Effects of intraocular miotics on cultured bovine corneal endothelium.

Abstract

Experiments were set up to study the morphological changes induced in bovine corneal endothelial cell culutures in vitro after a brief exposure to various miotic preparations. The relative toxic effects of pH, osmolality, absence of calcium, or presence of pilocarpine were investigated. The probable cause of the corneal oedema noted in the 2 clinical cases included in this report was identified, and the minimum requirements for the formulation of a miotic solution for intraocular use are outlined. Case reports CASE IAn apparently uncomplicated intracapsular cryoextraction for presenile nuclear cataract was performed on a 51-year-old woman. Preoperative examination of the cornea by specular reflection with the slit-lamp microscope hiad shown slight

Corneal toxicity secondary to inadvertent use of benzalkonium chloride preserved viscoelastic material in cataract surgery

“…Various agents have been proved to have a toxic effect on the human or animal corneal endothelium. [1][2][3][4][5][6][7][8][9] Endothelial toxicity is related to the chemical composition, 10 11 concentration, pH, 12 and osmolality 13 of substances (irrigating solutions and drugs) that come into contact with the endothelium and it may lead to, often irreversible, corneal oedema. Resolution of the corneal oedema depends on the density and function of the remaining endothelial cells.…”

Corneal endothelial protection during manual small-incision cataract surgery

“…[ 54 ] AC reformation at the end of cataract surgery with a sterile solution of 1% pilocarpine nitrate in water has been associated with the development of dense corneal edema and cornea guttate changes. [ 55 ] Studies published on the use of intracameral pilocarpine in lower concentration (0.2% by Sethi et al . [ 56 ] and 0.13 mg/mL by Wutthiphan et al .…”