Effects of Lactate Dehydrogenase on Fissure Caries in Rats

Higham, Susan; Edgar, W.M.

doi:10.1159/000261236

Cited by 6 publications

(2 citation statements)

References 15 publications

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“…The lactate dehydrogenase (LDH) gene was also enriched in caries-free samples, which again concurs with results from the same proteomic study referred to above. Interestingly, a historical study found LDH to delay onset and reduce severity of caries in rats [45]. L-lysine biosynthesis was also over-abundant in caries-free subjects.…”

Section: Discussionmentioning

confidence: 99%

Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children

Al-Hebshi

Baraniya

Chen³

et al. 2018

Journal of Oral Microbiology

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Studies of the microbiome associated with dental caries have largely relied on 16S rRNA sequence analysis, which is associated with PCR biases, low taxonomic resolution, and inability to accurately study functions. Here, we employed whole metagenome shotgun sequencing, coupled with high-resolution analysis algorithm, to analyze supragingival microbiomes from 30 children with or without dental caries. A total of 726 bacterial strains belonging to 406 species, in addition to 34 bacteriophages were identified. A core bacteriome was identified at the species and strain levels. Species of Prevotella, Veillonella, as yet unnamed Actinomyces, and Atopobium showed strongest association with caries; Streptococcus sp. AS14 and Leptotrichia sp. Oral taxon 225, among others, were overabundant in caries-free. For several species, the association was strain-specific. Furthermore, for some species, e.g. Streptococcus mitis and Streptococcus sanguinis, sister strains showed differential associations. Noteworthy, associations were also identified for phages: Streptococcus phage M102 with caries and Haemophilus phage HP1 with caries-free. Functionally, potentially relevant features were identified including urate, vitamin K2, and polyamine biosynthesis in association with caries; and three deiminases and lactate dehydrogenase with health. The results demonstrate new associations between the microbiome and dental caries at the strain and functional levels that need further investigation.

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Section: Discussionmentioning

confidence: 99%

Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children

Al-Hebshi

Baraniya

Chen³

et al. 2018

Journal of Oral Microbiology

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“…This has been supported by the findings of par allel animal studies where LDH used as a dietary ad ditive has been found to delay the onset of carious le sion formation in rats [Higham and Edgar, 1990] and has been implicated by Bibby et al [1985] to reduce the severity of dental caries in rats. The present evi dence suggests that this enzyme warrants further in vestigations as a potential cariostatic agent.…”

Section: Discussionmentioning

confidence: 65%

Extracellular Administration of Lactate Dehydrogenase and Its Effects on Human Plaque pH and Acid Anion Concentrations

Higham¹,

Edgar²

1991

Caries Res

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The effect of lactate dehydrogenase (LDH) on human plaque pH and acid anion concentrations in vivo was investigated. Rinsing with sucrose solutions supplemented with LDH (1 or 2 U/ml) gave rise to reduced pH fall, decreased cH area and an increase in the mean minimum pH when compared with rinsing with sucrose only (p < 0.05). Mean acid anion estimations showed that plaque fluid concentrations of lactate, acetate and proprionate significantly decreased (p < 0.05) following rinsing with sucrose containing LDH at both levels and decreases in succinate when 1 U/ml LDH was present, whereas formate concentrations were only significantly lower when 2 U/ml LDH was added to sucrose rinses. It is thought that the ability of LDH to produce an alteration in the acid end-products of plaque metabolism may provide some protection against dental caries.

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Effects of lactate dehydrogenase and nicotinamide adenine dinucleotide on human dental plaque pH and acid anion concentrations

1995

Archives of Oral Biology

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Effects of Lactate Dehydrogenase on Fissure Caries in Rats

Cited by 6 publications

References 15 publications

Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children

Metagenome sequencing-based strain-level and functional characterization of supragingival microbiome associated with dental caries in children

Extracellular Administration of Lactate Dehydrogenase and Its Effects on Human Plaque pH and Acid Anion Concentrations

Effects of lactate dehydrogenase and nicotinamide adenine dinucleotide on human dental plaque pH and acid anion concentrations

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