Effects of immunopotentiators on biochemical parameters, proinflammatory cytokine, and nonspecific immune responses in Shaoxing ducklings

Gu, Tiantian; Li, Guoqin; Wu, Xinsheng; Zeng, Tao; Xu, Qi; Li, Liumeng; Vladyslav, Spyrydonov; Chen, Guohong; Liu, Lizhi

doi:10.1016/j.psj.2020.08.069

Cited by 15 publications

(6 citation statements)

References 32 publications

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“…Measuring cytokine production is an integral part of evaluating the cell-mediated immune response [ 33 ]. IL-1β, a major coordinator of the immune response, can stimulate immune cells to release a variety of cytokines [ 34 ]. TNF-α is a key cell signaling protein in the inflammatory response and induced apoptosis in a variety of cell types [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dietary Chlorogenic Acid on Growth Performance, Antioxidant Function, and Immune Response of Broiler Breeders under Immune Stress and Stocking Density Stress

Bai

Liu

et al. 2022

Veterinary Sciences

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The study was conducted to evaluate the effects of dietary chlorogenic acid supplementation on the growth performance, antioxidant function, and immune response of broiler breeders exposed to immune stress or high stocking density stress. The test was divided into two stress models. For the immune stress test, 198 birds were distributed into three experimental treatments with six replicates per treatment. The treatments were: (1) saline control (birds injected with saline and fed basal diet), (2) LPS group (birds injected with 0.5 mg LPS/kg body weight and fed basal diet), and (3) CGA + LPS group (birds injected with LPS and fed basal diet supplemented with 1 g/kg CGA. LPS was intraperitoneally injected from day 14, and then daily for 10 days. For the high stocking density stress model, 174 birds were distributed into three experimental treatments with six replicates per treatment. The treatments were: (1) controls (birds fed basal diet and raised at a stocking density of 14 broilers per m2), (2) high-density group (birds fed with basal diet and raised at a stocking density of 22 broilers per m2), and (3) high density + CGA group (birds fed with 1 g/kg CGA and raised at a stocking density of 22 broilers per m2). Results showed that LPS injection and high stocking density significantly decreased the body weight and feed intake of broiler breeders, while CGA supplementation increased feed intake of broiler breeders under LPS injection and high stocking density stress. Moreover, LPS injection and high stocking density increased the concentration of corticosterone in serum, and CGA addition remarkably downregulated serum corticosterone levels. The GSH level decreased with LPS injection and CGA increased the GSH concentration in the intestines of immune-stressed broiler breeders. LPS injection promoted the production of circulating proinflammatory cytokines (serum IL-1β and TNF-α) by 72 h after LPS injection. Dietary supplementation with CGA prevented the increase in serum TNF-α caused by LPS. These results suggest that dietary inclusion of 1 g/kg CGA could increase the feed intake of broiler breeders and alleviate the effects of inflammatory mediator stress and exposure to high stocking density.

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Section: Discussionmentioning

confidence: 99%

Effect of Dietary Chlorogenic Acid on Growth Performance, Antioxidant Function, and Immune Response of Broiler Breeders under Immune Stress and Stocking Density Stress

Bai

Liu

et al. 2022

Veterinary Sciences

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“…At present, CGA has been served as a feed supplement to improve the immunity and growth performance in livestock and poultry husbandry [18][19][20][21][22]. In aquaculture, the application of CGA is mostly focused on the growth, flesh quality, and lipid metabolism of the food fish [23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Chlorogenic Acid Supplementation in Koi (Cyprinus carpio) Diet: Growth Performance, Body Color, Antioxidant Activity, Serum Biochemical Parameters, and Immune Response

