Effects of injecting chitin particles on the innate immune response of gilthead seabream (L.)

Esteban, María Ángeles; Mulero, Víctoriano; Ortuño, Jesús; Meseguer, José

doi:10.1006/fsim.2000.0271

Cited by 85 publications

(51 citation statements)

References 31 publications

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“…This was greater in the groups of supplemented fish, and a favorable effect was observed in relation to inflammation and resistance, which corroborates results of others (Esteban et al 2000, Ortuño et al 2002, Reque et al 2010.…”

Section: Discussionsupporting

confidence: 91%

Inflammatory responses of Nile tilapia Oreochromis niloticus to Streptococcus agalactiae: effects of vaccination and yeast diet supplement

Salvador¹,

Toazza²,

Moraes³

et al. 2012

Dis. Aquat. Org.

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This study evaluated the effects of dietary supplementation with 0.3% Saccharomyces cerevisiae yeast cell wall and of vaccination against Streptococcus agalactiae on the cellular component of acute inflammation induced in the coelomic cavity of Nile tilapia Oreochromis niloticus and on survival of the fish after challenge. A total of 84 tilapia of mean (± SD) weight 125.0 ± 1.5 g were distributed among twelve 310 l fiberglass tanks according to a 2 × 2 × 3 factorial design in the following manner: with and without supplementation; 2 stimulations (oily solution without S. agalactiae vaccine and vaccination); 15 d later all fish were intracoelomically challenged with 10 8 CFU ml -1 of a homologous strain of S. agalactiae, and evaluated after 6, 24 and 48 h, with 7 replicates. The fish received the non-supplemented or supplemented diet for a total of 77 d. The vaccination was performed on the 60th day, intracoelomically, as a single injection of 0.5 ml of the vaccine containing 10 8 CFU ml −1. Fifteen days later, all the fish were challenged with S. agalactiae by means of an intracoelomic inoculation of 10 8 CFU ml −1. No mortality was observed among the supplemented fish. The fish that were fed the non-supplemented diet and immunized with the bacterium presented a mortality rate of 28.5%. Among the non-supplemented and nonimmunized fish, the mortality rate was 38.09%. Supplementation, in both vaccinated and nonvaccinated fish, induced larger accumulations of thrombocytes, lymphocytes and macrophages at the inflammatory focus. The results suggest that supplementation with 0.3% yeast cell wall, in both vaccinated and non-vaccinated fish, improved the in flammatory response of the fish and protected against the challenge. Vaccination increased the defense response, but the effect was stronger when associated with supplementation with S. cerevisiae. KEY WORDS: Inflammation · Saccharomyces cerevisiae · Vaccination · Streptococcus agalactiae · ChallengeResale or republication not permitted without written consent of the publisher

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Section: Discussionsupporting

confidence: 91%

Inflammatory responses of Nile tilapia Oreochromis niloticus to Streptococcus agalactiae: effects of vaccination and yeast diet supplement

Salvador¹,

Toazza²,

Moraes³

et al. 2012

Dis. Aquat. Org.

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show abstract

“…In the above study, the vitamin A supplements were up to five times the normal vitamin requirement, which are not comparable to the supplements assayed in this work. For other substances, such as chitin, injection was also more rapid and e#ective than oral administration [37,44].…”

mentioning

confidence: 98%

Changes in some innate defence parameters of seabream (Sparus aurata L.) induced by retinol acetate

Cuesta

Ortuño

Rodrı́guez

et al. 2002

Fish & Shellfish Immunology

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“…This has allowed us to get a lot of information about the regulation of the seabream immune response, and concretely the NCC activity. Thus, we have shown in vitro and/or in vivo modulation of seabream NCC activity by vitamins C (Cuesta et al, 2002b), E , A (Cuesta et al, 2003b) and D3 (Cerezuela et al, 2009), chitin (Cuesta et al, 2003c;Esteban et al, 2000Esteban et al, , 2001, levamisole (Cuesta et al, 2002c), lactoferrin , melatonin (Cuesta et al, 2008a), propolis (Cuesta et al, 2005b), inulin (Cerezuela et al, 2008), unmethylated oligodeoxynucleotides (ODNs) containing cytosine-phosphodiester-guanosine (CpG) motifs (Cuesta et al, 2008b(Cuesta et al, , 2008c, probiotic bacteria Salinas et al, 2005Salinas et al, , 2006Salinas et al, , 2008, yeast (Cuesta et al, 2007;Ortuño et al, 2002;Reyes-Becerril et al, 2008;Rodríguez et al, 2003), fungi (Rodríguez et al, 2004), virus , environmental contaminants (p,p´-DDE and lindane) (Cuesta et al, 2008d) or stress factors (air exposure, crowding and anaesthetics) (Cuesta et al, 2003d). In general, we have demonstrated great NCC increments after these treatments.…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 83%

Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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Effects of injecting chitin particles on the innate immune response of gilthead seabream (L.)

Cited by 85 publications

References 31 publications

Inflammatory responses of Nile tilapia Oreochromis niloticus to Streptococcus agalactiae: effects of vaccination and yeast diet supplement

Inflammatory responses of Nile tilapia Oreochromis niloticus to Streptococcus agalactiae: effects of vaccination and yeast diet supplement

Changes in some innate defence parameters of seabream (Sparus aurata L.) induced by retinol acetate

Fighting Virus and Parasites with Fish Cytotoxic Cells

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