Effects of in vitro simulated gastrointestinal digestion on the antioxidant, α-glucosidase and α-amylase inhibitory activities of water-soluble polysaccharides from Opilia amentacea roxb fruit

Application of X‐ray diffraction and energy dispersive spectroscopy in the isolation of sulfated polysaccharide from Porphyra haitanensis and its antioxidant capacity under in vitro digestion

“…Digestion of Opilia amentacea roxb fruit polysaccharide markedly increased its ABTS+ radical scavenging capacity. 40 The DPPH radical scavenging capacity The DPPH radical scavenging capacity of P. haitanensis polysaccharide fraction (Fig. 7(D)) behaved very similarly to that of its ABTS+ radical scavenging capacity.…”

“…After gastric digestion and 1 h of intestinal digestion, however, their scavenging capacities were all markedly higher, in agreement with a previous report. Opilia amentacea roxb fruit polysaccharides had stronger superoxide radical scavenging capacity after in vitro digestion, 40 whereas lower Mw Laminaria japonica fucoidan (samples 1 and 2) has weaker superoxide radical scavenging activity. 41 This was not consistent with our result, which might be because the content of the hydroxyl groups in samples 1 and 2 was too low.…”

Multifarious Biological Applications and Toxic Hg2+ Sensing Potentiality of Biogenic Silver Nanoparticles Based on Securidaca inappendiculata Hassk Stem Extract

“…Inhibition of these enzymes (alpha-glucosidase and alpha-amylase) is one of the effective regimens in the treatment of diabetes. 69 , 70 The results from the evaluation of the in vitro antidiabetic effects of SISE AgNPs is shown in Figure 6 . As can be seen, SISE AgNPs displayed potent alpha-amylase and alpha-glucosidase inhibition activity at concentrations ranging from of 0.2–1.4 µg/mL.…”

Physicochemical properties, α‐amylase and α‐glucosidase inhibitory effects of the polysaccharide from leaves of Morus alba L. under simulated gastro‐intestinal digestion and its fermentation capability in vitro by human gut microbiota

“…The contents of uronic acid were determined using carbazole-sulphuric acid method and calculated by galacturonic acid standard curve (Li et al, 2018b). The contents of total carbohydrates were detected using phenol-sulphuric method with glucose as the standard (Kasipandi et al, 2019). The contents of proteins were measured by Bradford protein method, and bovine serum albumin was used as standard (Chan et al, 2009).…”

“…And the inhibitory effects of MLP-2 digestion fractions against the digestive enzymes were higher than those of polysaccharide fractions from mulberry fruit (Chen et al, 2016b). Polysaccharides could display α-amylase and α-glucosidase inhibitory action by physical interference and the direct binding with the digestive enzymes, which were closely dependent on their structure, conformation, monosaccharide proportion, chemical compositions and molecular weight distribution (Chen et al, 2016b, Nsor-Atindana, et al, 2012Li et al, 2018a;Chen et al, 2017;Yang et al, 2019;Kasipandi et al, 2019). In our study, the chemical compositions (sugar, uronic acid and protein contents), monosaccharide proportion and molecular weight of MLP-2 were all altered after the sequential simulated salivary, gastric and intestinal digestion, which might explain why MLP-2 did not inhibit the activities of α-amylase and α-glucosidase while its digested fractions displayed significantly enhanced inhibition against the digestive enzymes.…”