Effects of Ionizing Radiation on the Bone Induction Principle in the Matrix of Bone Implants

Buring, Klas; Urist, Marshall R.

doi:10.1097/00003086-196711000-00019

Cited by 49 publications

(24 citation statements)

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“…In some studies, irradiation has been found to inhibit (Buring and Urist 1967) or only partially destroy (Munting et al 1988, Schwarz et al 1988, Ijiri et al 1994, Zhang et al 1997) the osteoinductive capacity. In others, no effect of irradiation on the osteoinductive capacity has been found (Wientroub and Reddi 1988, Andriano et al 2000, Ripamonti et al 2000, Pekkarinen et al 2005.…”

Section: Histomorphometrymentioning

confidence: 99%

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

2007

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Background Gamma irradiation has been widely used for sterilization of bone allografts. However, gamma irradiation alters proteins. This is favorable when it reduces immunogenicity, but is undesirable when osteoinductive proteins are damaged. Although the effect of gamma irradiation on BMPs has been studied, the effect of irradiation on the process of incorporation of morselized bone chips remains unclear. We studied the effects of sterilization by gamma irradiation on the incorporation of impacted morselized allografts.Methods Bone chambers with impacted allografts, rinsed impacted allografts, allografts that were rinsed and subsequently irradiated, and an empty control were implanted in proximal medial tibiae of goats. Incorporation was evaluated using histology and histomorphometry.Results Histology revealed evidence of bone graft incorporation, which proceeded in a similar way in unprocessed, rinsed, and both rinsed and irradiated bone grafts. After 12 weeks, no difference in bone and tissue ingrowth was found between the unprocessed, the rinsed, and the rinsed and subsequently irradiated allografts. The amount of unresorbed graft remnant was highest in the unprocessed bone grafts.Interpretation We conclude that sterilization with gamma irradiation does not influence the incorporation of impacted rinsed bone allografts.

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Section: Histomorphometrymentioning

confidence: 99%

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

2007

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“…Spira et al in 1970 were the first to use irradiation to destroy the tumor cells. Irradiation is commonly used to sterilize bone for storage in bone banks, to study the effects of different doses of irradiation on chemical components of bone matrix (Turner et al 1956), and on the biologic property known as the bone induction principle (Buring and Urist 1967) as well as for postoperative radiotherapy in combination with bone grafts (Nathanson andBackstrom 1978, Roy-Camille et al 198 1, De Santis et al 1990). Doses exceeding 10 kGy significantly inhibited the osteoinductive activity of demineralized bone matrix, and irradiation of mineralized bone affects its potential osteoinductive property to an even greater degree than irradiation of the demineralized matrix (Urist and Hernandez 1974).…”

mentioning

confidence: 99%

Extracorporeal irradiation and incorporation of bone grafts: Autogeneic cortical grafts studied in rats

Voggenreiter¹,

Ascherl

Blümel

et al. 1996

Acta Orthopaedica Scandinavica

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“…Processing procedures also are known to play an important role in the osteoinductive properties of a DBM. Preprocess handling, 36 varying demineralization times, 37 final particle size, 38,39 and terminal sterilization 40,41 are among the many factors that may affect osteoinductivity.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of the osteoinductivity of two formulations of human demineralized bone matrix

Takikawa

Bauer

Kambic

et al. 2003

J Biomedical Materials Res

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In the United States, demineralized bone matrix (DBM) is considered a transplantable tissue and therefore is regulated primarily by the American Association of Tissue Banks. Even though DBM is not subjected to the same regulations relative to performance claims as medical devices are, one would expect different processing methods might yield DBM preparations of different osteoinductive potential. The purpose of this study was to use an established athymic rat model to compare the osteoinductive properties of two commercially available human DBMs prepared using different methods but having essentially identical product claims. Sixteen female athymic rats were used to test equivalent volumes of two lots each of Grafton Putty (Osteotech, Inc., Eatontown, NJ), Osteofil (Regeneration Technologies, Inc., Alachua, FL), and rat DBM. At 28 days after implantation, qualitative and semiquantitative microscopy showed no significant differences in bone formation between the two lots from each source, but rat DBM produced significantly more bone than Grafton, which produced significantly more bone than Osteofil. Our results suggest that methods of graft processing may represent a greater source of variability than do differences among individual donors. Whether these differences relate to methods of demineralization, carrier, dose of DBM per volume, or to some other factor remains to be determined.

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Effects of Ionizing Radiation on the Bone Induction Principle in the Matrix of Bone Implants

Cited by 49 publications

References 0 publications

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

Irradiation has no effect on the incorporation of impacted morselized bone: A bone chamber study in goats

Extracorporeal irradiation and incorporation of bone grafts: Autogeneic cortical grafts studied in rats

Comparative evaluation of the osteoinductivity of two formulations of human demineralized bone matrix

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