Effects of Insulin-like Growth Factor-I Administration on Radiation Enteritis in Rats

Howarth, Gordon S.; Fraser, Robert; Frisby, Claudine L.; Schirmer, M; Yeoh, Eric

doi:10.3109/00365529709002990

Cited by 55 publications

(37 citation statements)

References 23 publications

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“…However, pre-and post-irradiation administration together do not provide superior protection compared to post-irradiation administration alone [129] . Administration of insulin-like g rowth factor (IGF)-I immediately following abdominal irradiation increases small-intestinal mass and improves indicators of mucosal integrity, suggesting acceleration of smallintestinal mucosal recovery from radiation injury [130] . More recently, growth hormone and IGF-I have been demonstrated to protect intestinal cells from radiationinduced apoptosis both in vitro, by inhibiting apoptosis of the cells and preserving the mucosal integrity [131] , and in vivo [132] , utilizing IGF-Ⅰ transgenic mice.…”

Section: Prevention Of Radiation Colitismentioning

confidence: 99%

Recent advances in the management of radiation colitis

Kountouras¹,

Zavos²

2008

WJG

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Section: Prevention Of Radiation Colitismentioning

confidence: 99%

Recent advances in the management of radiation colitis

Kountouras¹,

Zavos²

2008

WJG

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“…Colon histopathology was graded using the same method and the severity of damage was determined using the DSS (19). The four groups were examined in terms of 11 parameters, defined as colon surface epithelial erosion, severity of neutrophil leukocyte reaction in the lamina propria, severity of eosinophil reaction in the lamina propria, severity of nuclear changes, severity of fibrinoid changes in the vascular wall, severity of changes in fibroblasts in the mucosa, severity of lymphocyte reaction, mitosis, crypt abscess, blood vessel or lymphatic dilation, and ulcer form.…”

Section: Histopathologymentioning

confidence: 99%

Protective Effects of Quercetin on Intestinal Damage Caused by Ionizing Radiation

Pişkin¹,

Aydın²,

Baş³

et al. 2018

Haseki

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Radyasyona Bağlı İntestinal Hasarda Quercetin'in Koruyucu EtkisiAim: The aim of this study was to evaluate the protective use of quercetin in a rat model of radiation-induced enteritis and colitis.Methods: Twenty four adult rats were randomly divided into four groups. Group SHAM was given only physiological saline, group QUER was given quercetin 50 mg/kg for 15 days, group RAD was given only irradiation and group QUER+RAD was given quercetin 50 mg/kg, and then irradiated. Twenty four hours after the exposure to radiation, all rats were euthanized for the evaluation of the ileum and colon morphology and biochemical measurements. Results:Compared with the SHAM group, the serum malondialdehyde (MDA) level was significantly higher in group RAD (p=0.004) and was significantly decreased in group QUER+RAD (p=0.015). The MDA levels in the ileum and colon tissues were significantly higher in group RAD (p=0.004 and p=0.002, respectively), while treatment with quercetin significantly reduced lipid peroxidation in both tissues in group QUER+RAD (p=0.015 and p=0.009, respectively). Compared with the control group, the serum total antioxidant status (TAS) level was significantly lower in Group RAD (p=0.002) and was significantly increased in group QUER+RAD (p=0.009). TAS in the ileum and colon tissues were significantly lower in group RAD (p=0.002 and p=0.002) and were significantly higher in both tissues in group QUER+RAD (p=0.002 and p=0.002, respectively). Conclusion:This study confirmed that, in the model of radiationinduced ileitis and colitis in rats, quercetin effectively decreased oxidative stress and inflammatory damage to both ileum and colon tissues.Keywords: Quercetin, intestine damage, oxidative stress, radiation, rat Amaç: Bu çalışmanın amacı rat modelinde radyasyona bağlı oluşan enterit ve kolit tedavisinde quercetin'in koruyucu rolünü değerlendirmektir. Yöntemler:Yirmi dört yetişkin rat randomize olarak dört gruba ayrıldı. Group SHAM'a sadece salin, grup QUER'e 15 gün boyunca 50 mg/ kg quercetin, grup RAD'a sadece radyasyon ve grup QUER+RAD'a 15 gün boyunca 50 mg/kg quercetin ardından radyasyon uygulandı. Radyasyon maruziyetinden 24 saat sonra ileum ve kolon morfolojisi ve biyokimyasal parametrelerin değerlendirilmesi için ratlara ötenazi uygulandı. Bulgular: SHAM grubuyla karşılaştırıldığında, serum malondialdehit (MDA) seviyesi grup RAD'da istatistiksel olarak anlamlı derecede yüksek (p=0,004) ve grup QUER+RAD'da düşük bulundu (p=0,015). İleum ve kolon dokularındaki MDA seviyesi grup RAD'da anlamlı olarak daha yüksek (sırasıyla; p=0,004 ve p=0,002) idi. Grup QUER+RAD'da ise her iki dokuda azalmış olarak bulundu (sırasıyla; p=0,015 ve p=0,009). Kontrol grubuyla karşılaştırıldığı zaman, serum total antioksidan kapasite (TAK) seviyesi grup RAD'da istatistiksel olarak anlamlı derecede düşük (p=0,002) ve grup QUER+RAD'da ise yüksek bulundu (p=0,009). İleum ve kolon dokularındaki TAK seviyesi grup RAD'da istatistiksel olarak anlamlı derecede düşük iken (sırasıyla; p=0,002 ve p=0,002), grup QUER+RAD'da ise her iki ...

