Effects of in ovo injection of Manihot esculenta extract on hatchability and post-hatch performance of Sasso broiler chickens

Abstract

Manihot esculenta leaf meal in feed at post-hatch improves growth and egg production. One may question the efficacy of Manihot esculenta leaf extract for in ovo feeding. This study was conducted to determine the effects of in ovo injection of Manihot esculenta extract on hatchability, growth performance and biochemical parameters of Sasso broiler chickens. The hatching eggs from 38-week-old broiler breeders were set and at d 18 of incubation, 600 eggs with evidence of living embryos were assigned to 4 treatment groups of 150 eggs each. These groups were: (1) eggs without in ovo injection (negative control); (2) eggs perforated but no injection of any additive (positive control); (3) eggs perforated and injected with 0.1 ml of saline solution and (4) eggs perforated and injected with 0.1 ml of Manihot esculenta extract. Data were collected on hatching performance. During the post hatch growth phase, performance traits such as feed intake, weight gain, and feed conversion ratio were collected and at 84 days of age, blood samples were taken for determination of serum total proteins, triglycerides, aspartate aminotransferase (ASAT) and alanine amino transaminase (ALAT). The results showed that hatchability was similar in the negative control and Manihot esculenta extract groups but significantly higher (P < 0.05) than those of positive control and saline solution treatment groups. Chick quality was better in the groups with Manihot esculenta extract and saline solution application. Post-hatch, the body weight gain of birds from the Manihot esculenta extract group was higher than those in the groups with saline solution application and in the negative control and positive control groups.Manihot esculenta leaf extract increased significantly the triglyceride rate and reduced ALAT and ASAT concentration at hatch. It can be concluded that in ovo inoculation of 0.5 μg/ml Manihot esculenta leaf extract on d 18 of incubation into the eggs air chamber improved hatchability, chick quality and post-hatch growth of broilers.

In ovo effect of Soursop (Annona muricata L.) leaf extract on hatching and post-hatch performance of Noiler chickens

“…The result of this study agrees with Ngueda et al. (2021) , who reported that in ovo cassava leaf extract caused a significant increase in the overall quality scores of Sasso broiler chicks.…”

“…(2023) who injected clove and cinnamon extract in broiler chicken eggs. Both researchers reported increased chick weight while Ngueda et al. (2021) , who injected 0.5 µg of cassava leaf extract in broiler chicken eggs reported similarity in hatchability and mortality rate.…”

Potential Implication of in ovo Feeding of Phytogenics in Poultry Production

“…In contrast, N’nanle et al (2017) reported that in ovo administration of Moringa oleifera extract improved hatchability and resulted in heavier chickens at hatch. Similarly, Ngueda et al (2021) found that injection of Manihot esculenta extract solution on the 18th day of incubation improved hatchability and reduced total incubation duration. In other studies, Hajati et al (2014) reported that in ovo injection of grape seed extract ( GSE ) increased hatchability, while Heidary et al (2020) found that in ovo administration of nano curcumin decreased hatchability.…”

“… Ngueda et al (2021) studied the in ovo feeding of Manihot esculenta extract solution at day 18 of embryonic development of broilers and reported improved chick weight and chick quality. As comparison to the saline solution and the negative control groups, the authors reported that the weights of 1-d-old chicks in the Manihot esculenta extract group were higher.…”

“…It has been mostly reported that in ovo supplementation of phytogenic substances has been shown to influence chicken performance parameters and has a growth-promoting effect on poultry. Ngueda et al (2021) proposed that the early feeding of Manihot esculenta extract via an in ovo route on the 18th day of incubation is utilized by chick embryos along with yolk sac reserves and promotes the growth performance than those of the other group. Feed intake was higher in the Manihot esculenta group and FCR showed no significant differences across the treatments.…”