Effects of<i>Ocimum</i>spp. essential oil on<i>Monilinia laxa in vitro</i>

Carović‐Stanko, Klaudija; Fruk, Goran; Šatović, Zlatko; Ivić, Dario; Politeo, Olivera; Sever, Zdravka; Grdiša, Martina; Strikić, Frane; Jemrić, Tomislav

doi:10.1080/10412905.2012.751057

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…Furthermore, the in vitro application of Ocimum basilicum var. purpurascens, Ocimum tenuiflorum, R. officinalis, and Thymbra spicata EOs was shown to have significant effects on the mycelial growth of M. laxa or M. fructigena, with inhibition exceeding 100% in the case of R. officinalis (16 µL/Petri dish) and T. spicata (2 µL/Petri dish) [55][56][57]. The same usage of thyme, savory, and oregano EOs on nectarine fruits, on the other hand, was found to be phytotoxic [55].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Antifungal Activities of Nine Commercial Essential Oils against Brown Rot in Apples

et al. 2021

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After harvest, numerous plant pathogenic fungi can infect fresh fruits during transit and storage. Although synthetic fungicides are often used to manage postharvest fruit diseases, their application may lead to problems such as the development of fungicide resistance and residues on fruits. In the present study, the antifungal potential of nine commercial essential oils (EOs) extracted from Eucalyptus radiata ssp. radiata, Mentha pulegium, Rosmarinus officinalis, Origanum compactum, Lavandula angustifolia, Syzygium aromaticum, Thymus vulgaris, Citrus aurantium, and Citrus sinensis were tested against the apple brown rot fungi Monilinia laxa and Monilinia fructigena at different concentrations in vitro (against mycelial growth and spore germination) and in vivo (on detached apple fruit and in semi-commercial postharvest conditions). In addition, fruit quality parameters were evaluated and the composition of the EOs was characterized by Fourier transform infrared (FT-IR) spectroscopy. In vitro results showed significant antifungal activity of all tested EOs on both fungal species. EOs from S. aromaticum were the most effective, whereby inhibition percentages ranged from 64.0 to 94.7% against M. laxa and from 63.9 to 94.4% against M. fructigena for the concentrations 12.5 and 100 µL/mL, respectively, with an EC50 of 6.74 µL/mL for M. laxa and 10.1 µL/mL for M. fructigena. The higher concentrations tested of S. aromaticum, T. vulgaris, C. aurantium, and C. sinensis EOs significantly reduced spore germination, brown rot incidence, and lesion diameter. Evaluation of the treatments during storage for 20 days at 4 °C on apple fruit quality parameters demonstrated the preservation of the fruit quality characteristics studied (weight loss, total soluble solids, titratable acidity, firmness, and maturity index). FT-IR spectra obtained from all tested EO samples presented characteristic peaks and a high diversity of functional groups such as O–H groups, C–H bonds, and C–C stretching. The EOs examined here may have the potential for controlling postharvest fungal diseases of fruit such as brown rot.

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Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Antifungal Activities of Nine Commercial Essential Oils against Brown Rot in Apples

et al. 2021

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“…Because it is exceptionally rich in essential oils, it is commonly produced for economic purposes ( Caliskan et al, 2017 ). It has a wide range of applications; it is used as a spice in many cuisines, as an ingredient for commercial fragrances, flavors, to improve the food products shelf life, in traditional medicine and phytotherapy ( Labra et al, 2004 ; Carović-Stanko et al, 2010 ; Radácsi et al, 2010 ; Mishra et al, 2012 ; Carović-Stanko et al, 2013 ; Purushothaman et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.)

