Effects of Intravenous Infusion With Sodium Butyrate on Colonic Microbiota, Intestinal Development- and Mucosal Immune-Related Gene Expression in Normal Growing Pigs

Chen, Xue; Xu, Jumei; Su, Yong; Zhu, Weiyun

doi:10.3389/fmicb.2018.01652

Cited by 20 publications

(17 citation statements)

References 71 publications

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“…However, our study showed the opposite result that the change in gut microbiota composition significantly decreased LPS content in chyme in high gelatinized starch diet group, compared with control group, which was beneficial to the gut health to a certain extent. Butyrate is an important energy source for gut enterocytes (Chen et al, 2018), and it is mainly from butyric acid-producing bacteria in gut (Hamer et al, 2008). Lack of butyric acid can result in gut permeability increase (Wu et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, many studies have focused on how the nutrients in the diet affect gut health or body health by reshaping the gut microbiota. Resistant starch in diet give rise to substantial changes in the microbiome and in fermentation products (Kleessen et al, 1997;Wang et al, 2002;Warren et al, 2018), and these fermentation products help improve the immune system (Chen et al, 2018). High levels of fat in diet change the gut microbial community, particularly by increasing the ratio of Firmicutes to Bacteroidetes to affect health of the host (Okazaki et al, 2016), and high-fat diets result in obesity and inflammation by destroying the structure of the gut microbiota (De Lartigue et al, 2011;Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effects of High Carbohydrate Diet-Modulated Microbiota on Gut Health in Chinese Perch

Zhang

Liang

et al. 2020

Front. Microbiol.

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High carbohydrate diet-induced damage in gut is linked to changes in gut permeability and microbiota. However, the mechanisms of action are not clear, especially in nonmammals. We performed the gut microbiota profiling in Chinese perch fed with different content of starch diets (0, 10, and 20%) by 16S rRNA sequencing. The gut permeability, metabolites, histological analysis, and inflammatory infiltration were evaluated. We found that gut microbial diversity, beneficial bacteria quantity, and lactic acid content were higher in C10 group than in the other groups. The lower level of gut microbial diversity was observed in C20 group, and mycoplasma was the overwhelmingly dominant species, but the butyric acid-producing bacteria and butyric acid level were significantly reduced. The gut permeability in C20 group was also increased due to the decreased mRNA expression levels of tight junction proteins caused by the butyric acid deficiency and gut lipid droplets accumulation. Then a large amount of LPS penetrated into the plasma, resulting in inflammation. These results suggested that high carbohydrate dietinduced damage in gut could be attributed to the endotoxemia, permeability, and gut microbiota, especially the role of mycoplasma and butyric acid-producing bacteria. In addition, predictive functional profiling of microbial communities by PICRUSt showed that C10 group enriched pathway related to membrane transport and down-regulated the pathways related to energy, coenzyme factor and vitamin metabolism, while C20 group exhibited reversed results. These data showed that the high-carbohydrate diet reversed the beneficial changes in gut microbial metabolism resulted from the mediumcarbohydrate diet, and further demonstrated that microbiota played a key role in the gut damage caused by the high-carbohydrate diet. Our findings provide a reference for the targeted regulation of gut microbiota to mitigate the damage caused by the increase in starch content in fish feed (cost saving).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of High Carbohydrate Diet-Modulated Microbiota on Gut Health in Chinese Perch

Zhang

Liang

et al. 2020

Front. Microbiol.

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“…Butyrate increased the expression of claudin‐3, claudin‐4 and restored the reduction in the abundance of these proteins caused by LPS challenge (Yan and Ajuwon ). Chen et al () reported that parenteral butyrate treatment increased the mRNA expression of ZO‐1 and occludins in the colon mucosa of piglets. Sodium butyrate improved the intestinal barrier function and maintained the intestinal mucosa intact (Fang et al ).…”

Section: Discussionmentioning

confidence: 99%

The effects of dietary sodium butyrate supplementation on the growth performance, carcass traits and intestinal microbiota of growing‐finishing pigs

