Effects of LAB inoculant and cellulase on the fermentation quality and chemical composition of forage soybean silage prepared with corn stover

Abstract

The objective of the present study was to evaluate the silage quality of forage soybean‐corn stover silage in the south of China. Forage soybean were combined manually with corn stover by ratios of R1 (25:75), R2 (50:50) and R3 (75:25) in fresh weight (FM) and ensiled in bag silos for 60 days at room temperature (20–25°C). All silages were treated without (control) or with lactic acid bacteria (LAB) inoculant (G, Lactobacillus plantarum and Lactobacillus buchneri, 105 cfu/g, of FM), cellulase (C, 0.02% of FM) and C + G. R1 silage had appropriate V‐score value with high lactic acid content and low pH value and ammonia‐N (NH3‐N) content, undetectable levels of propionic acid (PA) and butyric acid (BA). C and C + G reduced undesirable microorganisms of aerobic bacteria, yeasts and molds, resulting in improved nutritive value of R2 silage, as indicated by lower neutral detergent fiber and acid detergent fiber contents, and higher crude protein content, in vitro ruminal dry matter digestibility, gross energy and metabolizable energy values as compared to the control. Additives did not improve the quality of R3 silage with high pH value and NH3‐N content, BA and PA contents and undesirable microorganism counts. These results confirmed that high‐quality silage produced from mixtures (25:75) of forage soybean and corn stover in the south of China; additives (especially for C + G) could enhance the silage quality of forage soybean‐corn stover (50:50); and high proportion (>75%) of forage soybean was difficult to ensile.

Effects of Bacillus subtilis and its metabolites on corn silage quality

“…(2019), Cheng et al . (2020) and Martínez et al . (2020) reported improved fibre digestibility of corn silages treated with fibrolytic enzymes.…”

“…(2017) and Cheng et al . (2020) showed that the addition of enzymes only increased CP content. Microbial inoculants can modify the concentration of DM and CP in silages by improving the fermentation pattern, increasing the production of organic acids and other substances that inhibit the growth of undesirable micro‐organisms.…”

“…2017; Cheng et al . 2020; Martínez et al . 2020) and also possible, particle sizes and arrangement of the vegetable tissue.…”

Effects of Delayed Harvest and Additives on Fermentation Quality and Bacterial Community of Corn Stalk Silage

“…This might be due to the lower pH in LP-treated silage and the antibacterial ability of BF. NH 3 -N is an important indicator for CP degradation, and its content in well-preserved silage should be lower than 10% total N ( Chen et al, 2020b ; Cheng et al, 2021 ). However, the NH 3 -N content of CK on each harvest day was higher than that level.…”

Inhibition of Listeria monocytogenes by bacteriocin-producing Bacillus velezensis isolated from silage

“…Lactic acid bacteria (LAB) have been extensively used in order to inhibit the growth of undesirable microorganisms (Dolenčić Špehar et al, 2020). In addition to microbial control, LAB can also be used to improve the fermentation process in silage, contributing for silage quality (Cheng et al, 2021). Many of LAB are able to produce antimicrobial compounds, such as bacteriocins, that can control the bacterial community during the ensilage process (Contreras-Govea et al, 2011).…”