Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows

Abstract

In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 μg/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 μg/L in the CTRL group to 186.30 μg/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings.

Dosage‐dependent effects of monensin on the rumen microbiota of lactating dairy cattle

“…The majority of these sequences were associated with the phyla Bacteroidetes (58.9%), Firmicutes (31.4%), and Proteobacteria (3.6%). These results are similar to previous studies that examined 16S rRNA genes libraries derived from lactating dairy cows (Kasparovska et al, ; Pitta et al, ). The library derived from the cows fed the low dose of monensin had no significant change ( p > 0.05) in the percentage of sequences associated with any of the phyla.…”

In vitro metabolism of red clover isoflavones in rumen fluid

“…Knowledge in this area is scarce. According to Kasparovska et al (2016) In our study, the concentrations of equol combined across the diet types and incubation times were not different between the levels of added clover extract, which is in concordance with our previous study with soybean extract (Trnková et al, 2018). If, however, we examine the diet types separately, changes in equol production in dependence on added isoflavone amount become apparent.…”

Variation of Phytoestrogen Content and Major Agronomic Traits in Alfalfa (Medicago sativa L.) Populations

“…Feeding animals on forages with high isoflavone concentrations have desirable impact on quicker weight gain of lambs, as well as on cows' milk production [18,19]. A strong association between formononetin intake and equol concentration in plasma was demonstrated, so that cows' milk can be considered as a source of equol in human nutrition [20][21][22][23][24][25]. On the other hand, consumption of forages with high concentrations of formononetin and coumestrol has shown to cause temporary or permanent reproductive problems in some ruminants, mostly in sheep and cattle [26][27][28][29][30].…”