Effects of in vitro maturation on gene expression in rhesus monkey oocytes

Lee, Young S.; Latham, Keith E.; VandeVoort, Catherine A.

doi:10.1152/physiolgenomics.90281.2008

Cited by 43 publications

(28 citation statements)

References 115 publications

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“…Recent studies indicated alterations in the transzonal processes between the cumulus cells and oocyte with rhesus monkey IVM (20). We reported recently that the transcriptomes of IVM and VVM metaphase II stage oocytes displayed a very limited degree of difference, with only 59 mRNAs showing significant alterations, most of which were mRNAs that failed to decline in abundance appropriately (38). Here, we examine the transcriptomes of the companion cumulus cells.…”

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confidence: 96%

“…It is remarkable that the difference between IVM and VVM in the rhesus monkey, yielding such a profound effect on oocyte quality (38,(62)(63)(64)(65)(66), is just that the last 24 h of maturation in response to hormonal stimulation occurs within cumulus-oocyte complexes (COCs) that have been removed from the follicular environment. This indicates that follicular signals during the final 24 h of maturation must normally modulate cumulus cell and/or oocyte phenotype in a manner that confers high oocyte quality.…”

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confidence: 99%

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Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

Lee¹,

VandeVoort

Gaughan

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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Lee YS, VandeVoort CA, Gaughan JP, Midic U, Obradovic Z, Latham KE. Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome. Am J Physiol Endocrinol Metab 301: E196 -E209, 2011. First published April 12, 2011 doi:10.1152/ajpendo.00686.2010.-The elaboration of a quality oocyte is integrally linked to the correct developmental progression of cumulus cell phenotype. In humans and nonhuman primates, oocyte quality is diminished with in vitro maturation. To determine the changes in gene expression in rhesus monkey cumulus cells (CC) that occur during the final day prior to oocyte maturation and how these changes differ between in vitro (IVM) and in vivo maturation (VVM), we completed a detailed comparison of transcriptomes using the Affymetrix gene array. We observed a large number of genes differing in expression when comparing IVM-CC and VVM-CC directly but a much larger number of differences when comparing the transitions from the prematuration to the post-IVM and post-VVM states. We observed a truncation or delay in the normal pattern of gene regulation but also remarkable compensatory changes in gene expression during IVM. Among the genes affected by IVM are those that contribute to productive cell-cell interactions between cumulus cell and oocyte and between cumulus cells. Numerous genes involved in lipid metabolism are incorrectly regulated during IVM, and the synthesis of sex hormones appears not to be suppressed during IVM. We identified a panel of 24 marker genes, the expression of which should provide the foundation for understanding how IVM can be improved for monitoring IVM conditions and for diagnosing oocyte quality. oocyte quality; in vitro maturation LIFE BEGINS IN THE OOCYTE. The oocyte accumulates a remarkable and complex macromolecular reservoir that mediates and controls the initial development of each new individual while at the same time eliminating a range of molecules that promote gametogenesis but would otherwise impede early embryogenesis (8,36,37,67). Failure to complete these transitions appropriately yields an oocyte with limited developmental potential, unable to undergo fertilization or activation, or unable to sustain embryogenesis after fertilization and activation.Because of the key role of the oocyte in early development, understanding the molecular features that confer a high oocyte developmental potential is of paramount importance in reproductive biology. Such information will reveal the underlying mechanisms and processes that drive oogenesis and early embryogenesis. Moreover, such information will provide markers with which to screen and select high-quality oocytes for assisted reproduction in humans and could enhance success in agricultural reproductive biology and species conservation applications.The oocyte develops in close coordination with the somatic companion cells, particularly the cumulus oophorous cells, which maintain direct contact with the oocyte via transzonal processes and gap junctions until ovulation. Through its secretion...

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confidence: 96%

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confidence: 99%

Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

Lee¹,

VandeVoort

Gaughan

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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“…More recently, Lee et al, (2008) reported a small number of transcriptional differences between rhesus monkey oocytes matured in vitro and in vivo. Interestingly, approximately 90% of the identified transcripts were upregulated or overexpressed in IVM oocytes.…”

Section: Modulating Transcriptional Activity In the Oocyte Genomementioning

confidence: 99%

The antral follicle: a microenvironment for oocyte differentiation

Hennet

Combelles²

2012

Int. J. Dev. Biol.

152

119

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Mammalian reproduction hinges upon the timely ovulation of a fully differentiated oocyte. This event is the culmination of a complex and dynamic developmental relationship between the oocyte and the antral follicle housing it; the antral follicle constitutes a specialized microenvironment or niche, uniquely suited to the needs of the oocyte as it approaches ovulation. During this time, the oocyte must complete its final growth, capacitation, and nuclear and cytoplasmic maturation. Its microenvironment-the antral follicle-is in turn responsible for the integrity of these processes and the production of a high quality oocyte. Components of the antral follicle, including three distinct somatic cell types (theca, granulosa and cumulus), the basal lamina, and follicular fluid, each have active and regulatory roles in oocyte differentiation. Several milestones in antral folliculogenesis also have an influence on oocyte development. This review will discuss the antral follicle microenvironment with specific attention to its importance in oocyte differentiation. As assisted reproductive technologies (ART) often require stages of oocyte differentiation to occur in vitro rather than in vivo, current knowledge of the antral follicle microenvironment will also be discussed with respect to its clinical applications.

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“…24 Finally, overexpression of the MEST gene has been described following in vitro maturation of rhesus monkey oocytes. 25 Aberrant methylation at MEST is associated with certain forms of male infertility. Hypermethylation of MEST has been described in males with oligozoospermia, 26,27 and also idiopathic male infertility.…”

Section: Introductionmentioning

confidence: 99%

Variable imprinting of the MEST gene in human preimplantation embryos

et al. 2012

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There is evidence that expression and methylation of the imprinted paternally expressed gene 1/mesoderm-specific transcript homologue (PEG1/MEST) gene may be affected by assisted reproductive technologies (ARTs) and infertility. In this study, we sought to assess the imprinting status of the MEST gene in a large cohort of in vitro-derived human preimplantation embryos, in order to characterise potentially adverse effects of ART and infertility on this locus in early human development. Embryonic genomic DNA from morula or blastocyst stage embryos was screened for a transcribed AflIII polymorphism in MEST and imprinting analysis was then performed in cDNA libraries derived from these embryos. In 10 heterozygous embryos, MEST expression was monoallelic in seven embryos, predominantly monoallelic in two embryos, and biallelic in one embryo. Screening of cDNA derived from 61 additional human preimplantation embryos, for which DNA for genotyping was unavailable, identified eight embryos with expression originating from both alleles (biallelic or predominantly monoallelic). In some embryos, therefore, the onset of imprinted MEST expression occurs during late preimplantation development. Variability in MEST imprinting was observed in both in vitro fertilization and intracytoplasmic sperm injection-derived embryos. Biallelic or predominantly monoallelic MEST expression was not associated with any one cause of infertility. Characterisation of the main MEST isoforms revealed that isoform 2 was detected in early development and was itself variably imprinted between embryos. To our knowledge, this report constitutes the largest expression study to date of genomic imprinting in human preimplantation embryos and reveals that for some imprinted genes, contrasting imprinting states exist between embryos.

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Effects of in vitro maturation on gene expression in rhesus monkey oocytes

Cited by 43 publications

References 115 publications

Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

The antral follicle: a microenvironment for oocyte differentiation

Variable imprinting of the MEST gene in human preimplantation embryos

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