Effects of indomethacin on estradiol-induced attenuation of hypoxic vasoconstriction in lamb lungs

Gordon, John B.; Wetzel, Randall C.; McGeady, M L; Adkinson, N. F.; Sylvester, J. T.

doi:10.1152/jappl.1986.61.6.2116

Cited by 18 publications

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“…(Pediatr Res 23: 580-584, 1988) acin-enhanced normoxic PVR in perfused left lower lobes of both premature and mature newborns; however, in this species Abbreviations HPV was potentiated in both age groups (25). In isolated perfused lungs from 2-to 3-month-old lambs, however, there was no effect 6-keto-PGF,,, 6-keto-prostaglandin F,, of indomethacin on steady-state pulmonary pressures during PVR, pulmonary vascular resistance graded hypoxia (26,27). HPV, hypoxic pulmonary vasoconstriction…”

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confidence: 81%

“…This amount of indomethacin has the American Heart Association with funds contributed in part from the Heart ~r e v i o u~l~ been shown to inhibit cyclooxygena~e activity (26, Association of Central Maryland. 27). …”

Section: Methodsmentioning

confidence: 99%

“…This preparation was only slightly modified from that used previously for the study of older animals (26)(27)(28). Briefly, lambs were anesthetized with ketamine (50 mg/kg, intramuscular) and supplemental oxygen was provided by a loose-fitting mask.…”

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confidence: 99%

“…After centrifugation, the supernatant was stored at -16" C until radioimmunoassay for 6-keto-PGF,, was performed as previously described (26,27).…”

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Age-Dependent Effects of Indomethacin on Hypoxic Vasoconstriction in Neonatal Lamb Lungs

Tod

et al. 1988

Pediatr Res

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ABSTRACT. Although smooth muscle is abundant in the PVR decreases dramatically when ventilation is initiated at pulmonary vessels of young animals at birth, it is not clear birth (1)(2)(3)(4)(5)(6)(7). This is likely a consequence of mechanical distention if these vessels respond more vigorously to hypoxia than of the lung (1, 2), increased oxygen tension (1, 2), and release of the less muscular vessels of older neonates. To determine dilator PG (2-6). Subsequent decreases in normoxic PVR have the effect of age on the pulmonary vascular response to been well documented in the growing piglet (7) and in other hypoxia during the neonatal period in a single species, we species (2,8,9). In contrast, age-related changes in HPV are less measured the steady-state stimulus-response relationship clear. It has been suggested that the extent of arteriolar muscubetween inspired oxygen tension (200, 50, 30 and 0 mm larization determines the vigor of HPV (10-12). In this case, the Hg) and pulmonary artery pressure-flow curves in isolated newborn animal with its highly muscularized vessels (2, 7, 9, 10) blood perfused lungs from 2-to 4-and 12-to 14-day-old should have a more vigorous hypoxic response than the older lambs. Hypoxic vasoconstriction was attenuated in the animal. Although some studies show an age-dependent decrease younger newborns at an inspired oxygen tension of 50 mm in HPV (8, 13), others report an increase with age (10, 14, 15). Hg, but not at the other oxygen tensions. To determine ifOne possible explanation for these apparently conflicting rethis age-related difference was due to differences in mod-sults is that the vigor of HPV may be influenced by factors other ulation of hypoxic vasoconstriction by cyclooxygenase than vascular muscularization. For instance, there could be ageproducts, we assayed the metabolite of prostacyclin, 6-dependent differences in the modulation of HPV by pulmonary keto-prostaglandin El, in the perfusate and determined the vasodilators. Although vasodilators such as PGI, are released effects of indomethacin (40 pglml) on the hypoxic stimulus-during HPV in both young neonates and mature animals (1 6, response relationship. There was no age-related difference 17), the action and metabolism of various PG may vary with age in perfusate concentration of 6-keto-prostaglandin El, at (18, 19). For example, the vasodilator PGE2 (20) is more slowly any oxygen tension. However, indomethacin reversed the metabolized in the neonate (18). PGD2 is a pulmonary vasodiage-dependent attenuation of hypoxic vasoconstriction at lator in neonatal lambs, but a vasoconstrictor in older animals inspired oxygen tension = 50 mm Hg such that in indo-(21-23). Such differences may be partially responsible for the methacin-treated lungs pulmonary vasomotor tone was variable effects of cyclooxygenase inhibition on the developing higher in 2-to 4-day-old lungs than in 12-to 14-day-old pulmonary vasculature. In the intact pig, meclofenamate inlungs. This marked enhancement of hypoxic reactivity by creased normoxic PV...

