Effects of inoculation route and dose on production and avidity of IgY antibodies

Montini, Michele Pagliari Oliveira; Fernandes, Eduardo Vignoto; Ferraro, Ana Carolina Navarro dos Santos; Almeida, Mayara Alencar; Silva, Fabiano Cardoso da; Venâncio, Emerson José

doi:10.1080/09540105.2017.1376036

Cited by 5 publications

(6 citation statements)

References 31 publications

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“…Moreover, IgY antibody response to doses in the range of 1 to 100 ug of bovine serum albumin was proven to be similar from the sixth week after prime dose ( 57 ). Likewise, human IgG antigen doses of 2 ug, 20 ug, and 200 ug increased antibody levels in a similar way after the third immunization, supporting the importance of testing different antigen amounts to improve the cost-effectiveness of the method ( 58 ).…”

Section: Discussionmentioning

confidence: 76%

Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19

Agurto-Arteaga

Poma-Acevedo²,

Rios-Matos³

et al. 2022

Front. Immunol.

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Within the framework of the current COVID-19 pandemic, there is a race against time to find therapies for the outbreak to be controlled. Since vaccines are still tedious to develop and partially available for low-income countries, passive immunity based on egg-yolk antibodies (IgY) is presented as a suitable approach to preclude potential death of infected patients, based on its high specificity/avidity/production yield, cost-effective manufacture, and ease of administration. In the present study, IgY antibodies against a recombinant RBD protein of SARS-CoV-2 were produced in specific-pathogen-free chickens and purified from eggs using a biocompatible method. In vitro immunoreactivity was tested, finding high recognition and neutralization values. Safety was also demonstrated prior to efficacy evaluation, in which body weight, kinematics, and histopathological assessments of hamsters challenged with SARS-CoV-2 were performed, showing a protective effect administering IgY intranasally both as a prophylactic treatment or a post-infection treatment. The results of this study showed that intranasally delivered IgY has the potential to both aid in prevention and in overcoming COVID-19 infection, which should be very useful to control the advance of the current pandemic and the associated mortality.

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Section: Discussionmentioning

confidence: 76%

Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19

Agurto-Arteaga

Poma-Acevedo²,

Rios-Matos³

et al. 2022

Front. Immunol.

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“…Furthermore, inoculation via intramuscular, intradermal, and subcutaneous routes and the dose of the antigen do not seem to influence the avidity of the antibodies obtained [112].…”

Section: Other Factorsmentioning

confidence: 92%

“…In addition, other concentrations of urea can be used, such as 1 M [114] and 8 M [110]. For the use of magnesium chloride, two conditions are observed: incubation for 30 min after the incubations with the antibody samples [109,112,115], or the addition of magnesium chloride together with the antibody sample of magnesium chloride [101]. Ammonium thiocyanate was used in only one study, which determined the AI as the molarity of ammonium thiocyanate required for a 50% reduction in optical density relative to optical density without ammonium thiocyanate [76].…”

Section: Methodology For the Determination Of Affinity And Avidity Of...mentioning

confidence: 99%

“…Regarding the time, high avidity rates (60 to 75%) are observed within 30 days after the first immunisation [101][102][103][104][105][106], and in some studies, 100% avidity rates are observed between day 7 and 21 of the first immunisation [107,108]. On the other hand, other studies did not obtain antibodies with a high avidity index (below 60%) in this same period of time [109][110][111][112][113]. In addition, some studies were not able to produce antibodies with high avidity [114,115].…”

Section: Timementioning

confidence: 98%

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IgY Antibodies from Birds: A Review on Affinity and Avidity

Pacheco,

Nogueira,

Venancio

2023

Animals

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IgY antibodies are found in the blood and yolk of eggs. Several studies show the feasibility of utilising IgY for immunotherapy and immunodiagnosis. These antibodies have been studied because they fulfil the current needs for reducing, replacing, and improving the use of animals. Affinity and avidity represent the strength of the antigen–antibody interaction and directly influence antibody action. The aim of this review was to examine the factors that influence the affinity and avidity of IgY antibodies and the methodologies used to determine these variables. In birds, there are few studies on the maturation of antibody affinity and avidity, and these studies suggest that the use of an adjuvant-type of antigen, the animal lineage, the number of immunisations, and the time interfered with the affinity and avidity of IgY antibodies. Regarding the methodologies, most studies use chaotropic agents to determine the avidity index. Studies involving the solution phase and equilibrium titration reactions are also described. These results demonstrate the need for the standardisation of methodologies for the determination of affinity and avidity so that further studies can be performed to optimise the production of high avidity IgY antibodies.

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“…There is a great interest in the production of specific antibodies in laying hens for immunotherapy and immunodiagnostics to replace antibodies produced in mammalians (MONTINI et al, 2018;MARCQ et al, 2015;MARCQ et al, 2013;ETO et al, 2012). Laying hens represent an alternative to mammalians due to the possibility of obtaining large quantities of IgY from egg yolk at a low cost (MUNHOZ et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory action of jacalin from Artocarpus integrifolia and mannoprotein from Saccharomyces uvarum on the humoral immunity of laying hens

Silva

Pinheiro

et al. 2020

Cienc. Rural

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Advances in the fields of glycobiology and immunology have provided many insights into the role of carbohydrate-protein interactions in the immune system. Jacalin of Artocarpus integrifolia (JCA) and structural mannoprotein of Saccharomyces uvarum (MPS) are molecules with immunomodulatory properties. JCA is an IgA human lectin binding molecule that causes the mitogenic stimulation of immune cells, production of cytokines, chemotaxis, and activation of leukocytes. Studies on the immunomodulatory properties of JCA and MPS in mammals and fish suggest that they have an action on antibody production. The aim of this study was to investigate the possible action of JCA and MPS on the production of specific antibodies in laying hens. For this, laying hens were inoculated with an intra abdominal injection of sheep red blood cells (SRBC) with either JCA (0.075 µg, 0.75 µg, and 7.5 µg) or MPS (20 µg and 100 µg). Levels of anti-SRBC antibodies of the IgY, IgM, and IgA classes were evaluated by ELISA. Results showed that JCA and MPS have immunomodulatory effects on levels of anti-SRBC IgM, IgA, and IgY. An immunostimulatory effect of JCA was observed in primary immune response on anti-SRBC IgY, while an inhibitory effect of JCA and MPS was observed in secondary immune response on the production of IgM and IgA anti-SRBC. These results suggested that MPS and JCA have immunomodulatory effects on antibody production and could be used in future studies on humoral immune response in poultry.

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Effects of inoculation route and dose on production and avidity of IgY antibodies

Cited by 5 publications

References 31 publications

Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19

Preclinical Assessment of IgY Antibodies Against Recombinant SARS-CoV-2 RBD Protein for Prophylaxis and Post-Infection Treatment of COVID-19

IgY Antibodies from Birds: A Review on Affinity and Avidity

Immunomodulatory action of jacalin from Artocarpus integrifolia and mannoprotein from Saccharomyces uvarum on the humoral immunity of laying hens

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