Effects of intense pulsed light on Cronobacter sakazakii inoculated in non-fat dry milk

Chen, Dongjie; Wiertzema, Justin; Peng, Peng; Cheng, Yanling; Liu, Juer; Mao, Qingqing; Ma, Yiwei; Anderson, Erik; Chen, Paul; Baumler, David J.; Chen, Chi; Vickers, Zata; Fęirtag, Joellen M.; Lee, Laurence; Ruan, Roger

doi:10.1016/j.jfoodeng.2018.06.022

Cited by 33 publications

(22 citation statements)

References 31 publications

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“…Considering quinones and many IPL-sensitive metabolites are constitutive components of bacterial cells, negative impacts of IPL on the metabolism and cultivability of treated bacteria in powdered food are expected. In fact, our recent studies have shown that the IPL treatment effectively inactivated Cronobacter sakazakii in non-fat dry milk and Bacillus cereus spores in mesquite pod flour [ 11 , 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…The successful adoption of the IPL platform in food processing and manufacturing will be largely dependent on its disinfection efficacy as well as its impacts on food quality. Chemometric analyses have been conducted to examine the effects of IPL on non-fat dry milk powder and mesquite pod flour [ 11 , 12 ]. The treatment did not significantly alter the color, amino acid composition, and the levels of volatile off-flavor compounds in milk powder [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Chemometric analyses have been conducted to examine the effects of IPL on non-fat dry milk powder and mesquite pod flour [ 11 , 12 ]. The treatment did not significantly alter the color, amino acid composition, and the levels of volatile off-flavor compounds in milk powder [ 11 ]. Mesquite pod flour retained the color and had reduced levels of volatile off-flavor compounds after the IPL treatment, though minor lipid oxidation was observed [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Despite its effectiveness, thermal pasteurization is known to alter the physical and chemical properties of food [ 5 ]. Other alternative sterilization technologies, including pulsed magnetic field [ 6 ], capillary discharge plasma [ 7 ], high hydrostatic pressure [ 8 ], high pressure carbon dioxide [ 9 ], and ultraviolet-C (UV-C) irradiation [ 10 ], have been developed for food manufacturing, but their applications in disinfecting the dry food with a low water activity (a w ), are restricted by their low efficiency and poor compatibility with industry practices [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…Intense pulsed light (IPL) is a novel non-thermal disinfection technology containing a broad spectrum of ultraviolet (UV), visible, and infrared light. A prototype IPL platform for the inactivation of microbes in low a w food was developed recently and its efficacy has been shown in disinfecting non-fat dry milk powder [ 11 ] and mesquite pod flour [ 12 ]. The IPL system inactivates microbes by delivering intensive energy to the target surface through pulsed flashing (1–20 flashes per second) of broad-spectrum light (190–1100 nm) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Quinone Degradation as a Triggering Event for Intense Pulsed Light-Elicited Metabolic Changes in Escherichia coli by Metabolomic Fingerprinting

et al. 2021

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Intense pulsed light (IPL) is becoming a new technical platform for disinfecting food against pathogenic bacteria. Metabolic changes are deemed to occur in bacteria as either the causes or the consequences of IPL-elicited bactericidal and bacteriostatic effects. However, little is known about the influences of IPL on bacterial metabolome. In this study, the IPL treatment was applied to E. coli K-12 for 0–20 s, leading to time- and dose-dependent reductions in colony-forming units (CFU) and morphological changes. Both membrane lipids and cytoplasmic metabolites of the control and IPL-treated E. coli were examined by the liquid chromatography–mass spectrometry (LC-MS)-based metabolomic fingerprinting. The results from multivariate modeling and marker identification indicate that the metabolites in electron transport chain (ETC), redox response, glycolysis, amino acid, and nucleotide metabolism were selectively affected by the IPL treatments. The time courses and scales of these metabolic changes, together with the biochemical connections among them, revealed a cascade of events that might be initiated by the degradation of quinone electron carriers and then followed by oxidative stress, disruption of intermediary metabolism, nucleotide degradation, and morphological changes. Therefore, the degradations of membrane quinones, especially the rapid depletion of menaquinone-8 (MK-8), can be considered as a triggering event in the IPL-elicited metabolic changes in E. coli.

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Section: Discussionmentioning

confidence: 99%