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mentioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

“…After centrifugation, the supernatant was stored at -16" C until radioimmunoassay for 6-keto-PGF,, was performed as previously described (26,27).…”

mentioning

confidence: 99%

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Age-Dependent Effects of Indomethacin on Hypoxic Vasoconstriction in Neonatal Lamb Lungs

Tod

et al. 1988

Pediatr Res

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“…The effects of endogenous sex hormones on HPV were studied by Wetzel et al [23,24] and Gordon et al [25] in isolated sheep lungs. However, these studies did not examine the effects of estrogen depletion in adult animals.…”

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confidence: 99%

The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings

Patel

Lahm

Crisostomo

et al. 2008

Journal of Surgical Research

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Estrogen-induced microvilli and microvillar channels and entrapment of surfactant-lipids by alveolar type I cells of bovine lung

Atwal

1999

Anat. Rec.

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The ATI cells are simple, flat squamous epithelial cells, which are evolved to function as a component of the alveolar-capillary membrane, ideally designed for gaseous exchange. They inherently lack an active metabolic machinery and lead a precarious existence in the face of hostile environment. On the other hand, the ATI cells of the lung of ruminating animals are endowed with structure-functional properties which enable them to exert a selective barrier function against a wide range of osmotic pressure gradients at their luminal surface. Such gradients are created by a complex gaseous homeostasis due to expectoration of several gases and volatile fatty acids originating from the complex stomach of the ruminants.The purpose of this study is to examine the effect of estradiol propionate on the ultrastructure of the ATI cells and their interaction with the surfactant lipids. The lungs of estrogen and dexamethasone treated male calves were harvested for electromicroscopic examination. The evidence is presented that estradiol induced the formation of microvilli and microvillar channels at the luminal surface. At these regional modifications, intense interactions with the surfactant lipids and their entrapment into the pathways of endocytosis, took place in the squamous part of the ATI cells.Concurrently, large basal protrusions ended up as long lamellipods deep into the alveolar interstitium. The filamentous cytoskeletal network and microtubules intermixed with the translocated organelles such as Golgi apparatus and associated coated and uncoated vesicles.The results of this study support the hypothesis that estrogen regulate the selective barrier-function of the ATI cells. The entrapment of surfactant lipids under the influence of estrogen by ATI cells is a significant change perhaps in response to extracellular stimuli and expression of transmembrane receptors. It implies that these epithelial cells are specially evolved to adapt to a complex gaseous homeostasis in the lung of the ruminating ungulates. Anat Rec 256:300-320, 1999. (Weibel, 1985). The long cytoplasmic flaps extend from the perikaryon of the cells and being uniformly isodiametric/isometric in their planar outline at their squamous part, correlate well with

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Effects of indomethacin on estradiol-induced attenuation of hypoxic vasoconstriction in lamb lungs

Cited by 18 publications

References 0 publications

Age-Dependent Effects of Indomethacin on Hypoxic Vasoconstriction in Neonatal Lamb Lungs

Age-Dependent Effects of Indomethacin on Hypoxic Vasoconstriction in Neonatal Lamb Lungs

The Effects of Endogenous Sex Hormones and Acute Hypoxia on Vasoconstriction in Isolated Rat Pulmonary Artery Rings

Estrogen-induced microvilli and microvillar channels and entrapment of surfactant-lipids by alveolar type I cells of bovine lung

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