Effects of intravitreal triamcinolone acetonide on retinal gene expression in a rat model of central retinal vein occlusion

Abstract

Triamcinolone exerts anti-inflammatory effects in the ischemic retina by inhibitory effects on the gene expression of IL-1ß and IL-6, and may have neuroprotective effects via improvement of retinal potassium homeostasis.

Effect of Intravitreal Anti-Vascular Endothelial Growth Factor Treatment on the Retinal Gene Expression in Acute Experimental Central Retinal Vein Occlusion

“…On the other hand, intravitreally applied VEGF inhibitors have apparently no improving effect on the fluid clearance mediated by glial cells, as previously suggested for triamcinolone acetonide [16] . The extrapolation of the present results to clinical conditions should be done with caution.…”

“…The cDNA was diluted by the addition of 20 l RNase-free water. Semiquantitative real-time RT-PCR was performed with the Single-Color Real-Time PCR Detection System (BioRad, Munich, Germany) using the primer pairs described previously [16] . The PCR solution contained 1 l cDNA, specific primer set (0.25 M each) and 10 l of QuantiTect SYBR Green PCR Mix (Qiagen, Hilden, Germany) in a final volume of 20 l. The PCR parameters were: initial denaturation and enzyme activation (1 cycle at 95 ° C for 15 min); denaturation, amplification and quantification (45 cycles at 95 ° C for 30 s, 58 ° C for 20 s, and 72 ° C for 45 s); melting curve, 55 ° C with the temperature gradually increased (in increments of 0.5 ° C) up to 95 ° C. The amplified samples were analyzed by standard agarose gel electrophoresis.…”

Aldosterone Exposure Causes Increased Retinal Edema and Severe Retinopathy Following Laser-Induced Retinal Vein Occlusion in Mice

“…Several groups have used laser induced vein occlusion in mouse and rat to characterize the response of various retinal cell types following vein occlusion. [11][12][13][14][15][16][17] Most have occluded half or all retinal veins, which leads to extensive ischemia and eventual retinal neovascularization. We altered this approach by occluding only a single vein and examining the tissue in the peripheral retina that is drained by the occluded vein.…”

Animal Models of Retinal Vein Occlusion

“…In most cases, experimental RVO has been induced by traumatizing one or more retinal veins using laser photocoagulation. [67][68][69][70][71][72][73][74][75][80][81][82][83][84][85][86][87][88][89][90][91][92]96,97,101,[104][105][106][107][108][109][110][111][115][116][117][118][125][126][127][129][130][131][132][133][134][135][136][137][138][139]…”