Effects of icariin on the proliferation and osteogenic differentiation of human amniotic mesenchymal stem cells

Abstract

Background Tissue engineering technology has been applied extensively for clinical research and human amnion mesenchymal stem cells (hAMSCs) could cause mesenchymal stem cells to differentiate into the bone tissue. However, it is necessary to develop and identify the safer appropriate amount of osteogenic inducer. The objective of this study is to investigate the effect of icariin (ICA) on the proliferation and osteogenic differentiation of hAMSCs. Methods The morphology and phenotype of hAMSCs were discovered by flow cytometry and immunocytochemical staining. The osteogenic differentiation of hAMSCs under the influence of different concentrations of ICA were assessed by alkaline phosphatase (ALP) activity substrate assay and alizarin red staining. Results MTT assay revealed that the hAMSCs pretreated with ICA exhibited increased proliferation when compared with the control group, and the most optimum concentration of ICA was 1 × 10− 6 mol/L. The combined analysis of ALP activity and ARS staining showed that ICA could significantly promote the osteogenic differentiation of hAMSCs, and the effect was most significant when the concentration of ICA was 1 × 10− 6 mol/L. Conclusion All the above results implied that ICA could significantly increase proliferation and enhance the osteogenic differentiation of hAMSCs, especially when the concentration of ICA was 1 × 10− 6 mol/L.

Icariin promotes the proliferation and osteogenic differentiation of bone-derived mesenchymal stem cells in patients with osteoporosis and T2DM by upregulating GLI-1

“…The present study found that ICA treatment induced significant increases in RUNX2, ALP, and SPP1 after 7-d induction, and an enhancement of mineralization level in the later stage (21-d induction), thus confirming a positive effect of ICA on the osteogenic differentiation of BMSCs. The ideal dose (5 μM) was different from those (1 or 20 μM) in previous studies [ 25 , 26 ], and this may be related to the properties of MSCs.…”

“…The ICA dose used in the cytotoxicity assay varied from 1 to 100 μM, which was based on several relevant studies. For instance, 1 and 10 μM ICA are both positive for the osteogenic potency of amnion MSC, and 1, 10, and 100 μM ICA are all able to promote the proliferation of amnion MSC [ 25 ]. Another study indicated that 20 μM ICA has a better osteogenic stimulating effect on bone marrow BMSC than doses of 10 or 40 μM [ 26 ].…”

Icariin-Loaded Hydrogel Regulates Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation and Promotes Cartilage Repair in Osteoarthritis

“…Icariin is one of the effective ingredients of traditional Chinese medicine Epimedium . Appropriate concentration of icariin has a stimulatory effect on the proliferation of MSCs and chondrocytes ( Ye et al, 2017 ; Liu et al, 2019 ; Wang et al, 2020 ). However, this inductive proliferation effect is related to the content of icariin in the hydrogels ( Jiao et al, 2018 ).…”

Calcium phosphate cement with icariin-loaded gelatin microspheres as a local drug delivery system for bone regeneration

“…It was observed the cells cultured with the leaching liquors of CPC/GMs(ICA) displayed slightly higher proliferation ability than CPC/GMs and the control groups, indicating that the cements were highly compatible and the cement with ICA-loaded GMs was able to increase BMSCs proliferation. Several studies showed that ICA signi cantly promoted the proliferation of BMSCs at a concentration range between 10 − 9 and 10 − 5 mol/L, although the optimal concentration remains uncertain [24,25] . It was reasonable to presume that the concentration of ICA loaded in the calcium phosphate cements was appropriate for further experiments.…”