Effects of ionic and nonionic contrast media on in vitro and in vivo platelet activation

Albanese, James R.; Venditto, John; Patel, Gaurang; Ambrose, John A.

doi:10.1016/s0002-9149(99)80297-5

Cited by 25 publications

(3 citation statements)

References 22 publications

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“…Platelet aggregation coupled with degranulation are important mechanisms involved in arterial thrombosis and consequently the effects of contrast media on platelet function have been investigated. Some reports suggest that non‐ionic low‐osmolar contrast media can cause degranulation ( Chronos et al , 1993 ; Hardeman et al , 1994 ; Van Beek et al , 1994 ; Albanese et al , 1995 ; Koza et al , 1995 ; Grabowski et al , 1995 ; Corot et al , 1996 ; Hay & Bull, 1995), which, through release of aggregating agents stored in intracellular granules, would be expected to be associated with platelet aggregation. However, the situation is confused by other reports that suggest that some contrast media inhibit platelet aggregation ( Gafter et al , 1979 ; Dawson et al , 1986 ; Taylor et al , 1991 ; Carr et al , 1995 ; Tamura et al , 1995 ).…”

mentioning

confidence: 99%

Differential effects of three radiographic contrast media on platelet aggregation and degranulation: implications for clinical practice?

et al. 1998

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Summary.We have determined the effects of three radiographic contrast media on platelet aggregation and degranulation in vitro. Aggregation was measured as loss of single platelets, and degranulation was measured as P-selectin expression using flow cytometry. Iopamidol added to hirudinized blood induced aggregation directly and also potentiated that induced by weak platelet agonists such as adenosine diphosphate (ADP). Iodixanol also potentiated platelet aggregation, but ioxaglate inhibited it. Iopamidol also caused marked platelet degranulation. The pro-aggregatory effect of iopamidol was evident in non-anticoagulated blood as well as in hirudinized blood, but not in citrated blood. In platelet-rich plasma (PRP) prepared from hirudinized blood neither iopamidol nor iodixanol directly induced platelet aggregation, but they rendered platelets hypersensitive to ADP. ADP antagonists inhibited the platelet aggregation and degranulation induced by iopamidol in whole blood, whereas aspirin, an inhibitor of thromborane A 2 synthesis, did not. These data are consistent with clinical reports of increased thromboembolic risk with non-ionic low-osmolar media, and raise concerns about the routine use of these contrast media during diagnostic and interventional arteriographic procedures. Routine use of citrate in previous experiments may have masked a pro-aggregatory effect of some contrast media.

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confidence: 99%

Differential effects of three radiographic contrast media on platelet aggregation and degranulation: implications for clinical practice?

et al. 1998

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“…Our results withioxaglateareinagreementwiththe majority of recentstudiesshowing thatlowosmolarionicCMinhibitplatelet activation and coagulation (1,4,5). The main controversyin this fieldofinvestigation concernsthe effectof non-ionicCMon plateletfunction.AlbaneseandOgawaand colleaguesshowed that non-ionicCMenhanced plateletdegranulation and aggregation (23,24),whilst Laffanetal. demonstrated thatthe non-ionicCM did notaugmentplateletactivation (10).…”

Section: Discussionmentioning

confidence: 99%

Contrast medium attenuates platelet activation and platelet-leukocyte cross–talk

Ji¹,

Ghaly²,

Hjemdahl³

et al. 2005

Thromb Haemost

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The influence of ionic and non-ionic contrast media (CM) on platelet and leukocyte activation and platelet-leukocyte crosstalk was investigated in hirudinized whole blood. The blood was incubated with and without the ionic CM ioxaglate and the nonionic CM iodixanol at 37 degrees C for 5 min, without or with stirring. Platelet and leukocyte activation and platelet-leukocyte aggregation were measured using whole blood flow cytometry. When blood samples were pre-incubated in the presence of 2%, 5%, and 10% of CM without stirring, both ioxaglate and iodixanol had little effect on unstimulated samples, but dose-independently decreased 1 microM ADP-induced platelet P-selectin expression and fibrinogen binding, and thus platelet-leukocyte aggregate formation. Ioxaglate had little effect on leukocyte CD11b expression, whilst iodixanol slightly enhanced resting and N-formyl-methionyl-leucyl-phenylalanine (fMLP; 0.1 microM)-stimulated leukocyte CD11b expression. Blood samples were also incubated with stirring to investigate the impact of CM (5% of ioxaglate or iodixanol) on platelet-leukocyte cross-talk. Collagen induced marked platelet activation and platelet-leukocyte aggregation, and subsequently elevated leukocyte CD11b expression. The latter was attenuated by ioxaglate and iodixanol, and was accompanied by reduced platelet-leukocyte aggregation. In conclusion, the CM ioxaglate and iodixanol attenuate platelet activation and platelet-leukocyte cross-talk. Inhibitory effects of the contrast agents on this cross-talk are apparently exerted by reducing heterotypic conjugation, and may be beneficial in connection with PCI.

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“…However, evidence is more limited using selective coronary circulation samples. For example, Albanese et al [8] showed no clear effect of iohexol contrast on coronary sinus blood following angiography, though the group postulated that sampling coronary artery blood may provide a more accurate measure of platelet activity. In practice, an enhanced thrombogenicity of a widely used non-ionic low osmolar contrast agent (Iomeprol 300, Bracco, Milan, Italy), may have clinical implications for the patient during invasive procedures.…”

Section: Introductionmentioning

confidence: 99%

Effects of low osmolar contrast (iomeprol) on haemorheology and platelet activation in patients with coronary artery disease

Jaumdally

Varma

MacFadyen

et al. 2007

J Thromb Thrombolysis

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Effects of ionic and nonionic contrast media on in vitro and in vivo platelet activation

Cited by 25 publications

References 22 publications

Differential effects of three radiographic contrast media on platelet aggregation and degranulation: implications for clinical practice?

Differential effects of three radiographic contrast media on platelet aggregation and degranulation: implications for clinical practice?

Contrast medium attenuates platelet activation and platelet-leukocyte cross–talk

Effects of low osmolar contrast (iomeprol) on haemorheology and platelet activation in patients with coronary artery disease

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