Effects of intracerebroventricularly administered octreotide on gonadotrophic cells in female rats

Abstract

Effects of intracerbroventricularly (ICV) administered octreotide on gonadotrophic cells (FSH and LH) of adult Wistar female rats were examined by immunocytochemical and morphometric methods. The animals received ICV three 1.0 mg doses of octreotide dissolved in 10 mL saline every second day. The controls were treated with equivalent volume of physiological saline by the same schedule. FSH-and LH-producing cells were examined using peroxidase-antiperoxidase immunocytochemical procedure. Morphometric and stereologic examinations were performed to evaluate changes in the number, volume and volume densities of gonadotrophic cells. In females treated with octreotide, the gonadotrophic cells were smaller and often pycnotic, while the number of FSH-and LH-immunopositive cells per unit area (mm 2) was significantly reduced. Octreotide also induced a significant reduction of the FSH-and LH-immunoreactive cells volume, as well as of their volume densities. On the basis of these results it can be concluded that octeotride, centrally administered to adult female rats provokes changes in immunocytochemical and morphometric features of both types of gonadotrophic cells.

Prenatal Glucocorticoids: Short-Term Benefits and Long-Term Risks

“…For example, an impaired somatotropic axis negatively affects reproduction. Somatostatin treatment inhibits pituitary gonadotropic cells and initial folliculogenesis in the ovaries of infant, peripubertal and adult females [252][253][254][255][256][257]. Polycystic ovary syndrome (PCOS) is of great importance, owing to its prevalence in up to 10% of the women population of reproductive age.…”

Long-term effects of somatostatin 14 on the pituitary-ovarian axis in rats

“…Ben-Shlomo and Melmed (2010) suggested that this effect is probably mediated through induction of cell apoptosis or induction of cell senescence, rather than inhibition of mitogenesis. Indeed, pycnotic gonadotropic cells were observed after acute central octreotide administration (Nestorović et al 2004b). …”

Somatostatin in the Periventricular Nucleus of the Female Rat: Age Specific Effects of Estrogen and Onset of Reproductive Aging

“…(Estupina et al, 1996, Zorrila et al, , 1991. Central injections with SOM or a SOM analog (octreotide) decreased the number of gonadotropic cells in the pituitary (Lovren et al, 1998;Nestorovic et al 2002;. Also, we previously showed that a single central injection with octreotide significantly attenuated the E 2 -induced Luteinizing Hormone (LH) surge and significantly decreased the activation of Gonadotropin Releasing Hormone (GnRH) cells in the hypothalamus of female rats (van Vugt et al, 2004).…”