Sun

et al. 2020

J Appl Microbiol

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Aim This study was carried out to investigate the effects of dietary sodium butyrate supplementation on growth performance, carcass traits and intestinal of growing‐finishing pigs. Methods and Results Thirty pigs (27·4 ± 0·4 kg) were randomly assigned to receive one of three diets: basal diet (negative control group), basal diet + 40 ppm zinc bacitracin (positive control group) and basal diet + 0·2% sodium butyrate (sodium butyrate group), respectively. The experiment lasted for 69 days, including 3 days for diet and housing condition adaptation. On day 70, five piglets from each diet group were slaughtered for collecting blood and tissue samples. When compared to the control group, final body weight, daily body weight gain and daily feed intake of pigs in the sodium butyrate group were increased (P < 0·05) and feed intake/body weight gain ratio was decreased (P < 0·05). Carcass weight of pigs in the sodium butyrate group was higher than that of pigs in the negative and positive groups (P < 0·05); backfat thickness of pigs in the positive group was higher than that of pigs in the negative group and sodium butyrate group (P < 0·001). When compared to the negative and positive groups, pigs fed diet supplemented with sodium butyrate showed a increased relative abundance of bacteroidetes in the caecum and a decreased relative abundance of fiemicutes and proteobacteria in the caecum (P < 0·05). Conclusion The results indicated that dietary sodium butyrate supplementation increased growth performance of growing‐finishing pigs and improved the carcass traits and intestinal health. Significance and Impact of the Study Antibiotic‐free feed has become an inevitable worldwide trend. This study showed that dietary sodium butyrate supplementation improved the growth performance and intestinal health of growing‐finishing pigs. Thus, sodium butyrate can be applied in growing‐finishing pig feed as an alternative of antibiotics.

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“…This agrees with the previous studies. Parenteral supplemented with sodium butyrate in a manner of intravenous infusion also significantly decreased the gene expression of colonic proinflammatory cytokines IL-6, IL-18, IL-12p40, and TNF-α, but increased the expression of intestinal development-related genes ZO-1 , occluding, and EGF ( Chen et al, 2018b ). Moreover, butyrate ameliorated Clostridium difficile -induced colonic inflammatory responses as indicated by significantly decreased inflammatory cytokines such as IL-1β, IL-6, and CXCL-1 ( Fachi et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Butyrate Mitigates Weanling Piglets From Lipopolysaccharide-Induced Colitis by Regulating Microbiota and Energy Metabolism of the Gut–Liver Axis

et al. 2020

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Inflammatory bowel disorder is accompanied by the destruction of immunity homeostasis, gut microbiota perturbation, and chronic inflammatory liver diseases. Butyrate is known as a primary energy source for colonocytes and functional substances for mitigating pathological features of colitis. However, it is still unclear whether butyrate alleviates colitis progression by regulation of microbiota and metabolism in the gut–liver axis. In the present study, we aimed to determine the role of microbiota and metabolism of the gut–liver axis in ameliorating lipopolysaccharide (LPS)-induced colitis in piglets using protected butyrate administration. Eighteen crossbred male piglets were weaned at 30 days old and were randomly allocated to three treatments, with CON (basal diet), LPS (basal diet + LPS), and BT-LPS (basal diet + 3.0 g/kg protected butyrate + LPS). On days 19 and 21, piglets in the LPS and BT-LPS groups were intraperitoneally challenged with LPS at 100 μg/kg body weight. Butyrate administration significantly decreased LPS-induced rise in the clinical score of piglets and colonic histological scores and reduced the susceptibility to LPS-induced severe inflammatory response by decreasing proinflammatory (IL-1β, IL-6, IL-8, and TNF-α) cytokines. Butyrate supplementation accelerated the prevalence of Faecalibacterium and Lactobacillus by enhancing the tricarboxylic acid (TCA) cycle of colonocytes. Dietary supplementation with protected butyrate significantly targeted increased concentrations of butyric acid in the colon and portal venous circulation, and enhanced the TCA cycle in the gut–liver axis by mobilizing amino acid and vitamin B group as a coenzyme. Meanwhile, during this progress, LPS increased fatty acid synthesis that was reversed by butyrate treatment, which was reflected by decreased acylcarnitines. Butyrate-reshaped colonic microbial community and metabolism in the gut–liver axis contributed to morphology integrity and immunity homeostasis by promoting anti-inflammatory (IL-10 and TGF-β) cytokines and suppressing inflammatory mediator hypoxia-inducible factor 1α and its downstream response elements cyclooxygenase 2 and inducible nitric oxide synthase. These results identified the pivotal role of colonic microbiota and metabolism in the gut–liver axis for alleviating inflammatory progression and possible therapeutic targets.

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Effects of Intravenous Infusion With Sodium Butyrate on Colonic Microbiota, Intestinal Development- and Mucosal Immune-Related Gene Expression in Normal Growing Pigs

Cited by 20 publications

References 71 publications

Effects of High Carbohydrate Diet-Modulated Microbiota on Gut Health in Chinese Perch

Effects of High Carbohydrate Diet-Modulated Microbiota on Gut Health in Chinese Perch

The effects of dietary sodium butyrate supplementation on the growth performance, carcass traits and intestinal microbiota of growing‐finishing pigs

Butyrate Mitigates Weanling Piglets From Lipopolysaccharide-Induced Colitis by Regulating Microbiota and Energy Metabolism of the Gut–Liver Axis

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