The worldwide search for eco-friendly energy sources has raised interest in biodiesel as a possible replacement for traditional fossil fuels. The goal of this study is to thoroughly examine the benefits and drawbacks of biodiesel while considering possible future developments for engine fuels. Researchers have focused a lot of interest on biodiesel since its introduction as a renewable alternative fuel. The fundamental components of biodiesel are created through the transesterification of plant, animal, or even waste cooking oil. Biodiesel is used to fully use natural resources and lessen the severity of oil shortages and environmental harm. Without requiring any modifications, biodiesel can be used straight to car engines to enhance engine combustion and lower negative emissions. This study's main goal is to give a general overview of how biodiesel applications affect diesel engines, considering how they affect emissions, vibration, noise levels, compatibility, engine performance and combustion characteristics. This study covers unregulated emissions, concentrating on two topics that have not been thoroughly studied: Polycyclic Aromatic Hydrocarbons (PAHs) and Volatile Organic Compounds (VOCs). This will be crucial that regulators, researchers and industry stakeholders understand the full spectrum of consequences associated with biodiesel as they collaborate to develop engine fuels in a sustainable and ethical manner.