The present investigation was conducted at the nursery of ornamental plants, faculty of agriculture, Minia university, during the two growing seasons of October, 2020/2021 and 2021/2022, to determine the effect of soil application of humic acid at (0, 100, 200 and 400 ppm) and foliar application of vitamin E at (0, 25, 50 and100 ppm) as well as the control treatment on growth and yield of chia (Salvia hispanica, L.) plants.The obtained data revealed that all concentrations of humic acid significantly increased all studied parameters: vegetative growth ]plant height, stem diameter, total number of branches, and leaves/plant, leaf area and herb fresh and dry weight), yield components (number of inflorescences /plant, seeds yield /plant, feddan and 1000 seed weight) fixed oil productivity ]fixed oil percentage and yield [ and chemical constituents ] NPK concentrations and photosynthetic pigments) as compared with control treatment. In this study, humic acid at 400 ppm was the most effective in this concern. Also, the same results were obtained with the treatments of vitamin E at different concentrations for the previous studied characters. The best treatment was 50 ppm.The best interaction treatment for the highest yield of the chia plant was obtained with the treatment of humic acid at a 400 ppm combined with vitamin E at a concentration of 50 ppm.