Effects of intratesticular administration of zinc gluconate and dimethyl sulfoxide on clinical, endocrinological, and reproductive parameters in dogs

Vannucchi, Camila Infantosi; Angrimani, Daniel de Souza Ramos; Eyherabide, Ana Rodrigues; Mazzei, Claudia; Lúcio, Cristina de Fátima; Maiorka, Paulo César; Silva, L. C. G; Nichi, Marcílio

doi:10.1016/j.theriogenology.2015.06.005

Cited by 19 publications

(20 citation statements)

References 19 publications

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“…Based on the conditions used in this study, we conclude that calcium chloride associated with DMSO can be used as a method of chemical sterilization in bovines. Testicle, histology, serum testosterone, bulls, chemical castration, calcium chloride, dimethyl sulfoxide, sclerosing agent, seminiferous tubule, testosterone. with intratesticular injections (Vannucchi et al 2015) or epididymis tail (Navarrete 1997). Moreover, DMSO has some pharmacological actions, especially analgesic, anti-inflammatory and increases the diffusion of other drugs throughout tissues, which contribute to the chemical castration effect (Pearson et al 1980, Penasso 2005, Vannucchi et al 2015.…”

Section: Introductionmentioning

confidence: 99%

Testicular histological evaluation and serum testosterone concentrations of bulls after chemical castration with calcium chloride

et al. 2018

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Orchiectomized bulls have advantages in the meat quality and ease of handling. Chemical castration is an option for surgical castration and the sclerosing agents can be administered into the testicular or epididymis parenchyma. These agents have a lower incidence of complications than surgery, especially when associated with dimethylsulfoxide (DMSO), which has anti-inflammatory action and increases the absorption of other drugs. Thus, this study aimed to evaluate the effect of a single intratesticular injection of calcium chloride solution associated with DMSO for the chemical sterilization of bulls. Twenty-four young adult bulls were utilized, distributed into 3 groups (G20, G30 and G40, n = 8/group), according to the calcium chloride concentration (20, 30 and 40%), in 10mL volume. Serum concentrations of testosterone, body weight, testicular volume and ecotexture, clinical signs and behavior and were evaluated for 45 days. Thus, the animals were orchiectomized and testicles were assessed histologically. There were no changes in body weight, decreased serum testosterone concentrations (except G30), signs of scrotal sensitivity or changes in behavior over the period. However, there was significant increase in testicular volume, especially on the 2nd and 3rd day after treatment, with values returning to the value initials at 15 days. Testicular adherence and firm consistency were observed during orchiectomy. Ultrasound examination revealed a loss of integrity of the median raphe, with cavity formation and an alteration of the testicular echotexture. In the histological evaluation, coagulation necrosis of seminiferous tubules and interstitial cells was observed, mainly in the medial portion in all groups. Some animals presented total absence of tubular formations in all the studied groups, being the effects of greater intensity in the G40. Additionally, pronounced edema was noted in all groups, especially in G40. Inflammatory infiltrate, fibroplasia and neovascularization were found to be predominantly discrete. Based on the conditions used in this study, we conclude that calcium chloride associated with DMSO can be used as a method of chemical sterilization in bovines.

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Section: Introductionmentioning

confidence: 99%

Testicular histological evaluation and serum testosterone concentrations of bulls after chemical castration with calcium chloride

et al. 2018

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“…For the calculation of the carcass composition, 30 left half carcasses from each treatment were boned in anatomical cuts and dissected according to the method described by Walstra and Merkus (1996). The skin lesion scores were obtained visually in the shank, palette, and body of the carcasses, based on a standard guide of photographs (0= absence, 1= light, 2= moderate, 3= severe).…”

Section: Methodsmentioning

confidence: 99%

“…One of these attempts involves the intratesticular application of solutions containing neutralized zinc gluconate (Vannucchi et al, 2015), which is regarded as being a non-surgical sterilant (NSS) of a low cost and easy application (Cavalieri et al, 2015).…”

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confidence: 99%

Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

Lucas

Siqueira

Haguiwara

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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A non-surgical sterilant (NSS) was used with the objective of assessing the zootechnical performance, carcass and meat quality, and hormone levels. 90 male piglets were selected with birthweights varying between 1.5kg to 2.0kg. The control group was constituted of 45 males castrated by the conventional surgical method, at the seventh day of age, and the treated group was constituted of 45 males castrated with NSS (active principle of zinc gluconate in the concentration of 26.2mg/mL associated to the dimethyl sulfoxide at 0.5%), with the application of the first dose on the seventh day of age and the second dose on the fourteenth day of age. The zootechnical assessments were carried out on the farm and consisted: weight gain in the periods per animal and feed conversion. The slaughter of the animals and the assessment of the carcass and meat quality and hormone levels were carried out in a slaughterhouse and the municipality of Campinas (SP). The study demonstrated that the use of NSS was a viable alternative in relation to the zootechnical performance and the carcass quality. In relation to the meat quality there was no significant difference in the majority of the assessed parameters.Keywords: swine, zinc gluconate, androstenone, feed conversion, carcass yield RESUMO Foi utilizado esterilizante não cirúrgico (ENC) com

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“…While Tepsumethanon et al, (2005) documented histopathological finding that virtually complete with fibrosis of seminiferous tubules and Leydig cells occurs on days 60 and 75 post injection. Vannucchi et al, (2015) reported that intratesticular injection of zinc gluconate associated with DMSO, two months after resulted in reduction of testicular volume and changes of testicular echotexture including degeneration, fibrosis, and calcification of testicular parenchyma on histologic examination.…”

Section: Control Dogsmentioning

confidence: 99%

“…Similar finding was reported by Fahim et al, (1993) in adult dogs who observed no changes in cellular structure of testes of treated dogs, one year after intra epididymal injection of zinc arginine. However, Vannucchi et al, (2015) contradicted and concluded that impairment of sperm quality-related variables was observed 15 days after the first intra-testicular administration of zinc gluconate and dimethyl sulfoxide (i.e., decrease in sperm count and sperm motility and an increase in major sperm defects and by this a decrease in the total number of morphologically normal and motile sperm).…”

Section: Control Dogsmentioning

confidence: 99%

Histopathological Studies on Intra Epididymal Zinc Arginine Administration in Pre-Pubertal Dogs

Chaudhary¹,

Sharma²,

Mehra³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Effects of intratesticular administration of zinc gluconate and dimethyl sulfoxide on clinical, endocrinological, and reproductive parameters in dogs

Cited by 19 publications

References 19 publications

Testicular histological evaluation and serum testosterone concentrations of bulls after chemical castration with calcium chloride

Testicular histological evaluation and serum testosterone concentrations of bulls after chemical castration with calcium chloride

Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

Histopathological Studies on Intra Epididymal Zinc Arginine Administration in Pre-Pubertal Dogs

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