In a laser tracking control system, a motor drives the load to achieve continuous forward and reverse rotation speed control, and the magnitude of the load changes periodically during the motor rotation to realize tracking. Based on these characteristics, we propose an integral-separated improved variable universe fuzzy Proportional Integral(PI) control method with torque feedforward compensation method in this study. The proposed method achieves the control performances of fast response, high steady-state accuracy, and strong anti-interference. The conventional fuzzy PI control method achieves good performance only in the unidirectional acceleration of a motor; therefore, we have improved its fuzzy rules, and designed a novel universe contraction-expansion factor. Additionally, a load torque observer is introduced to observe and compensate for the external load and to further improve the anti-interference performance of the tracking control system. The experimental results demonstrate that the integral-separated improved variable-universe fuzzy PI control method achieves better performance in the forward and reverse speed regulation processes of the motor. The overshoot is 92% less than that of the conventional PI control when the target speed is set to ± 500 rpm. The Luenberger observer is introduced to observe and compensate for the load torque. After the stabilizing the speed and applying an external load of 0.2 N·m to the motor, the maximum speed reduction is observed to be 59% less than that obtained using the conventional PI control. The improved PI control method satisfies the speed loop control requirements of the laser tracking control system and can improve the steady-state tracking accuracy and robustness of the system.