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“…Histopathological specimens were evaluated using the method defined previously with technical modification 23 . A blinded pathologist assessed the following 10 parameters: (1) presence of inflammatory infiltrate (inflammation) in the lamina propria, (2) leukocyte infiltration into crypt epithelium (cryptitis), (3) crypt distortion, (4) regenerative (reactive) atypia in epithelial cells, (5) crypt abscess, (6) mucosal ulcer, (7) epithelial degeneration, (8) mucosal edema, (9) mucosal thinning, and (10) reactive lymphnode hyperplasia.…”

Section: Tissue Collection and Histopathological Evaluationmentioning

confidence: 99%

L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats

et al. 2016

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PURPOSE:To evaluate histopathologically the radioprotective effect of L-carnitine on the colonic mucosa in rats undergoing abdominopelvic irradiation. METHODS:Thirty-two rats were randomly assigned to four experimental groups: intraperitoneal administration of normal saline (group 1) or L-carnitine (300 mL/kg; group 2), followed in groups 3 and 4, respectively, by one dose of abdominopelvic radiation (20 Gy) 30 min later. Rats were sacrificed 5 days after radiation, and their descending colons were resected for histopathological evaluation of the presence and severity of damage. RESULTS:Average damage scores did not differ significantly between groups 1 and 2 (0.13 ± 0.35 and 0.25 ± 0.46, respectively); the group 3 score was highest (10.25 ± 0.71), and the group 4 score (3.63 ± 1.41) was significantly lower than that of group 3 (both p = 0.0001). Pre-radiation L-carnitine administration significantly reduced mucosal thinning, crypt distortion, reactive atypia, inflammation, cryptitis, and reactive lymph-node hyperplasia (all p < 0.01).CONCLUSIONS: L-carnitine had a radioprotective effect on rat colonic mucosa. L-carnitine use should be explored for patients with gastrointestinal cancer, who have reduced serum L-carnitine levels.

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Effects of Insulin-like Growth Factor-I Administration on Radiation Enteritis in Rats

Abstract: IGF-I administration after abdominal irradiation increased small-intestinal mass and improved indicators of mucosal integrity, suggesting acceleration of small-intestinal mucosal recovery from radiation injury.

Cited by 55 publications

References 23 publications

Recent advances in the management of radiation colitis

Recent advances in the management of radiation colitis

Protective Effects of Quercetin on Intestinal Damage Caused by Ionizing Radiation

L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats

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