Effects of increased salinity on glucose and maltose composition of<i>Biomphalaria glabrata</i>snails infected with<i>Schistosoma mansoni</i>as determined by high-performance thin-layer chromatography-densitometry

Popovic, Nevena; Fried, Bernard; Sherma, Joseph

doi:10.1556/jpc.26.2013.2.6

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…Similar increases were seen in the results of the Popovic et al study [2]; it was suggested that higher metabolic rates could be present in the older snails after two weeks, resulting in fluctuations in the sugar content.…”

Section: Discussionsupporting

confidence: 85%

“…Therefore, it is important to OPEN ACCESS focus on these sugars when conducting carbohydrate analysis. Some HPTLC studies have been done on influences of the carbohydrate composition of B. glabrata, including salinity [2]. In the salinity study, it was found that increased salinity did not affect the carbohydrate composition of the snail's digestive gonad gland complex (DGG).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry

et al. 2015

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Abstract:The effects of 5, 25, and 40 Echinostoma caproni miracidia on the sugar content of young adult and mature adult Biomphalaria glabrata were studied using high performance thin layer chromatography (HPTLC)-densitometry. Analysis was done on the snail's digestive gland gonad complex (DGG) at two and four weeks postmiracidial exposure. The sugars were extracted from the DGG using 70% ethanol and analyzed on silica gel HPTLC plates with a preadsorbent zone using 1-butanol-glacial acetic acid-diethyl ether-deionized water (27:18:5:3) mobile phase. The separated bands were then detected using alpha-naphthol-sulfuric reagent and quantified by densitometry at 515 nm. Significant differences were found in the maltose content between two and four weeks post exposure for both age groups. Additionally, significantly lower maltose and glucose levels were observed in the high exposure groups of both ages.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry

et al. 2015

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Ethylamine as new derivatization reagent differentiating reducing from non-reducing saccharides

Schwack,

Heilmann,

Morlock

2023

JPC-J Planar Chromat

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Typical derivatization reagents for saccharides in high-performance thin-layer chromatography, like 2-naphthol sulfuric acid, aniline diphenylamine orthophosphoric acid, or p-aminobenzoic acid, generally detect both reducing and non-reducing saccharides. A new reagent was found with ethylamine, specifically reacting with reducing saccharides on normal-phase silica gel plates, resulting in strongly fluorescent zones after heating the plate at 150 °C for 15 min. In contrast, non-reducing saccharides generally did not reveal fluorescent signals tested with 26 different saccharides. Optimal chromatographic separation was achieved with a mixture of 2-propyl acetate, methanol, and water with 1 mg/mL natural product reagent A when the plate was twofold developed. The high sensitivity of the ethylamine derivatization was shown with mean limits of detection and quantification of 10 and 30 ng per zone, respectively, calculated by different methods for selected mono- and disaccharides. The developed method has exemplarily been used for the digestion control of starch by α-amylase, the determination of lactose in lactose-free milk, and for the quantitative and qualitative study of honey. The analysis of honey gave an excellent example of the advantageous consecutive derivatization with ethylamine and aniline diphenylamine orthophosphoric acid reagent as reagent sequence to detect the coelution of reducing and non-reducing saccharides.

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Application of thin-layer chromatography to the analysis of saccharides

Poole

2023

Instrumental Thin-Layer Chromatography

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Effects of increased salinity on glucose and maltose composition ofBiomphalaria glabratasnails infected withSchistosoma mansonias determined by high-performance thin-layer chromatography-densitometry

Cited by 5 publications

References 11 publications

Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry

Effects of Different Levels of Echinostoma caproni Miracidial Dose on Glucose and Maltose Composition of Biomphalaria glabrata Snails as Determined by High Performance Thin-Layer Chromatography-Densitometry

Ethylamine as new derivatization reagent differentiating reducing from non-reducing saccharides

Application of thin-layer chromatography to the analysis of saccharides

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