Effects of Isotonic Crystalloid Resuscitation on Fluid Compartments in Hemorrhaged Rats

Moon, Paula F.; Hollyfield-Gilbert, Michele A.; Myers, Tamara; Kramer, George C.

doi:10.1097/00024382-199411000-00010

Cited by 32 publications

(19 citation statements)

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“…The doses used here are based on previously published studies showing activation of V-ATPase-proton secretion in renal intercalated cells by these agonists in vivo (43,63,64). The final aldosterone concentration injected into the animals was estimated to be ϳ1.55 nM, based on an average extracellular fluid volume of 103 ml for an adult rat (21,33,36,60). This dose corresponds to the values reached under conditions of dietary sodium restriction or hyperkalemia in humans (45).…”

Section: Methodsmentioning

confidence: 99%

Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis

Roy

Hill

Ruan

et al. 2013

American Journal of Physiology-Cell Physiology

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ϩ -ATPase (V-ATPase) and acidify the lumen of the epididymis, a process that is essential for male fertility. The renin-angiotensin-aldosterone system (RAAS) regulates fluid and electrolyte balance in the epididymis, and a previous study showed binding of aldosterone exclusively to epididymal clear cells (Hinton BT, Keefer DA. Steroid Biochem 23: 231-233, 1985). We examined here the role of aldosterone in the regulation of V-ATPase in the epididymis. RT-PCR showed expression of the mineralocorticoid receptor [MR; nuclear receptor subfamily 3, group C member 2 (NR3C2)] and 11-␤-dehydrogenase isozyme 2 (HSD11␤2) mRNAs specifically in clear cells, isolated by fluorescence-activated cell sorting from B1-enhanced green fluorescent protein (EGFP) mice. Tail vein injection of adult rats with aldosterone, 1,2-dioctanoyl-snglycerol (DOG), or 8-(4-chlorophenylthio)-cAMP (cpt-cAMP) induced V-ATPase apical membrane accumulation and extension of V-ATPaselabeled microvilli in clear cells in the caput epididymis but not in the cauda. V-ATPase activity was measured in EGFP-expressing clear cells using the intracellular pH (pH i)-sensing dye seminaphthorhodafluor-5F-5-(and 6)-carboxylic acid, acetoxymethyl ester acetate (SNARF-5F). Aldosterone induced a rapid increase in the rate of Na ϩ -and bicarbonate-independent pH i recovery following an NH4Cl-induced acid load in clear cells isolated from the caput but not the cauda. This effect was abolished by concanamycin A, spironolactone, and chelerythrine but not myristoylated-protein kinase inhibitor (mPKI) or mifepristone. Thus aldosterone increases V-ATPasedependent proton secretion in clear cells in the caput epididymis via MR/NR3C2 and PKC activation. This study, therefore, identifies aldosterone as an active member of the RAAS for the regulation of luminal acidification in the proximal epididymis. aldosterone; nongenomic; epididymis; clear cell; V-ATPase pump THE EPIDIDYMIS IS AN IMPORTANT male reproductive organ involved in the maturation and storage of spermatozoa. The pseudostratified epithelium of the epididymis is composed of numerous cell types, including principal, basal, and clear cells that function in concert to tightly regulate the luminal environment in which sperm transit (51). One major function of clear cells in the epididymis is to acidify the luminal fluid via the vacuolar proton-pumping H ϩ -ATPase (V-ATPase), which is located on the apical membrane (6,7,13,14). Under resting conditions, a large fraction of V-ATPase resides in a subapical pool of vesicles, and upon activation the V-ATPase is trafficked to the apical membrane. This is accompanied by an increase in apical membrane surface area resulting in the formation and elongation of apical microvillar projections. Thus microvilli elongation directly correlates with V-ATPase activity and has been used as a measure of clear cell activation (6,7,19,39,49). The importance of clear cells to male fertility has been demonstrated, as impairment of V-ATPase activity by genetic manipulation or by environmental factor...

