Nitrous oxide (N 2 O) emissions from agricultural soils, mainly caused by chemical nitrogen (N) fertilizer inputs, are major sources of N 2 O in Chinese terrestrial ecosystems. Thus, attempts to reduce N 2 O emissions from agricultural soils by optimizing N applications are receiving increasing attention. Further, organic fertilizers are being increasingly used in China to improve crop production/quality and prevent or reduce soil degradation. However, organic and chemical fertilizers are often both applied in spring in northeast China, which promotes N 2 O emissions and may be sub-optimal. Therefore, we hypothesized that reducing applications of chemical fertilizer N and applying manure in autumn could be an effective strategy for mitigating but not after manure inputs in autumn or during soil-thawing periods in the following spring. Emission factors for the chemical fertilizer N were on average 1.07% (1.00~1.10%) and 1.14% (0.49~1.83%) in 2010 and 2011, respectively. Furthermore, by comparing the nine pairs of fertilization treatments, the relative increase in cumulative nitrous oxide-nitrogen (N 2 O-N) emissions was found to be proportional to the relative increase in urea application, but independent of the amount of autumn-applied manure. These findings imply that N 2 O emissions from fertilized agricultural soils in northeast China could be mitigated by supplying manure in the autumn and reducing the total amount of chemical N fertilizer applied in the following year. Although no significant difference in maize grain yield was found among the fertilization treatments, the grain yield-scaled N 2 O emissions for the treatments with a lower chemical N application (e.g., N 230 M 15 and N 200 M 15 treatments) were significantly lower than those with a higher chemical N application (e.g., N 320 M 18 and N 280 M 18 treatments). Meanwhile, under the condition of the same application amount of chemical fertilizer N, the grain yield-scaled N 2 O emission decreased with the increase of manure application rate. Thus, the results support the hypothesis that combining reductions in chemical N fertilizer and applying manure in autumn could be an effective strategy for mitigating N 2 O emissions from N-fertilized soils in northeast China.