Effects of isoflavone derivatives on the production of inflammatory cytokines by synovial cells

Abstract

The present study investigated the effects of isoflavone derivatives (daidzein, genistein and glycitein) on the production of inflammatory cytokines (IL-6 and IL-8) by IL-1β-stimulated synovial cells. Synovial MH7A cells were stimulated with IL-1β in the absence or presence of isoflavone derivatives, and IL-6 and IL-8 production was measured by ELISA. The results of the present study indicated that daidzein significantly inhibited the production of IL-6, but not IL-8. Conversely, neither genistein nor glycitein exerted any inhibitory effects on the production of IL-6 or IL-8 by IL-1β-stimulated synovial cells. To elucidate the molecular mechanisms underlying the daidzein-mediated inhibition of IL-6 production, the present study examined the effects of daidzein on the phosphorylation (activation) of NF-κB p65, ERK1/2 and p38 MAPK. Daidzein significantly inhibited the phosphorylation of NF-κB p65 and ERK1/2, but not p38 MAPK in IL-1β-stimulated MH7A cells. The present study revealed that among the isoflavone derivatives examined (daidzein, genistein and glycitein), daidzein inhibited the production of IL-6, but not IL-8, by IL-1β-stimulated synovial MH7A cells via the suppression of NF-κB p65 and ERK1/2 activation. Collectively, these results suggested that daidzein may have potential as a therapeutic agent for the treatment of arthritic disorders through its anti-inflammatory effects via the inhibition of IL-6 production.

Cellular and Transcriptional Response of Human Astrocytes to Hybrid Protein Materials

“…59 Further study showed that the isoflavone derivative daidzein inhibited IL-6 expression in synovial cells. 60 We now report the apparent regulation of CLL2 and CXC chemokines by soy protein in astrocytes. The reduced production of chemokines by astrocytes may improve the regeneration of wounded neural tissue by grafting of the SPI− collagen hybrid scaffold.…”

“…The secretion of chemokines IL-1β, IL-6, and IL-8 by MH7A cells was reduced by the treatment of the soy isoflavone derivative genistein . Further study showed that the isoflavone derivative daidzein inhibited IL-6 expression in synovial cells . We now report the apparent regulation of CLL2 and CXC chemokines by soy protein in astrocytes.…”

Simultaneous quantitative analysis and in vitro anti-arthritic effects of five polyphenols from Terminalia chebula

“…Fibroblast-like synoviocytes synthesizes and secretes mediators of inflammation including IL-6 and IL-8 ( Georganas et al, 2000 ). Furthermore, a large body of literature has proposed that IL-6 and IL-8, two pro-inflammatory factors were generally chosen as pro-inflammatory cytokines ( Li et al, 2014 ; Mijiti et al, 2021 ). Therefore, in this study, IL-1β-induced MH7A cells were selected to construct an in vitro inflammation model to examine the effects of the polyphenols on the pro-inflammatory factors IL-6 and IL-8.…”