Effects of incorporation of granule-lyophilised platelet-rich fibrin into polyvinyl alcohol hydrogel on wound healing

Abstract

Dressings are commonly used to treat skin wounds. In this study, we aimed to develop a new scaffold composed of a polyvinyl alcohol (PVA) hydrogel containing granule-lyophilised platelet-rich fibrin (G-L-PRF) as a dressing. G-L-PRF was prepared by freeze-drying and was then incorporated into PVA hydrogel by freezing-thawing. Notably, the mechanical strength and degradation rate of the scaffold were found to be related to G-L-PRF concentrations, reaching 6.451 × 10−2 MPa and 17–22%, respectively, at a concentration of 1%. However, the strength decreased and the degradation was accelerated when the G-L-PRF concentration was over 1%. The elastic properties and biocompatibility of the scaffold were independent of G-L-PRF concentration, and both showed excellent elasticity and biocompatibility. The release of vascular endothelial growth factor and platelet-derived growth factor-AB was no significant time dependent. Additionally, application of 1% G-L-PRF/PVA to acute full-thickness dorsal skin wounds accelerated wound closure at days 7 and 9. Healing also increased on day 11. Histological and immunohistochemical analyses showed that the scaffold enhanced granulation tissue, maturity, collagen deposition, and new vessel formation. These results demonstrated that the prepared G-L-PRF/PVA scaffolds accelerated wound healing in acute full-thickness skin wounds, suggesting potential applications as an ideal wound dressing.

Polymeric Hydrogel Scaffolds: Skin Tissue Engineering and Regeneration

“…In the past, topical wounds are typically treated and managed with traditional dressings. 35 Xu et al fabricated a novel granule-lyophilised platelet-rich fibrin-loaded polyvinyl alcohol hydrogel scaffolds as an ideal wound dressing material for skin tissue regeneration. Notably, the characterisation studies revealed the excellent morphology, biocompatibility, biodegradability (17-22%), and mechanical strength (6.451×10−2MPa) of this granulelyophilised platelet-rich fibrin loaded polyvinyl alcohol hydrogel scaffolds.…”

Potential of Lyophilized Platelet Concentrates for Craniofacial Tissue Regenerative Therapies

“…Interestingly, in contrast to the nontreated group, this study revealed a significant (p < 0.01) fivefold increase in blood vessel density in the LPC group. Recently, Xu et al [15] suggested that the vascularization process was accelerated in their skin wound study in mice after using a scaffold composed of polyvinyl alcohol (PVA) hydrogel and LPCs as a dressing within 9 days post-surgery. Hence, they believe the neo-angiogenesis could provide oxygen and nutrient to the surgical site, thus promoting protein synthesis.…”

“…Finally, due to the requirement for the need for their use within a short time-frame, as they cannot be easily stored or transported, their application is not well suited for emergency care medicine [14]. These issues restrict the use of PCs for broader therapeutic applications [15].…”

The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts

“…PRP has been incorporated into collagen gel [ 15 ] and into the scaffold composed of PRP and hyaluronic acid [ 19 ], via the incorporation of granules of lyophilized platelet-rich fibrin into a poly(vinyl alcohol) (PVA) hydrogel [ 23 ], via the binding of platelet-rich plasma [ 24 ] and the growth factors of interest [ 25 ] onto chemically modified or activated polycaprolactone (PCL) nanofibers, or via platelet lysate incorporation into the fibrin layer created on the surface of an electrospun nanofibrous blend of poly(L-lactide- co -ε-caprolactone) and poly(ε-caprolactone) [ 26 ].…”