Wang

et al. 2022

Aquaculture Nutrition

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A ten-week feeding trial was performed to evaluate the effects of chlorogenic acid (CGA) on the growth, body color, antioxidant activity, serum biochemical parameters, and immune response of koi. Five diets were formulated to contain 0 (control), 200 (CGA-2), 400 (CGA-4), 600 (CGA-6), and 800 (CGA-8) mg/kg CGA for the rearing of experimental fish. By means of an analysis by polynomial orthogonal contrasts, it was determined that dietary supplementation with CGA had no significant effects on the growth performance of koi ( P > 0.05 ), while body color indices (redness, yellowness, and lightness) showed a linear order polynomial ( P < 0.05 ). A quadratic response was found for the relationship between total carotenoid in scale of koi and dietary CGA levels, with an optimum level of 381.84 mg/kg. Dietary CGA levels and lysozyme activity showed a cubic relationship polynomial model, with an optimum level of 383.89-975.73 mg/kg. TGF-β expression in the head-kidney of koi was obviously improved by 600 mg/kg CGA diet ( P < 0.05 ). And in regards to effect in serum, SOD and CAT activities was best explicated by a linear order polynomial, while the quadratic and cubic relationship polynomial models represented the GSH and GSH-Px activities, which the optimal supplementation level of CGA was estimated at 550.78 and 231.74-782.66 mg/kg, respectively. The relationship between ALT activity and CGA supplementation was best explicated by quadratic relationship polynomial, with an optimum level of 522.78 mg/kg. Based on the model observed, 522.78-600 mg/kg CGA was the recommended supplementation in diet that improved the body color, antioxidant activity, biochemical parameters, and immune response of koi.

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“…Based on our previous in vitro screening study on 22 kinds of immunopotentiators to determine the positive immune regulatory effects of immunopotentiators ( Gu, et al, 2020 ), chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG were chosen for further in vivo study. Shaoxing ducklings in 1-day-old were divided into 6 groups and subcutaneously injected with 0.5 mL saline, 0.4 mL chlorogenic acid (1 mg/mL), 0.7 mL β-D-Glucan (1 mg/mL), 0.4 mL astragalus flavone (5 mg/mL), 0.5 mL CpG-DNA (5 μg/mL), and 0.5 mL chicken IgG (1 mg/mL), respectively, once daily for 3 d. Fifteen days after the last immunopotentiator injection, the ducklings (5 ducklings per group) were injected with pentobarbital sodium (100 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, there are very few studies on the effects of immunopotentiators in ducks. Our previous studies demonstrated that chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG could enhance the immune effects of duck embryonic fibroblast cells ( DEFs ) in vitro ( Gu et al, 2020 ). In the present study, the effects of these 5 immunopotentiators were evaluated in vivo by determining spleen tissue antioxidant activity, apoptosis, proinflammatory cytokines, and immune-related gene expression.…”

Section: Introductionmentioning

confidence: 99%

Immunopotentiators improve the antioxidant defense, apoptosis, and immune response in Shaoxing ducklings

Liu

et al. 2022

Poultry Science

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The abuse of antibiotics for agricultural purposes has been under scrutiny. Therefore, there is an urgent need to find antibiotic substitutes in animal production. The effects of chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG on spleen antioxidant capacity, apoptosis, and the immune response in Shaoxing ducklings were investigated in this study. The ducklings treated with β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG showed significant reduction in catalase and superoxide dismutase activities. The five immunopotentiators facilitated caspase 3 expression and reduced Bcl2 expression in the spleen. Compared to the control group, the protein level of COX2 was significantly upregulated in the chlorogenic acid, CpG-DNA, and chicken IgG groups. The protein level of iNOS expression was significantly improved in all immunopotentiator groups, except for the astragalus flavone group. The five immunopotentiators induced IL-1β, IFN-α, IFN-β, TNF-α, RIG-I, TLR3 , and TLR7 gene expression. In summary, chlorogenic acid, β-D-Glucan, astragalus flavone, CpG-DNA, and chicken IgG, as immunopotentiators, improved the innate immune response in the ducklings, which not only provides a new avenue for the development of efficient approaches to prevent pathogen infections, but also offers an alternative to antibiotics in animal production.

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Effects of immunopotentiators on biochemical parameters, proinflammatory cytokine, and nonspecific immune responses in Shaoxing ducklings

Cited by 15 publications

References 32 publications

Effect of Dietary Chlorogenic Acid on Growth Performance, Antioxidant Function, and Immune Response of Broiler Breeders under Immune Stress and Stocking Density Stress

Effect of Dietary Chlorogenic Acid on Growth Performance, Antioxidant Function, and Immune Response of Broiler Breeders under Immune Stress and Stocking Density Stress

Evaluation of Chlorogenic Acid Supplementation in Koi (Cyprinus carpio) Diet: Growth Performance, Body Color, Antioxidant Activity, Serum Biochemical Parameters, and Immune Response

Immunopotentiators improve the antioxidant defense, apoptosis, and immune response in Shaoxing ducklings

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