Effects of inbreeding on growth and survival rates, and immune responses of ridgetail white prawn Exopalaemon carinicauda against the infection of Vibrio parahaemolyticus

Wang, Jiajia; Ge, Qianqian; Li, Jitao

doi:10.1016/j.aquaculture.2019.734755

Cited by 9 publications

(4 citation statements)

References 59 publications

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“…The full-sibling family establishment process for EC4 inbred line is depicted in Figure 1 and by repeating the process, 11 generations of full-sibling family were obtained simultaneously until the 2017. As our previous study has shown, the F 1 generation full-sibling family was established by brother×sister mating, and the F 2 generation full-sibling family was obtained from the F 1 generation with brother×sister mating, and the F 3 , F 4 , F 5 , F 6 , F 7 , F 8 , F 9 , F 10 , and F 11 generations were obtained in a similar way [28]. Five groups (F 7 , F 8 , F 9 , F 10 , and F 11 ) with different levels of inbreeding coefficient (F = 0:785, F = 0:816, F = 0:859, F = 0:886, and F = 0:908) were analyzed in this study.…”

Section: Establishment Of Inbred Lines and Controlmentioning

confidence: 99%

Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda

Wang

et al. 2020

International Journal of Genomics

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The Exopalaemon carinicauda could be a useful crustacean laboratory animal in many research fields. We newly established an inbred line of Exopalaemon carinicauda named EC4 inbred line by brother×sister mating and keeping to F11 generation. Trends in heterozygosity in the process of producing EC4 inbred line were examined through the characterization of polymorphisms based on gene frequencies of SNP and EST-SSR loci. The results demonstrated that the number of alleles (N), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) gradually decreased with the increase of inbreeding generations. The genetic detection results indicated that 9 (29.03%, 9/31) of the SNP loci and 15 (32.61%, 15/46) of the EST-SSR loci were homozygous in F11 generation of EC4 inbred line. The variation of the growth-related traits, the immune responses, and antioxidant status were described in experimental full-sibling inbred populations of E. carinicauda at five levels of inbreeding coefficient (F=0.785, F=0.816, F=0.859, F=0.886, F=0.908) under controlled laboratory conditions. The body weight, body length, and survival rate in EC4 inbred line of all generations were less than the control population. Inbreeding affected the antibacterial activity, phenoloxidase (PO) activity, and superoxide dismutase (SOD) which decreased at the eleventh generation of EC4 inbred line. This study demonstrated that inbreeding had a negative effect on the economic traits and immune response, but our inbred line was established successfully until F11 and confirmed by genetic detection using SNP and EST-SSR loci.

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Section: Establishment Of Inbred Lines and Controlmentioning

confidence: 99%

Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda

Wang

et al. 2020

International Journal of Genomics

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“…However, aquaculture production and large-scale cultivation are still restricted by the supply of high-quality broodstock. In addition to its economic value, E. carinicauda is a useful experimental organism in the field of crustacean developmental biology due to its transparent body, large egg, and high reproductive capacity, and short reproductive cycle of only 2-3 months (Yuan et al, 2017;Liang et al, 2020;Wang et al, 2020). The female shrimps generate at least three consecutive ovarian development during reproductive stage, but the ovaries of some shrimps gradually atrophy into cystic tubes after first ovarian mature.…”

Section: Introductionmentioning

confidence: 99%

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

Wang

Li²,

Ge³

et al. 2022

Front. Mar. Sci.

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The ridgetail white shrimp Exopalaemon carinicauda has the potential to be used as a useful experimental organism in the field of crustacean research due to its transparent body, large egg, and short reproductive cycle. However, little is known about the regulatory mechanisms of ovarian maturation in E. carinicauda. In this study, we applied PacBio single-molecule long-read sequencing technology to unveil the whole transcriptome landscape of Exopalaemon carinicauda to better understand the molecular mechanisms of ovarian development. A total of 49.05 G polymerase read bases were generated, finally, 15, 151 unigenes were successfully identified with a mean length of 4, 257 bp and an N50 of 4, 884 bp from ovary tissues (five different ovarian developmental stages). A total of 14, 007 unigenes were successfully annotated in at least one public database. In addition, 8, 861 coding sequences, 4, 594 lncRNAs and 895 transcription factors were identified. Furthermore, we performed RNA-Seq analysis combined with Iso-Seq results to investigate ovarian maturation regulation mechanism and validated the results by quantitative real-time PCR. We annotated five vitellogenin isoforms in the full-length transcriptome, meanwhile these five genes and vitellogenin receptor suggested an important role during previtellogenesis of E. carinicauda. Genes were involved in PI3K-Akt signaling pathway, retinol metabolism, cell cycle and hedgehog signaling pathway, which showed obvious associations with ovarian maturation of E. carinicauda. We identified 40 DEGs, such as forkhead box protein L2, which may be related to ovarian development in the comparisons. The expression profiles of genes such as ecdysone receptor, ecdysone-induced protein 74EF, ecdysone inducible gene E75 and post-molt protein suggest that they were significantly associated with reproductive molting in the ovarian mature stage. In conclusion, the present study identified important genes and pathways involved in ovarian maturation, which might be useful for studying the reproductive regulation and mechanisms of ovarian maturation in E. carinicauda.

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“…The genetic degradation of breeding stocks leads to inbreeding depression, mainly manifested by poor growth performance, and which has been observed in Drosophila melanogaster (Latter et al, 1995), Penaeus stylirostris (Bierne et al, 2000), Litopenaeus vannamei (Moss et al, 2007), M . nipponense (Fu & Jin, 2018) and Exopalaemon carinicauda (Wang et al, 2020). Hybridization is one of the means to improve the genetic diversity of populations and eliminate inbreeding depression, by inducing heterosis in the hybrid offspring.…”

Section: Introductionmentioning

confidence: 99%

De novo transcriptome analysis reveals possible heterosis for growth, immunity and anti‐oxidation of Macrobrachium nipponense hybrid offspring and parent populations

Fan

et al. 2021

Aquaculture Research

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The oriental river prawn Macrobrachium nipponense is widely distributed in the estuaries of Asian countries, such as China, and is specifically adapted to thrive in brackish water environments (Li et al., 2019). Due to its rapid growth rate, short reproduction cycle and high tolerance to various environmental conditions (Li et al., 2019), the farming output of M. nipponense has exceeded 237.1 kilotons in China (FAO, 2020), representing one of the most important sources of protein available on the market. However, it is precisely because of such large-scale breeding that a series of problems have occurred in M. nipponense farms, such as genetic retrogression of breeding stocks and the spread of poor farming techniques (Fu & Jin, 2018). These challenges have caused a setback in the M. nipponense aquaculture industry in China. Due to multiple-generation reproduction in a small population, inbreeding existed in Macrobrachium nipponense, which caused germplasm degradation, poor immune tolerance and even precocious puberty

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Effects of inbreeding on growth and survival rates, and immune responses of ridgetail white prawn Exopalaemon carinicauda against the infection of Vibrio parahaemolyticus

Cited by 9 publications

References 59 publications

Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda

Effects of Inbreeding on Genetic Characteristic, Growth, Survival Rates, and Immune Responses of a New Inbred Line of Exopalaemon carinicauda

Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analysis Provide Insights Into the Ovarian Maturation of Exopalaemon carinicauda

De novo transcriptome analysis reveals possible heterosis for growth, immunity and anti‐oxidation of Macrobrachium nipponense hybrid offspring and parent populations

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