Effects of in vivo lidocaine administration at the time of ischemia and reperfusion on in vitro contractility of equine jejunal smooth muscle

Guschlbauer, Maria; Feige, Karsten; Geburek, Florian; Hoppe, Susanne; Hopster, Klaus; Pröpsting, Marcus J.; Huber, Korinna

doi:10.2460/ajvr.72.11.1449

Cited by 24 publications

(17 citation statements)

References 25 publications

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“…Following general anesthesia and ventral laparotomy in dorsal recumbency all horses underwent an IRIM as described previously (Guschlbauer et al 2010a(Guschlbauer et al , 2011. All animal procedures were approved by the State Office for Consumer Protection and Food Safety in accordance with the German Animal Welfare Law.…”

Section: Surgical Procedures Of Ischemia-reperfusion Injurymentioning

confidence: 99%

“…This dosage was set to be optimal effective by preliminary in vitro studies which showed a dose-dependent response of in vitro measured contractility to lidocaine reaching "steady-state" at lidocaine concentrations ranging between 20 and 100 mg/L (Guschlbauer et al 2010a). Tissue was prepared as previously described by (Guschlbauer et al 2010a(Guschlbauer et al , 2011. Briefly, strips of the circular smooth muscle of equal size and weight were prepared from each horse of IRL1 and IRL2 and mounted into the force measurement apparatus fitted with isometric force transducers.…”

Section: Tissue Preparationmentioning

confidence: 99%

“…during the ischemic period of an IRIM. Lidocaine tissue concentrations in jejunal smooth muscle achieved 133.9±24.5 ng/mg tissue wet weight measured directly after resection (Guschlbauer et al 2011). Lack of lidocaine pharmacokinetics in healthy and IRIM horses causes difficulties in extrapolation of in vitro effective lidocaine concentrations of organ bath buffers into in vivo effective CRI concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…Experiments were performed in healthy and artificially IR challenged jejunal smooth muscle, demonstrating that intestinal smooth muscle contractility responded dose-dependently to lidocaine supplementation in vitro and that IR-injured smooth muscle is more susceptible to lidocaine (Guschlbauer et al 2010a(Guschlbauer et al , 2011. Lidocaine was able to restore contractility of IR-injured smooth muscle to the level of healthy smooth muscle, indicating a repair mechanism (Guschlbauer et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

“…Hence lidocaine is also able to prevent smooth muscle injury provoked by artificial IR. When additional in vitro lidocaine supplementation was performed after in vivo application, contractility could further be improved (Guschlbauer et al 2011). However, very high dosages of lidocaine in vitro reduce contractile performance of smooth muscle (decreasing in vitro contractility after supplementing 100-400 mg/l) indicating an accumulation within the muscle cell (Guschlbauer et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

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In vitro analysed effects of two different in vivo administered lidocaine dosageson the equine jejunal smooth muscle challenged by an ischemia-reperfusion-injury-model (IRIM)

Uhlendorf¹,

Guschlbauer²,

Hoppe³

et al. 2012

PHK

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SummaryLidocaine as a prokinetic drug is acting dose-dependently on the ischemia-reperfusion (IR) injured equine jejunal smooth muscle. The aim of the study was to examine the effects of different dosages of lidocaine on jejunal smooth muscle contractility in vitro as a basic research approach to examine the potential mechanism of lidocaine. Sustainability of this in vivo effect of applied lidocaine was tested under in vitro conditions. Hypothesis: Application of a higher initial dose of lidocaine during IR in vivo could change, improve or even impair the contractility effects on jejunal smooth muscle in vitro. 12 horses received either a 1.3 mg/kg (IRL1; N = 7) or 2.6 mg/kg (IRL2; N = 5) lidocaine bolus infusion over 10 minutes followed by 0.05 mg/kg/min intravenously for 5 minutes while artificial IR injury on the jejunum was induced. To examine the effects of lidocaine on jejunal smooth muscle function, isometric force performance (amplitude, frequency and contractility) was measured in vitro at two different times (t1,t2). The influence of either in vitro lidocaine supplementation (KHB+L) or no supplementation (KHB) was studied to assess the sustainability of lidocaine effects. IRL2 KHB+L showed a significant higher frequency of contraction at t2 compared to IRL1KHB+L. Amplitude of contractions and contractility were significantly decreased in the IRL1 KHB tissues compared to IRL1 KHB+L at t2. The IRL2 KHB tissues at t2 showed a significant decrease of frequency and amplitude of contractions compared to the IRL2 KHB+L. There was a significant decrease in frequency and an increase in amplitude of contractions and in contractility in IRL 1 KHB+L from t1 to t2. In IRL1 KHB a significant decrease in frequency was observed but increase in amplitude of contractions and contractility was lacking from t1 to t2. IRL2 KHB expressed a significant decrease in frequency and a significant increase in amplitude from t1 to t2, while amplitude, frequency and contractility in IRL2 KHB+L were not influenced by time. High dosage lidocaine bolus infusion with further supplementation increased frequency of contractions and maintained amplitude of contractions over the experimental time. This in vitro study suggested that immediate application of a high bolus dosage may improve protective effects of lidocaine furthermore in vitro. At the moment, no pharmacokinetic data for a 2.6 mg/kg over 10 min. lidocaine bolus in vivo is available. Recommending a higher initial lidocaine bolus in vivo, especially because of negative side effects, requires further investigation.Keywords: lidocaine / equine jejunum / contractility / smooth muscle / prokinetic effects / anesthesiology / horse In vitro untersuchte Effekte von zwei unterschiedlichen in vivo verabreichten Lidokaindosierungen auf die glatte Muskulatur des equinen Jejunums in einem Ischämie-Reperfusions-Modell Lidokain wirkt dosisabhängig prokinetisch auf die Ischämie-Reperfusion (IR) geschädigte glatte Muskulatur des equinen Jejunums. Ziel dieser Studie war, die Effekte unterschiedlic...

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Section: Surgical Procedures Of Ischemia-reperfusion Injurymentioning

confidence: 99%