Effects of Intercropping between Morus alba and Nitrogen Fixing Species on Soil Microbial Community Structure and Diversity

Liu, Jiaying; Wei, Yawei; Du, Haitao; Zhu, Wenxu; Zhou, Yongbin; Yin, You

doi:10.3390/f13091345

Cited by 7 publications

(7 citation statements)

References 70 publications

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“…For example, mulberry/alfalfa intercropping increases the richness and diversity of bacterial community by affecting the content of soil total carbon, available potassium, and phosphate ( Zhang et al, 2018 ). Morus alba/Lespedeza bicolor intercropping significantly elevates the evenness and diversity of fungal community by affecting the contents of soil total carbon, nitrogen, and phosphate ( Liu J. et al, 2022 ). Correspondingly, our results also observed that maize/peanut intercropping system can significantly increase bacterial richness.…”

Section: Discussionmentioning

confidence: 99%

Intercropping enhances microbial community diversity and ecosystem functioning in maize fields

et al. 2023

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Background and aims:Intercropping, a widely used planting pattern, could affect soil physicochemical properties, microbial community diversity, and further crop yields. However, its impacts on soil microbial diversity and ecosystem functioning and further soil sustainability are poorly understood.Methods:We conducted field experiments by intercropping maize with four important crops (i.e., sesame, peanut, soybean, and sweet potato), and examined soil microbial community diversity and ecosystem functioning such as microbial biomass and enzyme activities under monocropping and intercropping. We quantified their intercropping effects on microbial diversity and ecosystem functions with effect size metric Cohen d by comparing to the monocropping of maize.Results:We found that the four intercropping systems significantly increased soil aggregates in respective of the 2–0.25 mm grain size. Intercropping consistently elevated ecosystem functioning, such as soil enzyme activities of urease, phosphatase, and catalase, soil microbial biomass carbon and soil microbial biomass nitrogen. The Cohen d of bacterial richness also increased from 0.39 to 2.36, the latter of which was significant for maize/peanut intercropping. Notably, these ecosystem functions were strongly associated with the diversity of bacteria and fungi and the relative abundance of their ecological clusters identified with network analysis.Conclusion:Together, our findings indicate that intercropping generally affected soil physicochemical properties, ecosystem functions, and promoted microbial community diversity. More importantly, our findings highlight the important roles of microbial diversity of ecological clusters (that is, network modules) in maintaining ecosystem functioning after intercropping. These results will help to better understand the microbial diversity and ecosystem function in intercropping systems and guide agricultural practice.

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Section: Discussionmentioning

confidence: 99%

Intercropping enhances microbial community diversity and ecosystem functioning in maize fields

et al. 2023

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“…Using fasttree2 and mafft, non-singleton amplicon sequence variants (ASVs) were used to create a phylogenetic tree ( Katoh, 2002 ; Price et al, 2009 ). Alpha-diversity metrics [Observed species richness ( Liu et al, 2022 ), Simpson index ( Simpson, 1949 ), and Pielou’s evenness index ( Pielou, 1966 )], beta diversity metrics (Bray-Curtis dissimilarity) were estimated using the diversity plugin. The SILVA Release 132 Database (Bacterium) and UNITE Release 8.0 Database (Fungus) were used as the sources for the naive Bayes taxonomy classifier in the feature-classifier plugin to assign taxonomy to ASVs ( Kõljalg et al, 2013 ; Bokulich et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Intercropping changed the soil microbial community composition but no significant effect on alpha diversity

Liu,

Zhang,

Teng

et al. 2024

Front. Microbiol.

