF-16/FoxO- and SKN-1/Nrf-dependent manner. Taken together, our findings indicate piquiá shells as potential candidates for nutraceutical applications. Further studies are needed to validate the relevance of our findings to antiaging interventions in humans.

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“…11 Interestingly, we also noted the potential of CPPs and MSs in relieving fat deposition and improving resistance in C. elegans. [13][14][15][16] Therefore, MSs may be a dietary supplement to treat obesity and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Effect of Momordica saponin‑ and Cyclocarya paliurus polysaccharide‐enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans

Lin

Xiao

et al. 2020

J Sci Food Agric

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BACKGROUND: As an edible and medicinal herb in Chinese folk medicine, Cyclocarya paliurus (Batal.) Iljinskaja leaves are traditionally widely used in the treatment of metabolic disorders. The vegetable Momordica charantia L. has been consumed worldwide for thousands of years as a traditional drug due to its activities against obesity and diabetes. In view of the therapeutic value of Momordica saponins (MSs) and C. paliurus polysaccharides (CPPs), an independently developed MSs-and CPPs-containing beverage (MC) was evaluated for its efficacy in controlling oxidative stress and obesity in Caenorhabditis elegans.RESULTS: First, we found that MC could promote the nuclear localization of DAF-16 and the translation of SOD-3. Further exploring its antioxidant properties, the oxidative stress by-products reactive oxygen species, malondialdehyde, and nonesterified fatty acids were significantly inhibited in C. elegans. Moreover, damage due to diseases related to oxidative stress (age pigments and neurodegenerative diseases) was alleviated. Furthermore, fat accumulation was significantly reduced in normal and high-fat models. Finally, the lipid-lowering effects of MC might involve reductions in the size and number of lipid droplets without impairing basic physiological functions in C. elegans.CONCLUSION: These results provide promising data indicating MC as an innovative health beverage for the pharmacological management of oxidative stress and obesity.

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Effects of Momordica saponin extract on alleviating fat accumulation in Caenorhabditis elegans

Abstract: The study offers methods and models for elucidating fat accumulation and mechanisms, valuable for studies of other plant-based nutraceuticals.

Cited by 37 publications

References 36 publications

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Piquiá Shells (Caryocar villosum): A Fruit by-Product with Antioxidant and Antiaging Properties in Caenorhabditis elegans

Effect of Momordica saponin‑ and Cyclocarya paliurus polysaccharide‐enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans

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