et al. 2021

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Basil is one of the most widespread aromatic and medicinal plants, which is often grown in drought- and salinity-prone regions. Often co-occurrence of drought and salinity stresses in agroecosystems and similarities of symptoms which they cause on plants complicates the differentiation among them. Development of automated phenotyping techniques with integrative and simultaneous quantification of multiple morphological and physiological traits enables early detection and quantification of different stresses on a whole plant basis. In this study, we have used different phenotyping techniques including chlorophyll fluorescence imaging, multispectral imaging, and 3D multispectral scanning, aiming to quantify changes in basil phenotypic traits under early and prolonged drought and salinity stress and to determine traits which could differentiate among drought and salinity stressed basil plants. Ocimum basilicum “Genovese” was grown in a growth chamber under well-watered control [45–50% volumetric water content (VWC)], moderate salinity stress (100 mM NaCl), severe salinity stress (200 mM NaCl), moderate drought stress (25–30% VWC), and severe drought stress (15–20% VWC). Phenotypic traits were measured for 3 weeks in 7-day intervals. Automated phenotyping techniques were able to detect basil responses to early and prolonged salinity and drought stress. In addition, several phenotypic traits were able to differentiate among salinity and drought. At early stages, low anthocyanin index (ARI), chlorophyll index (CHI), and hue (HUE2D), and higher reflectance in red (RRed), reflectance in green (RGreen), and leaf inclination (LINC) indicated drought stress. At later stress stages, maximum fluorescence (Fm), HUE2D, normalized difference vegetation index (NDVI), and LINC contribute the most to the differentiation among drought and non-stressed as well as among drought and salinity stressed plants. ARI and electron transport rate (ETR) were best for differentiation of salinity stressed plants from non-stressed plants both at early and prolonged stress.

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“…In vivo assays indicate that T. vulgaris and C. zeylanicum EO VOCs can also protect peaches from brown rot caused by M. laxa via the induction of physiological and defensive responses in the fruit ( Cindi et al., 2016 ; Khumalo et al., 2017 ). EO VOCs from different Ocimum basilicum varieties also demonstrated in vitro antifungal activity against this pathogen ( Carović-Stanco et al., 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…Inhibitory effects of some EOs volatiles against M. fructicola have been also reported, such as T. vulgaris ( Sellamuthu et al., 2013 ; Spadaro et al., 2021 ) and M. alternifolia ( Xu et al., 2022 ). O. vulgare ( Carović-Stanco et al., 2013 ) and T. vulgaris ( Khumalo et al., 2017 ; Pinto et al., 2020 ) EO VOCs also inhibited M. laxa growth. Nevertheless, there are many plant EOs that have not been tested yet in volatile phase against these major phytopathogenic fungi.…”

Section: Introductionmentioning

confidence: 96%

Antifungal activity of volatile organic compounds from essential oils against the postharvest pathogens Botrytis cinerea, Monilinia fructicola, Monilinia fructigena, and Monilinia laxa

Álvarez-García,

Moumni,

Romanazzi

2023

Front. Plant Sci.

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Gray mold and brown rot, caused respectively by Botrytis cinerea and Monilinia spp., are fungal diseases responsible for significant losses during the storage of fruit and vegetables. Nowadays, the control of postharvest diseases is shifting towards more sustainable strategies, including the use of plant secondary metabolites. In this study, the antifungal activity of Origanum vulgare, Thymus vulgaris, Thymus serpyllum, Melaleuca alternifolia, Lavandula officinalis, Lavandula hybrida, Citrus bergamia, Rosmarinus officinalis, Cinnamomum zeylanicum essential oils (EOs) in vapor phase was tested in vitro against B. cinerea, Monilinia fructicola, Monilinia fructigena, and Monilinia laxa. For the experiments, a protocol using a volatile organic compounds (VOC) chamber was designed. Results indicate a dose-dependent inhibitory activity of all the tested EOs, with O. vulgare, T. vulgaris, and T. serpyllum being the most active ones, with minimum inhibitory concentrations (MIC) of 22.73, 45.45, and 22.73 µl/L, respectively, against B. cinerea and a range between 5.64 and 22.73 µl/L against the three Monilinia spp. Overall, B. cinerea presented lower sensitivity to vapor-phase EOs than any of the Monilinia strains, except for the C. zeylanicum EO, which consistently showed higher inhibition against B. cinerea. Among the three Monilinia spp., M. fructicola was the least sensitive, while M. fructigena was the most sensitive. The use of VOC chambers proved to be a reliable protocol for the assessment of antimicrobial activities of EOs. These results suggest that the VOC emitted by the tested EOs are effective towards important decay-causing fungi, and that they could be used for the control of gray mold and brown rot in in vivo trials.

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Effects ofOcimumspp. essential oil onMonilinia laxa in vitro

Cited by 11 publications

References 24 publications

In Vitro and In Vivo Antifungal Activities of Nine Commercial Essential Oils against Brown Rot in Apples

In Vitro and In Vivo Antifungal Activities of Nine Commercial Essential Oils against Brown Rot in Apples

Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.)

Antifungal activity of volatile organic compounds from essential oils against the postharvest pathogens Botrytis cinerea, Monilinia fructicola, Monilinia fructigena, and Monilinia laxa

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