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IntroductionEnhancing the planning of the forest-agricultural composite model and increasing the efficiency with which forest land is utilized could benefit from a thorough understanding of the impacts of intercropping between forests and agriculture on soil physicochemical properties and microbial communities.MethodsPopulus cathayana × candansis cv. Xinlin No.1 and Glycine max intercrop soils, along with their corresponding monocrops, were used in this study’s llumina high-throughput sequencing analysis to determine the composition and diversity of soil bacterial and fungal communities.ResultsThe findings indicated that intercropping considerably raised the soil’s total phosphorus content and significantly lowered the soil’s carbon nitrogen ratio when compared to poplar single cropping. Furthermore, the total carbon and nitrogen content of soil was increased and the soil pH was decreased. The sequencing results showed that intercropping had no significant effect on soil alpha diversity. Intercropping could increase the composition of fungal community and decrease the composition of bacterial community in poplar soil. At the phylum level, intercropping significantly increased the relative abundance of four dominant phyla, i.e., Patescibacteria, Proteobacteria, Patescibacteria and Deinococcus-Thermus. And the relative abundances of only two dominant phyla were significantly increased. It was found that soil total phosphorus and available phosphorus content had the strongest correlation with soil bacterial community diversity, and soil pH had the strongest correlation with soil fungal community diversity.DiscussionThe results of this study were similar to those of previous studies. This study can serve as a theoretical foundation for the development of a poplar and black bean-based forest-agricultural complex management system in the future.

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“…The sample was kept in a 100 mL volume bottle after digestion and cooling. The molybdenum-antimony resistance colorimetric method was used to perform colorimetric analysis on five mL of filtrate using a spectrophotometer (P4 UV-Visible China) and a 50 mL volumetric bottle ( Liu et al, 2022b ).…”

Section: Methodsmentioning

confidence: 99%

“…Consequently, it has a substantial influence on soil microbial community diversity and building processes ( Cheng et al, 2021 ). It has been shown that land use type explained 97% of the variability in soil quality indices and that different vegetation measures had significant effects on vegetation composition and structure, biomass, litter, soil moisture, soil nutrients and soil microorganisms of the ecosystem ( Xu et al, 2014 ; Zhang et al, 2011 ; Liu et al, 2022a ; Liu et al, 2022b ). Changes in vegetation type not only alter the landscape appearance of the land, but also the material cycle and energy flow of the soil ecosystem, while having a profound impact on the structure and function of soil microorganisms ( Wan & He, 2020 ; Zhang et al, 2013 ; Tian et al, 2017 ; Delgado-Baquerizo et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Similarities and differences in the microbial structure of surface soils of different vegetation types

Jiang,

Zhu,

Zhu

et al. 2023

PeerJ

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Background Soil microbial community diversity serves as a highly sensitive indicator for assessing the response of terrestrial ecosystems to various changes, and it holds significant ecological relevance in terms of indicating ecological alterations. At the global scale, vegetation type acts as a major driving force behind the diversity of soil microbial communities, encompassing both bacterial and fungal components. Modifications in vegetation type not only induce transformations in the visual appearance of land, but also influence the soil ecosystem’s material cycle and energy flow, resulting in substantial impacts on the composition and performance of soil microbes. Methods In order to examine the disparities in the structure and diversity of soil microbial communities across distinct vegetation types, we opted to utilize sample plots representing four specific vegetation types. These included a woodland with the dominant tree species Drypetes perreticulata, a woodland with the dominant tree species Horsfieldia hainanensis, a Zea mays farmland and a Citrus reticulata fields. Through the application of high-throughput sequencing, the 16S V3_V4 region of soil bacteria and the ITS region of fungi were sequenced in this experiment. Subsequently, a comparative analysis was conducted to explore and assess the structure and dissimilarities of soil bacterial and fungal communities of the four vegetation types were analyzed comparatively. Results Our findings indicated that woodland soil exhibit a higher richness of microbial diversity compared to farmland soils. There were significant differences between woodland and farmland soil microbial community composition. However, all four dominant phyla of soil fungi were Ascomycota across the four vegetation types, but the bacterial dominant phyla were different in the two-farmland soil microbial communities with the highest similarity. Furthermore, we established a significant correlation between the nutrient content of different vegetation types and the relative abundance of soil microorganisms at both phyla and genus levels. This experiment serves as a crucial step towards unraveling the intricate relationships between plants, soil microbes, and soil, as well as understanding the underlying driving mechanism.

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Effects of Intercropping between Morus alba and Nitrogen Fixing Species on Soil Microbial Community Structure and Diversity

Cited by 7 publications

References 70 publications

Intercropping enhances microbial community diversity and ecosystem functioning in maize fields

Intercropping enhances microbial community diversity and ecosystem functioning in maize fields

Intercropping changed the soil microbial community composition but no significant effect on alpha diversity

Similarities and differences in the microbial structure of surface soils of different vegetation types

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