Effects of Ischemia on Voiding Function and Nerve Growth Factor of the Rat Urinary Bladder

Abstract

The present results will indicate that the voiding function deteriorated by acute ischemia is temporarily compensated by detrusor hyperrefrexia, which may be attributable to an enhanced NGF synthesis, and then improves by the development of collateral blood circulation.

Expression of nerve growth factor immunoreactivity and messenger RNA in ischemic urinary bladder

“…However, Ishida et al 9 using bilateral internal iliac artery ligation and enzyme immunoassay found total bladder NGF protein increased 2.4 times of the control at 1 day and then decreased to the normal level from 1 week. Gill et al 2 observed that partial recovery of detrusor muscle from ischemic injury occurred at 2 and 4 weeks after unilateral vesical artery ligation.…”

“…Although Gill et al 2 did not mention if there was any detrusor recovery after bilateral vesical artery ligation, our data suggested that recovery of detrusor muscle after bilateral vesical artery ligation may need more than 4 weeks, because the expression of NGF immunoreactivity did not recover to that of sham-operated level at 4 weeks. Ishida et al 9 demonstrated that blood flow decreased to 18% of the control immediately after bilateral internal iliac artery ligation, but gradually recovered to 66% of the control after 4 weeks. They suggested that the improved blood supply of bladder may be attributed to the development of collateral Neurourology and Urodynamics DOI 10.1002/nau Fig.…”

“…8 To date few data have mentioned about the correlation between ischemic bladder damage and NGF expression. A previous study reported that the voiding function deteriorated after acute ischemia can be temporarily compensated by detrusor overactivity, which may be attributed to an enhanced NGF synthesis; 9 however, the NGF mRNA expression following bladder ischemia has not been studied. In this study, we adopted an ischemic bladder model induced by ligation of bilateral vesical arteries to investigate the acute and mid-term effects of bladder ischemia on the temporal expression of NGF immunoreactivity and mRNA.…”

Ätiologie und Pathogenese der Blasenüberaktivität

“…Im Tierexperiment wurde versucht, eine Organischämie mittels uni-bzw. bilateraler Ligatur der A. iliaca interna bei Ratten zu induzieren, was zu gesteigerter Miktionsfrequenz, dem Auftreten von Detrusorhyperaktivitäten und Reduktion des maximalen Detrusordruckes führte [26]. [27,28].…”

“…Ursächlich werden Urothelschä-den, ein submuköses und intramuskulä-res Ödem sowie eine glattmuskuläre Degeneration beschrieben [26,27,28]. Die Blasenüberaktivität wird einer Neurodegeneration mit konsekutivem Anstieg des NGF zugeschrieben [26,29]. Untersuchungen am Menschen hinsichtlich des Zusammenhangs von Blasenperfusion und Auftreten einer DHA liegen bislang nicht vor.…”