Effects of Iliac Stenosis on Abdominal Aortic Aneurysm Formation in Mice and Humans

Sangha, Gurneet S.; Busch, Albert; Acuna, Andrea; Berman, Alycia G.; Phillips, Evan; Trenner, Matthias; Eckstein, H.‐H.; Maegdefessel, Lars; Goergen, Craig J.

doi:10.1159/000501312

Cited by 11 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, an uneven geometry of the aneurysm centerline might influence an uneven distribution of ILT affecting the histologic apparel and vice versa [ 3 , 30 ]. The five patients investigated here did not show excessive bulging to either side of their respective aneurysm (Fig.…”

Section: Discussionmentioning

confidence: 99%

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Menges,

Nackenhorst,

Müller

et al. 2023

Diagn Pathol

Self Cite

View full text Add to dashboard Cite

Abdominal aortic aneurysm (AAA) is a pathologic enlargement of the infrarenal aorta with an associated risk of rupture. However, the responsible mechanisms are only partially understood. Based on murine and human samples, a heterogeneous distribution of characteristic pathologic features across the aneurysm circumference is expected. Yet, complete histologic workup of the aneurysm sac is scarcely reported. Here, samples from five AAAs covering the complete circumference partially as aortic rings are investigated by histologic means (HE, EvG, immunohistochemistry) and a new method embedding the complete ring. Additionally, two different methods of serial histologic section alignment are applied to create a 3D view. The typical histopathologic features of AAA, elastic fiber degradation, matrix remodeling with collagen deposition, calcification, inflammatory cell infiltration and thrombus coverage were distributed without recognizable pattern across the aneurysm sac in all five patients. Analysis of digitally scanned entire aortic rings facilitates the visualization of these observations. Immunohistochemistry is feasible in such specimen, however, tricky due to tissue disintegration. 3D image stacks were created using open-source and non-generic software correcting for non-rigid warping between consecutive sections. Secondly, 3D image viewers allowed visualization of in-depth changes of the investigated pathologic hallmarks. In conclusion, this exploratory descriptive study demonstrates a heterogeneous histomorphology around the AAA circumference. Warranting an increased sample size, these results might need to be considered in future mechanistic research, especially in reference to intraluminal thrombus coverage. 3D histology of such circular specimen could be a valuable visualization tool for further analysis.

show abstract

Section: Discussionmentioning

confidence: 99%

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Menges,

Nackenhorst,

Müller

et al. 2023

Diagn Pathol

Self Cite

View full text Add to dashboard Cite

show abstract

“…While the grafts evaluated in this study did not reach the compliance of the native aorta, they developed to levels similar to those seen in aneurysmal murine arteries. [49][50][51] This may be due to the initial stiffness of the implanted scaffolds, thus a more compliant scaffold may have facilitated mechanical properties that better match the native vessel. Recall, also, that the more compliant scaffolds evaluated in this study, the 2 × 2 braided designs, were still significantly less compliant a than native vessel and had a high failure rate due to the lack of structural integrity, both initially and over the course of degradation.…”

Section: Discussionmentioning

confidence: 99%

Effects of Braiding Parameters on Tissue Engineered Vascular Graft Development

Zbinden

Blum

Berman

et al. 2020

Adv Healthcare Materials

View full text Add to dashboard Cite

Tissue engineered vascular grafts (TEVGs) using scaffolds fabricated from braided poly(glycolic acid) (PGA) fibers coated with poly(glycerol sebacate) (PGS) are developed. The approach relies on in vivo tissue engineering by which neotissue forms solely within the body after a scaffold has been implanted. Herein, the impact of altering scaffold braid design and scaffold coating on neotissue formation is investigated. Several combinations of braiding parameters are manufactured and evaluated in a Beige mouse model in the infrarenal abdominal aorta. Animals are followed with 4D ultrasound analysis, and 12 week explanted vessels are evaluated for biaxial mechanical properties as well as histological composition. Results show that scaffold parameters (i.e., braiding angle, braiding density, and presence of a PGS coating) have interdependent effects on the resulting graft performance, namely, alteration of these parameters influences levels of inflammation, extracellular matrix production, graft dilation, neovessel distensibility, and overall survival. Coupling carefully designed in vivo experimentation with regression analysis, critical relationships between the scaffold design and the resulting neotissue that enable induction of favorable cellular and extracellular composition in a controlled manner are uncovered. Such an approach provides a potential for fabricating scaffolds with a broad range of features and the potential to manufacture optimized TEVGs.

show abstract

“…Bowden et al ( 102 ) summarized the availability of in vitro and in vivo models to examine shear stress in AAA. Accordingly, porcine pancreatic elastase (PPE) infusion, along with partial ligation of the iliac artery in murine and rodent models, can increase mechanical stretch and shear stress and alter the morphology of AAA phenotypes ( 103 , 104 ). However, the difference in SS experienced between murine and human vasculature can lead to significant variation and bias in translating the results effectively.…”

Section: Mechanotransduction In Aaa: a Translational Perspectivementioning

confidence: 99%

Mechanosignals in abdominal aortic aneurysms

Lowis

Winaya

Kumari

et al. 2023

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Cumulative evidence has shown that mechanical and frictional forces exert distinct effects in the multi-cellular aortic layers and play a significant role in the development of abdominal aortic aneurysms (AAA). These mechanical cues collectively trigger signaling cascades relying on mechanosensory cellular hubs that regulate vascular remodeling programs leading to the exaggerated degradation of the extracellular matrix (ECM), culminating in lethal aortic rupture. In this review, we provide an update and summarize the current understanding of the mechanotransduction networks in different cell types during AAA development. We focus on different mechanosensors and stressors that accumulate in the AAA sac and the mechanotransduction cascades that contribute to inflammation, oxidative stress, remodeling, and ECM degradation. We provide perspectives on manipulating this mechano-machinery as a new direction for future research in AAA.

show abstract

Effects of Iliac Stenosis on Abdominal Aortic Aneurysm Formation in Mice and Humans

Cited by 11 publications

References 40 publications

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Completing the view – histologic insights from circular AAA specimen including 3D imaging

Effects of Braiding Parameters on Tissue Engineered Vascular Graft Development

Mechanosignals in abdominal aortic aneurysms

Contact Info

Product

Resources

About