Effects of ionic liquids on membrane fusion and lipid aggregation of egg-PC liposomes

Galletti, Paola; Malferrari, Danilo; Samorì, Chiara; Sartor, Giorgio; Tagliavini, Emilio

doi:10.1016/j.colsurfb.2014.11.021

Cited by 31 publications

(17 citation statements)

References 54 publications

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“…In addition, the effect of various imidazolium, pyridinium, and pyrrolidinium ILs toward eggPC liposomes was recently investigated and the results suggest that all tested ILs induced fusion and 1-dodecyl-3-methylimidazolium bromide, which had the longest alkyl chain length, disrupted the liposomes when the concentration was above 1 mM. However, pyridinium cation was proven to be the most membrane active [47]. Furthermore, it was shown that imidazolium ILs with oxygenated lateral chains induced smaller biological effects at different biological levels [48].…”

Section: Effect Of Ionic Liquids On Analyte-liposome Interactionsmentioning

confidence: 97%

Effect of ionic liquids on the interaction between liposomes and common wastewater pollutants investigated by capillary electrophoresis

Ruokonen

Duša

Lokajová

et al. 2015

Journal of Chromatography A

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Section: Effect Of Ionic Liquids On Analyte-liposome Interactionsmentioning

confidence: 97%

Effect of ionic liquids on the interaction between liposomes and common wastewater pollutants investigated by capillary electrophoresis

Ruokonen

Duša

Lokajová

et al. 2015

Journal of Chromatography A

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“…Thus, any alteration or disruption of the biomembrane can have serious or even lethal consequences for the organism. It has recently been reported that imidazolium based ILs can induce membrane fusion [14] and alter membrane permeability [15]. Much in the same way as certain amphipathic α-helical peptides, positively charged surfactants, and other peptides [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 96%

Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance

Duša

Ruokonen

Petrovaj

et al. 2015

Colloids and Surfaces B: Biointerfaces

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The worldwide use of ionic liquids (ILs) is steadily increasing, and even though they are often referred to as "green solvents" they have been reported to be toxic, especially toward aquatic organisms. In this work, we thoroughly study two phosphonium ILs; octyltributylphosphonium chloride ([P8444]Cl) and tributyl(tetradecyl)phosphonium chloride ([P14444]Cl). Firstly, the critical micelle concentrations (CMCs) of the ILs were determined with fluorescence spectroscopy and the optical pendant drop method in order to gain an understanding of the aggregation behavior of the ILs. Secondly, a biomimicking system of negatively charged unilamellar liposomes was used in order to study the effect of the ILs on biomembranes. Changes in the mechanical properties of adsorbed liposomes were determined by quartz crystal microbalance (QCM) measurements with silica coated quartz crystal sensors featuring a polycation layer. The results confirmed that both ILs were able to incorporate and alter the biomembrane structure. The membrane disrupting effect was emphasized with an increasing concentration and alkyl chain length of the ILs. In the extreme case, the phospholipid membrane integrity was completely compromised.

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“…Lipids, disengaged from the disrupted liposomes, have further been shown to aggregate with surface‐active imidazolium based ILs forming IL‐lipid micelles, as seen also with other surfactants . In addition, imidazolium‐ and cholinium‐based ILs with lactate anion and imidazolium, pyrrolidinium, and pyridinium based ILs have been shown to even induce liposome fusion.…”

Section: Introductionmentioning

confidence: 97%

Correlation between Ionic Liquid Cytotoxicity and Liposome–Ionic Liquid Interactions

Ruokonen

Sanwald

Robciuc

et al. 2018

Chemistry A European J

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This study aims at extending the understanding of the toxicity mechanism of ionic liquids (ILs) using various analytical methods and cytotoxicity assays. The cytotoxicity of eight ILs and one zwitterionic compound was determined using mammalian and bacterial cells. The time dependency of the IL toxicity was assessed using human corneal epithelial cells. Hemolysis was performed using human red blood cells and the results were compared with destabilization data of synthetic liposomes upon addition of ILs. The effect of the ILs on the size and zeta potential of liposomes revealed information on changes in the lipid bilayer. Differential scanning calorimetry was used to study the penetration of the ILs into the lipid bilayer. Pulsed field gradient nuclear magnetic resonance spectroscopy was used to determine whether the ILs occurred as unimers, micelles, or if they were bound to liposomes. The results show that the investigated ILs can be divided into three groups based on the cytotoxicity mechanism: cell wall disrupting ILs, ILs exerting toxicity through both cell wall penetration and metabolic alteration, and ILs affecting solely on cell metabolism.

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Effects of ionic liquids on membrane fusion and lipid aggregation of egg-PC liposomes

Cited by 31 publications

References 54 publications

Effect of ionic liquids on the interaction between liposomes and common wastewater pollutants investigated by capillary electrophoresis

Effect of ionic liquids on the interaction between liposomes and common wastewater pollutants investigated by capillary electrophoresis

Ionic liquids affect the adsorption of liposomes onto cationic polyelectrolyte coated silica evidenced by quartz crystal microbalance

Correlation between Ionic Liquid Cytotoxicity and Liposome–Ionic Liquid Interactions

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