Effects of intra-aortic balloon counterpulsation in a model of septic shock*

Solomon, Steven B.; Minneci, Peter C.; Deans, Katherine J.; Feng, Jing; Eichacker, Peter Q.; Banks, Steven M.; Danner, Robert L.; Natanson, Charles; Solomon, Michael A.

doi:10.1097/ccm.0b013e31818727bf

Cited by 20 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protocol followed in these experiments has been previously described (20, 21, 23) (Figure 1 and Supplementary Methods). At time 0 (T0), baseline blood samples and hemodynamic profiles were obtained (Supplementary Methods), and then animals received an inoculation of S. aureus (1.5 – 7.5 × 10 9 cfu/kg) into the right caudal lobe via bronchoscopy(20).…”

Section: Methodsmentioning

confidence: 99%

“…Using intrapulmonary challenges of Staphylococcus aureus to produce septic shock in canines (20, 21), stress doses of dexamethasone (DEX), a specific glucocorticoid agonist, or desoxycorticosterone (DOC), a specific mineralocorticoid agonist, were compared for effects on pathophysiology and survival. To simulate therapeutic interventions commonly employed in human septic shock, animals were managed with standardized sedation, antibiotic therapy, mechanical ventilation support adjusted to correct gas exchange abnormalities, and need-based cardiovascular support using vasopressors and fluids to normalize intravascular pressures.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficacy of selective mineralocorticoid and glucocorticoid agonists in canine septic shock

Hicks

Sweeney

Danner

et al. 2012

Critical Care Medicine

Self Cite

View full text Add to dashboard Cite

Background Corticosteroid regimens that stimulate both mineralocorticoid and glucocorticoid pathways consistently reverse vasopressor-dependent hypotension in septic shock, but have variable effects on survival. Objective and Methods To determine if exogenous mineralocorticoid and glucocorticoid stimulation have distinct effects, and whether the timing on such stimulation alters their effects, in septic shock. Desoxycorticosterone (DOC), a selective mineralocorticoid agonist; dexamethasone (DEX), a selective glucocorticoid agonist; and placebo were administered either several days before (prophylactic) or immediately after (therapeutic) infectious challenge and continued for 96 h in 74 canines with staphylococcal pneumonia. Measurements and Main Results Effects of DOC and DEX were different and opposite depending on timing of administration for survival (p=0.05); fluid requirements (p=0.05); central venous pressures (p≤0.007); indicators of hemoconcentration [i.e. sodium (p=0.0004), albumin (p=0.05), and platelet counts (p=0.02)]; IL-6 levels (p=0.04); and cardiac dysfunction (p=0.05). Prophylactic DOC treatment significantly improved survival, shock, and all the other outcomes above, but therapeutic DOC did not. Conversely, prophylactic DEX was much less effective for improving these outcomes compared to therapeutic DEX, with the exception of shock reversal. Prophylactic DEX given before sepsis induction also significantly reduced serum aldosterone and cortisol levels and increased body temperature and lactate levels compared to therapeutic DEX (p≤0.05), consistent with adrenal suppression. Conclusions In septic shock, mineralocorticoids are only beneficial if given prophylactically, while glucocorticoids are most beneficial when given close to the onset of infection. Prophylactic mineralocorticoids should be further investigated in patients at high risk to develop sepsis, whereas glucocorticoids should only be administered therapeutically to prevent adrenal suppression and worse outcomes.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of selective mineralocorticoid and glucocorticoid agonists in canine septic shock

Hicks

Sweeney

Danner

et al. 2012

Critical Care Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Intra-aortic balloon pumping (IABP) is expected to increase the cardiac output and reduce the dosage of a vasopressor. Solomon et al demonstrated that in a canine model of severe septic shock with a low cardiac index, IABP prolongs survival time and lowers vasopressor requirements [ 52 ]. Nakamura et al reported two cases of severe sepsis-induced cardiomyopathy with refractory shock [ 53 ].…”

