Plant density is one of the most important factors influencing the physiological, morphological characteristics, and yield of sesame. Sesame is an important oilseed and cash crop production in Sudan. The effects of different rows spacing on the growth attributes, yield and yield compound on three sesame seeds varieties were investigated at rainfall conditions in semi-arid regions. Two field experiments were conducted in 2014-2015 and 2015-2016. Three varieties were planted at four rows spacing [5(control), 10, 20 and 30cm]. The experiment was laid out in RCBD in split plots with three replications. There were significant differences between row spacing, varieties and interaction between row spacing and varieties for all parameters except the 1000 seeds weight. Moreover, Abo Nama has recorded the highest plants, number of branches and number of capsules per plant, while the abo Radom recorded the highest number of seeds per capsule and seed yield, and abo sofa had the highest weight of 1000 seeds. Planted varieties at the 5 cm between rows can increase the seed yield (210.18 kg/h). The interaction between Abo Radom and control recorded the high seed yield by 265.30 kg per h-1. This study suggests that the appropriate rows spacing can actively increase the yield of the sesame plant.