Effects of Injection Schemes and Column Geometry on the Performance of Microchip Electrophoresis Devices

Jacobson, Stephen C.; Hergenröder, Roland; Koutny, Lance; Warmack, R. J.; Ramsey, J. Michael

doi:10.1021/ac00079a028

Cited by 747 publications

(686 citation statements)

References 12 publications

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“…Also, the use of micromachined channels on a chip has shown important possibilities for obtaining fast separations, i.e. of a few seconds, with high efficiencies (about 5000 theoretical plates per second) in miniaturized systems [10][11][12][13][14]. Nevertheless, the use of this sort of columns is actually not applied much in capillary electrophoresis because the disadvantages that they present compared with cylindrical columns.…”

Section: Introductionmentioning

confidence: 99%

Rectangular capillary electrophoresis: Some theoretical considerations

Cifuentes

Poppe

1994

Chromatographia

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SummaryA theoretical study of the use of rectangular columns in capillary electrophoresis (CE) is presented. It was done employing some of the most important parameters that normally define the performance of CE separations, i.e. thermal effect, analysis speed, efficiency and sample capacity. Theoretical results from rectangular and cylindrical capillaries are compared in terms of the aforementionated parameters. Also, an estimate of the additional zone broadening that arises from the noninfinite dimension in the y-direction, in which the channel has its largest dimension, is presented.

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Section: Introductionmentioning

confidence: 99%

Rectangular capillary electrophoresis: Some theoretical considerations

Cifuentes

Poppe

1994

Chromatographia

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“…Soon after, J. Michael Ramsey's group at Oak Ridge National Laboratory published its fundamental papers on µTAS (3,4), and the floodgates opened. What followed were reams of research papers on chip-based separations from laboratories around the world.…”

Section: Opening the Floodgatesmentioning

confidence: 99%

Shrinking the LC Landscape

Harris¹

2003

Anal. Chem.

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“…The common modes are pinched [8], floating [8], gated [9], and dynamic [10] injections. Pinched injection provides an accurate, precise and well-defined volume of sample solution with high separation efficiency, but the injection volume is limited by the dimensions of the microchip intersection, and the tight focus of the sample may be detrimental to detection sensitivity due to dilution and dispersion effects [11].…”

Section: Introductionmentioning

confidence: 99%

Study of injection bias in a simple hydrodynamic injection in microchip CE

et al. 2007

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The electrokinetically pinched method is the most commonly used mode for sample injection in microchip capillary electrophoresis (μCE) due to its simplicity and well-defined sample volume. However, the limited injection volume and the electrophoretic bias of the pinched injection may limit its universal usage to specific applications. Several hydrodynamic injection methods in μCE have been reported; however, almost all claimed that their methods are bias-free without considering the dispensing bias. To investigate the dispensing bias, a simple hydrodynamic injection was developed in single-T and double-T glass microchips. The sample flow was produced by hydrostatic pressure generated by the liquid level difference between the sample reservoir and the other reservoirs. The reproducibility of peak area and peak area ratio was improved to a significant extent by using largesurface reservoirs for the buffer reservoir and the sample waste reservoir to reduce the Laplace pressure effect. Without a voltage applied on the sample solution, the voltage-related sample bias was eliminated. The dispensing bias was analyzed theoretically and studied experimentally. It was demonstrated that the dispensing bias existed and could be reduced significantly by appropriately setting up the voltage configuration and by controlling the appropriate liquid level difference.

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Effects of Injection Schemes and Column Geometry on the Performance of Microchip Electrophoresis Devices

Abstract: A glass microchip column was fabricated for free-solution electrophoresis. The channels were wet chemically etched on a substrate using standard photolithographic techniques and were sealed using a direct bonding technique. Two methods * Chemical and Analytical Sciences Division.

Cited by 747 publications

References 12 publications

Rectangular capillary electrophoresis: Some theoretical considerations

Rectangular capillary electrophoresis: Some theoretical considerations

Shrinking the LC Landscape

Study of injection bias in a simple hydrodynamic injection in microchip CE

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