Effects of lactic acid bacteria cultures on pathogenic microbiota from fish

Abstract

Headline: Inhibition of pathogenic microbiota SUMMARYThe concrete nature of the probiotic effects that the presence of microorganisms (especially lactic acid bacteria: LAB) exercise on larval cultures of fish, it is not well defined, being been able to attribute to different factors or action mechanisms. In fact, the production of diverse antibacterial metabolites (bacteriocins in particular) by many LAB forms are able to constitute the basis of these probiotic effects, as repeatedly described in the literature. Accordingly, the inhibition of fish pathogenic species by extracts of LAB constitutes a rapid method for detecting potential probiotics. By studying the response of four common pathogens of turbot (Scophthalmus maximus) to nine potential probiotics, the diversity and mechanisms of effectors in the probiotics were demonstrated to present complex profiles dose-response and non-treatable with conventional models. Proposed modifications allow satisfactory fits and the calculation of useful parameters in the comparison of activities. The results showed that lactic and acetic acid, and not the bacteriocins, are responsible for effects (inhibitory or stimulatory depending on the concentrations considered) in all the cases studied.

In vitro selection of bacteria with potential for use as probiotics in marine shrimp culture

“…inhibition halos against the Gram-positive bacteria analyzed in the present study. However, bacteriocins do not offer significant action against Gram-negative bacteria (Vázquez et al, 2005). The growth inhibition halos formed against Gram-negative bacteria are associated with other compounds produced by lactic acid bacteria, such as hydrogen peroxide (Sugita et al, 2007), organic acids, and acetic acid (Vázquez et al, 2005).…”

“…This stage normally involves the isolation of dozens or even hundreds of strains. Following isolation, several in vitro selection tests are usually performed, such as pathogen inhibition (Guo et al, 2009), production of digestive enzymes (Ochoa-Solano, 2006), production of antimicrobial substances (Vázquez et al, 2005;Sugita et al, 2007), growth rate (Vine et al, 2004a), and ability to adhere to the intestinal epithelium (Vine et al, 2004b), in order to select certain strains for in vivo assays.…”

In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides

“…Most probiotics used in aquaculture belong to the lactic acid bacteria, to the genus Bacillus, or to the genus yeast, although other genera or species have also been mentioned (Bogut et al, 1998;Carnevali et al, 2006;Planas et al, 2006;Vázquez et al, 2005). Few studies have been carried out on the potential of photosynthetic bacteria (PSB), Rhodopseudomonas palustris and Rhodobacter sphaeroides, as probiotics in aquaculture.…”

Antibiotics in Aquaculture – Use, Abuse and Alternatives

“…The most studied are lactic acid bacteria (LAB) (Lactobacillus, Carnobacterium, Enterococcus, Lactococcus, Micrococcus, Streptococcus, and Weissella) (Balcazar et al, 2008;Balcázar et al, 2007;Hagi & Hoshino, 2009;Pérez-Sánchez et al, 2011;Vazquez et al, 2005;Villamil et al, 2002), Bacillus (Ai et al, 2011;Antony et al, 2011;Balcázar & Rojas-Luna, 2007;Bandyopadhyay & Das Mohapatra, 2009;Ochoa-Solano & Olmos-Soto, 2006;Liu et al, 2009;Nakayama et al, 2009;Newaj-Fyzul et al, 2007;Olmos et al, 2011;Salinas et al, 2005;Sun et al, 2010;Vaseeharan & Ramasamy, 2003), Vibrio (Fjellheim et al, 2007;Thompson et al, 2010), Pseudomonas (Abd El-Rhman et al, 2009;Chythanya, 2002;Das et al, 2006;Preetha et al, 2007;Ström-Bestor, Wiklund, 2011), and Aeromonas (Irianto et al, 2003;Lategan et al, 2006;. Yeasts (Saccharomyces, Debaryomyces) (Abdeltawwab et al, 2008;Reyes-Becerril et al, 2008;Tovar-Ramírez et al, 2010), bacterial spore formers (Hong et al, 2005) and recently Actinobacteria have also generated interest due to their high metabolic potential (Das et al, 2010;You et al, 2007).…”