Section: Reviewmentioning

confidence: 99%

A review of sepsis-induced cardiomyopathy

Sato¹,

Nasu²

2015

j intensive care

243

219

View full text Add to dashboard Cite

Sepsis-induced cardiomyopathy is a reversible myocardial dysfunction that typically resolves in 7–10 days. It is characterized by left ventricular dilatation and depressed ejection fraction. However, many uncertainties exist regarding the mechanisms, characteristics, and treatments of this condition. Therefore, this review attempts to summarize our current knowledge of sepsis-induced cardiomyopathy.

show abstract

“…Healthy canine kidney tissue was provided by the National Institutes of Health (NIH) Division of Veterinary Services. Kidneys were obtained from a healthy 2-year-old male Beagle (Charles River Laboratories, Frederick, MD) used in an Institutional Animal Care and Use Committee (IACUC)-approved study of the effects of intra-aortic balloon counterpulsation on septic shock (Solomon et al 2009 ). Histopathological evaluation of formalin-fixed tissue sections from the specimen was unremarkable.…”

Section: Methodsmentioning

confidence: 99%

A novel canine kidney cell line model for the evaluation of neoplastic development: karyotype evolution associated with spontaneous immortalization and tumorigenicity

et al. 2015

View full text Add to dashboard Cite

The molecular mechanisms underlying spontaneous neoplastic transformation in cultured mammalian cells remain poorly understood, confounding recognition of parallels with the biology of naturally occurring cancer. The broad use of tumorigenic canine cell lines as research tools, coupled with the accumulation of cytogenomic data from naturally occurring canine cancers, makes the domestic dog an ideal system in which to investigate these relationships. We developed a canine kidney cell line, CKB1-3T7, which allows prospective examination of the onset of spontaneous immortalization and tumorigenicity. We documented the accumulation of cytogenomic aberrations in CKB1-3T7 over 24 months in continuous culture. The majority of aberrations emerged in parallel with key phenotypic changes in cell morphology, growth kinetics, and tumor incidence and latency. Focal deletion of CDKN2A/B emerged first, preceding the onset and progression of tumorigenic potential, and progressed to a homozygous deletion across the cell population during extended culture. Interestingly, CKB1-3T7 demonstrated a tumorigenic phenotype in vivo prior to exhibiting loss of contact inhibition in vitro. We also performed the first genome-wide characterization of the canine tumorigenic cell line MDCK, which also exhibited CDKN2A/B deletion. MDCK and CKB1-3T7 cells shared several additional aberrations that we have reported previously as being highly recurrent in spontaneous canine cancers, many of which, as with CDKN2A/B deletion, are evolutionarily conserved in their human counterparts. The conservation of these molecular events across multiple species, in vitro and in vivo, despite their contrasting karyotypic architecture, is a powerful indicator of a common mechanism underlying emerging neoplastic activity. Through integrated cytogenomic and phenotypic characterization of serial passages of CKB1-3T7 from initiation to development of a tumorigenic phenotype, we present a robust and readily accessible model (to be made available through the American Type Culture Collection) of spontaneous neoplastic transformation that overcomes many of the limitations of earlier studies.Electronic supplementary materialThe online version of this article (doi:10.1007/s10577-015-9474-8) contains supplementary material, which is available to authorized users.

show abstract

Effects of intra-aortic balloon counterpulsation in a model of septic shock*

Abstract: Background-Fluid refractory septic shock can develop into a hypodynamic cardiovascular state in both children and adults. Despite management of these patients with empiric inotropic therapy (with or without a vasodilator), mortality remains high.

Cited by 20 publications

References 27 publications

Efficacy of selective mineralocorticoid and glucocorticoid agonists in canine septic shock

Efficacy of selective mineralocorticoid and glucocorticoid agonists in canine septic shock

A review of sepsis-induced cardiomyopathy

A novel canine kidney cell line model for the evaluation of neoplastic development: karyotype evolution associated with spontaneous immortalization and tumorigenicity

Contact Info

Product

Resources

About