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Section: Methodsmentioning

confidence: 99%

Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis

Roy

Hill

Ruan

et al. 2013

American Journal of Physiology-Cell Physiology

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“…3,4,29,30 The role of early resuscitation with sodium chloride solution, hypertonic saline, and Ringer's lactate solution on T-cell functions and tissue injury has been evaluated previously; promising results were reported with early resuscitations with hypertonic saline and Ringer's solution. 5,6,30,31 Hepatic and pulmonary apoptosis after HS was reduced with the modification of Ringer's solution. 30,31 Recently, rapid restoration of microcirculatory blood flow with hyperviscous and hyperoncotic solutions has been reported to lower the transfusion trigger in resuscitation from HS.…”

Section: Discussionmentioning

confidence: 99%

“…2,3 Although trauma and hemorrhage are well known to produce tissue hypoxia, systemic inflammatory response, and organ dysfunction, the mechanisms responsible for organ-specific injury and/or MOF after nonresuscitated HS are not clear. [4][5][6] However, three major events contribute to the development of MOF in HS: (1) progressive and persistent splanchnic vasoconstriction and hypoperfusion; (2) a systemic inflammatory response derived from the gut; and (3) an obligatory fluid sequestration. [7][8][9][10][11][12] Known contributors to the development of microcirculatory failure-induced MOF are increased mesenteric bioactivity (which has been reported to be mediated by nuclear factor-kappa B [NF-κB]), hypoxic pulmonary vasoconstriction, and local production of pro-inflammatory cytokines.…”

Section: Introductionmentioning

confidence: 99%

The role of erythropoietin in hemorrhagic shock-induced liver and renal injury in rats

Algın¹,

Hacioğlu²,

Yaylak³

et al. 2008

Adv Therapy

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“…Nas décadas subsequentes, com o franco avanço tecnológico, estudos mais complexos foram realizados e novos efeitos sistêmicos originados da reposição volêmica com grandes volumes de cristalóides foram constatados, incluindo aumento do volume intersticial nos tecidos viscerais e cardíacos [Moon et al 1994], síndrome compartimental abdominal [Maxwell et al 1999e Madigan et al 2008, síndrome compartimental em extremidades sem injúrias [Ablove et al 2006] e derrame pericárdico [Hashim et al 2002]. Uma revisão mais recente ressalta, além dos efeitos clínicos deletérios, os danos celulares, metabólicos e imunológicos originados da ressuscitação volêmica agressiva [Cotton et al 2006].…”

Section: Os Ensinamentos Da Guerraunclassified

Repercussões celulares e imunológicas dos isômeros de ringer lactato na ressuscitação volêmica humana

Monzo

Maleck

2016

R. Saúde

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ResumoA hemorragia é responsável por 40% das mortes por trauma e é intenso o debate sobre o manejo correto destes pacientes. O Ringer Lactato é a solução considerada ideal para ressuscitação volêmica humana. Contudo, mesmo uma substância consagrada como o Ringer Lactato promove uma série de efeitos celulares e imunológicos deletérios ao organismo. O presente estudo propôs uma revisão literária acerca da atividade dos isômeros da solução de Ringer Lactato no organismo humano com o objetivo de entender melhor a ação destes isômeros nos mecanismos imunológicos a fim de minimizar os efeitos colaterais decorrentes de sua utilização. Tal revisão se deu através de pesquisa sistemática em bases de dados na área da saúde (Medline, Lilacs, Bireme, Pubmed e Scielo.), contemplando as publicações científicas dos últimos 50 anos. Palavras Cellular and immunological effects of ringer's lactate isomers in human volume resuscitation Repercussões celulares e imunológicas dos isômeros de ringer lactato na ressuscitação volêmica humana AbstractThe bleeding is responsible for 40% of the deaths from trauma and the debate on the correct management of these patients is intense. The Lactate Ringer solution is considered ideal for volume resuscitation. However, even a substance consecrated as the Ringer Lactate promotes a series of cellular and immunological effects harmful to the body. This study proposed a literary review on the activity of isomers of Lactate Ringer solution in the human body in order to better understand the action of these isomers in immunological mechanisms to minimize the side effects resulting from its use. Such review will be through systematic research on health databases (Medline, Lilacs, Scielo, PubMed and Bireme.), including scientific publications of the last 50 years.

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Effects of Isotonic Crystalloid Resuscitation on Fluid Compartments in Hemorrhaged Rats

Cited by 32 publications

References 0 publications

Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis

Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis

The role of erythropoietin in hemorrhagic shock-induced liver and renal injury in rats

Repercussões celulares e imunológicas dos isômeros de ringer lactato na ressuscitação volêmica humana

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